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Literature summary for 1.1.1.79 extracted from

  • Fukuda, H.; Moriguchi, M.; Tochikura, T.
    Purification and enzymatic properties of glyoxylate reductase II from baker's yeast (1980), J. Biochem., 87, 841-846.
    View publication on PubMed
Search for more literature for 1.1.1.79

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide isoenzyme 2, 1 mM, 87% inhibition, isoenzyme 1, 1 mM, 17% inhibition Saccharomyces cerevisiae
iodoacetate isoenzyme 2, 1 mM, 97% inhibition, isoenzyme 1, 1 mM, 18% inhibition Saccharomyces cerevisiae
N-ethylmaleimide 1 mM, 58% inhibition Saccharomyces cerevisiae
p-chloromercuribenzoate isoenzyme 2, 1 mM, complete inhibition, 0.1 mM, 88% inhibition, 29% inhibition in the presence of 1 mM dithiothreitol, 21% inhibition in the presence of 1 mM L-cysteine, isoenzyme 1, 1 mM, 59% inhibition Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0057
-
 NADPH
-
 Saccharomyces cerevisiae
0.43
-
 NADH
-
 Saccharomyces cerevisiae
1.4
-
 Hydroxypyruvate
-
 Saccharomyces cerevisiae
16
-
 glyoxylate
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glyoxylate + NAD(P)H Saccharomyces cerevisiae
-
 glycolate + NAD(P)+
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme 2, protamine sulfate, DEAE-cellulose, hydroxyapatite, Sephadex G-150, phosphocellulose Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
61.7
-
 isoenzyme 2 Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
isoenzyme 2, 4°C, 10 mM, potassium phosphate, pH 7.0, 20% glycerol, 1 week, 10% loss of activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glyoxylate + NAD(P)H
-
 Saccharomyces cerevisiae glycolate + NAD(P)+
-
 ?
glyoxylate + NAD(P)H 7% of activity with glyoxylate Saccharomyces cerevisiae glycolate + NAD(P)+
-
 ?
glyoxylate + NADH + H+
-
 Saccharomyces cerevisiae glycolate + NAD+
-
 r
hydroxypyruvate + NAD(P)H + H+
-
 Saccharomyces cerevisiae glycerate + NAD(P)+
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 isoenzyme 2, 90% loss of activity after 5 min, 20% glycerol protects from inactivation, enzyme retains 65% of its activity after 5 min at 40°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7.5 reduction of hydroxypyruvate Saccharomyces cerevisiae
6 6.3
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NAD(P)H
-
 Saccharomyces cerevisiae