General Stability | Organism |
---|---|
2-mercaptoethanol does not stabilize | Chlamydomonas reinhardtii |
20% polyethylene glycol stabilizes during purification | Chlamydomonas reinhardtii |
dithiothreitol does not stabilize | Chlamydomonas reinhardtii |
EDTA stabilizes | Chlamydomonas reinhardtii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cl- | competitive inhibitor with respect to dihydroxyacetone phosphate | Chlamydomonas reinhardtii | |
dihydroxyacetone phosphate | substrate inhibition above 1 mM, NADH above 0.2 mM; substrate inhibition only at high concentration, over 1 mM | Chlamydomonas reinhardtii | |
L-glycerol 3-phosphate | competitive inhibitor to dihydroxyacetone phosphate, non-competitive to NADH | Chlamydomonas reinhardtii | |
N-ethylmaleimide | 1 mM results in 50% inhibition, reversible by NADH, inhibition increases in the presence of dihydroxyacetone phosphate and/or glycerol 3-phosphate | Chlamydomonas reinhardtii | |
NAD+ | competitive inhibitor to NADH at physiological concentration; non-competitive to dihydroxyacetone phosphate | Chlamydomonas reinhardtii | |
NADH | at high concentration | Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
dihydroxyacetone phosphate | - |
Chlamydomonas reinhardtii | |
0.016 | - |
NADH | - |
Chlamydomonas reinhardtii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | stroma, soluble | Chlamydomonas reinhardtii | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, calculated from Stokes radius | Chlamydomonas reinhardtii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | Chlamydomonas reinhardtii | oxidation at 25% the rate of dihydroxyacetone phosphate reduction at optimal pH | dihydroxyacetone phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
green alga | - |
Chlamydomonas reinhardtii 11/32-90 | - |
green alga | - |
Purification (Comment) | Organism |
---|---|
dye-affinity chromatography | Chlamydomonas reinhardtii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH + H+ | equilibrium random-bi-bi reaction mechanism | Chlamydomonas reinhardtii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34 | - |
- |
Chlamydomonas reinhardtii |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 2 months | Chlamydomonas reinhardtii |
0°C, t1/2: 50 min | Chlamydomonas reinhardtii |
4°C, 20% v/v, glycerol, purified stable | Chlamydomonas reinhardtii |
4°C, t1/2: 6 h in absence of EDTA and polyethyleneglycol | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | glyceraldehyde-3-phosphate | Chlamydomonas reinhardtii | ? | - |
? | |
additional information | fructose-6-phosphate, fructose-1,6-bisphosphate | Chlamydomonas reinhardtii | ? | - |
? | |
additional information | glucose-6-phosphate, acetaldehyde, oxaloacetate | Chlamydomonas reinhardtii | ? | - |
? | |
additional information | no substrates: dihydroxyacetone | Chlamydomonas reinhardtii | ? | - |
? | |
additional information | glyceraldehyde-3-phosphate | Chlamydomonas reinhardtii 11/32-90 | ? | - |
? | |
additional information | fructose-6-phosphate, fructose-1,6-bisphosphate | Chlamydomonas reinhardtii 11/32-90 | ? | - |
? | |
additional information | glucose-6-phosphate, acetaldehyde, oxaloacetate | Chlamydomonas reinhardtii 11/32-90 | ? | - |
? | |
additional information | no substrates: dihydroxyacetone | Chlamydomonas reinhardtii 11/32-90 | ? | - |
? | |
sn-glycerol 3-phosphate + NAD+ | - |
Chlamydomonas reinhardtii | dihydroxyacetone phosphate + NADH + H+ | - |
r | |
sn-glycerol 3-phosphate + NAD+ | oxidation at 25% the rate of dihydroxyacetone phosphate reduction at optimal pH | Chlamydomonas reinhardtii | dihydroxyacetone phosphate + NADH + H+ | - |
r | |
sn-glycerol 3-phosphate + NAD+ | - |
Chlamydomonas reinhardtii 11/32-90 | dihydroxyacetone phosphate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, calculated from Stokes radius | Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
dihydroxyacetone phosphate reduction | Chlamydomonas reinhardtii |
9.3 | - |
glycerol-phosphate oxidation | Chlamydomonas reinhardtii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
increasing pH from 7.0 to 8.0 causes 80% loss of activity | Chlamydomonas reinhardtii |
7 | - |
stable, increasing from 7.0 to 8.0 causes 80% loss of activity | Chlamydomonas reinhardtii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | activity with dihydroxyacetone phosphate and NADPH 5% of that with NADH | Chlamydomonas reinhardtii | |
NAD+ | - |
Chlamydomonas reinhardtii | |
NADH | - |
Chlamydomonas reinhardtii |