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Literature summary for 1.1.1.77 extracted from

  • Boronat, A.; Aguilar,J.
    Rhamnose-induced propanediol oxidoreductase in Escherichia coli: purification, properties and comparison with the fucose-induced enzyme (1979), J. Bacteriol., 140, 320-326.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
L-1,2-propanediol above 25 mM: substrate inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
L-lactaldehyde pH 7.0 Escherichia coli
1.25
-
L-1,2-propanediol pH 9.5 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
2 * 39000, SDS-PAGE Escherichia coli
76000
-
gel permeation chromatography on a calibrated column of Ultrogel AcA 34 under denaturating conditions Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-propane-1,2-diol + NAD+ Escherichia coli
-
(S)-lactaldehyde + NADH
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
crude extract, ammonium sulfate precipitation, heat treatment, ultrogel AcA 34 fractionation, DEAE-Sephadex column chromatography, second ultrogel AcA 34 fractionation Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-propane-1,2-diol + NAD+ acts only on L-isomer Escherichia coli (R)-lactaldehyde + NADH
-
?
(S)-propane-1,2-diol + NAD+
-
Escherichia coli (S)-lactaldehyde + NADH
-
r
(S)-propane-1,2-diol + NAD+ only L-propane-1,2-diol is oxidized Escherichia coli (S)-lactaldehyde + NADH
-
?
ethanol + NAD+
-
Escherichia coli acetaldehyde + NADH
-
r
ethylene glycol + NAD+
-
Escherichia coli glycolaldehyde + NADH + H+
-
r
glycerol + NAD+
-
Escherichia coli DL-glyceraldehyde + NADH
-
r
propanol + NAD+
-
Escherichia coli propionaldehyde + NADH
-
r

Subunits

Subunits Comment Organism
dimer 2 * 39000, SDS-PAGE Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
reduction of L-lactaldehyde Escherichia coli
9.5
-
dehydrogenation of L-1,2-propanediol Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
4.5 10 reduction of lactaldehyde, pH 4.5: 50% of reductase activity maximum, pH 10: 10% of reductase activity maximum Escherichia coli
8 11 dehydrogenation of 1,2-propanediol, pH 8: 10% of dehydrogenase activity maximum, pH 11: 65% of dehydrogenase activity maximum Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli
NADH
-
Escherichia coli