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Literature summary for 1.1.1.79 extracted from

  • Kleczkowski, L.A.
    Kinetics and regulation of the NAD(P)H-dependent glyoxylate-specific reductase from spinach leaves (1995), Z. Naturforsch. C, 50, 21-28.
No PubMed abstract available
Search for more literature for 1.1.1.79

Activating Compound

Activating Compound Comment Organism Structure
phosphate 20 mM, 17% activation Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 5 mM, 37% inhibition, incubation for 1 min Spinacia oleracea
alpha-ketoglutarate 5 mM, 57% inhibition Spinacia oleracea
ATP 5 mM, 28% inhibition Spinacia oleracea
Carbonate 20 mM, 7-16% inhibition Spinacia oleracea
chloride 20 mM, 7-16% inhibition Spinacia oleracea
cyanide 2 mM, 46% inhibition, 20 mM, 92% inhibition Spinacia oleracea
diethyldicarbonate 1 mM, 33% inhibition, incubation for 1 min Spinacia oleracea
dithiothreitol 5 mM, 51% inhibition, incubation for 1 min Spinacia oleracea
glycolate 5 mM, 42% inhibition Spinacia oleracea
iodoacetamide 1 mM, 28% inhibition, incubation for 1 min Spinacia oleracea
NADP+ competitive product inhibition Spinacia oleracea
nitrate 20 mM, 7-16% inhibition Spinacia oleracea
nitrite 20 mM, 7-16% inhibition Spinacia oleracea
PMSF 1 mM, 30% inhibition, incubation for 1 min Spinacia oleracea
pyruvate 5 mM, 33% inhibition Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 NADPH
-
 Spinacia oleracea
0.059
-
 glyoxylate cofactor NADPH Spinacia oleracea
0.3
-
 NADH
-
 Spinacia oleracea
1.1
-
 glyoxylate cofactor NADH Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Spinacia oleracea 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glyoxylate + NAD(P)H Spinacia oleracea
-
 glycolate + NAD(P)+
-
 ?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glyoxylate + NAD(P)H
-
 Spinacia oleracea glycolate + NAD(P)+
-
 ?
glyoxylate + NADH + H+
-
 Spinacia oleracea glycolate + NAD+
-
 r

Cofactor

Cofactor Comment Organism Structure
NAD(P)H highly specific for NADPH Spinacia oleracea
NADPH highly specific for NADPH Spinacia oleracea