Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Ralstonia sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-2-hydroxypropiophenone | - |
Ralstonia sp. | |
Cyclohexanol | - |
Ralstonia sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
NADPH | with benzaldehyde as cosubstrate, at pH 7.5 and 30°C | Ralstonia sp. | |
0.008 | - |
NADPH | with cyclohexanone as cosubstrate, at pH 7.5 and 30°C | Ralstonia sp. | |
0.06 | - |
NADP+ | with benzyl alcohol as cosubstrate, at pH 9.0 and 30°C | Ralstonia sp. | |
1.6 | - |
(R)-2-hydroxypropiophenone | at pH 7.5 and 30°C | Ralstonia sp. | |
3.6 | - |
(S)-2-Hydroxypropiophenone | at pH 7.5 and 30°C | Ralstonia sp. | |
5.2 | - |
Cyclohexanol | at pH 9.0 and 30°C | Ralstonia sp. | |
10.8 | - |
cyclohexanone | at pH 7.5 and 30°C | Ralstonia sp. | |
12.3 | - |
benzyl alcohol | at pH 9.0 and 30°C | Ralstonia sp. | |
15.6 | - |
benzaldehyde | at pH 7.5 and 30°C | Ralstonia sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+ has an stabilizing effect. With 1.0 mM CaCl2, the enzyme is completely stable at 0°C for 2 h and after 3.5 h almost 90% of the initial activity is retained | Ralstonia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ralstonia sp. | - |
- |
- |
Ralstonia sp. DSM 6428 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Ralstonia sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
with acetone, at pH 7.5 and 30°C | Ralstonia sp. |
0.05 | - |
with acetaldehyde, at pH 7.5 and 30°C | Ralstonia sp. |
0.08 | - |
with 2,5-hexandione, at pH 7.5 and 30°C | Ralstonia sp. |
0.11 | - |
with cyclohexanone, at pH 7.5 and 30°C | Ralstonia sp. |
0.56 | - |
with 4-phenyl-2-butanone, at pH 7.5 and 30°C | Ralstonia sp. |
4.35 | - |
with acetophenone, at pH 7.5 and 30°C | Ralstonia sp. |
4.89 | - |
with benzaldehyde, at pH 7.5 and 30°C | Ralstonia sp. |
8.36 | - |
with cyclohexanol, at pH 10.5 and 30°C | Ralstonia sp. |
9.78 | - |
with cyclohexanone, at pH 7.5 and 30°C | Ralstonia sp. |
11.58 | - |
with 3-methylcyclohexanol, at pH 10.5 and 30°C | Ralstonia sp. |
11.7 | - |
with 3-fluorobenzaldehyde, at pH 7.5 and 30°C | Ralstonia sp. |
12.62 | - |
with 3-methylcyclohexanone, at pH 7.5 and 30°C | Ralstonia sp. |
17.1 | - |
with (S)-2-hydroxypropiophenone, at pH 7.5 and 30°C | Ralstonia sp. |
18.83 | - |
with cyclohexenol, at pH 10.5 and 30°C | Ralstonia sp. |
23.31 | - |
2-bromobenzaldehyde, at pH 7.5 and 30°C | Ralstonia sp. |
362.6 | - |
with (R)-2-hydroxypropiophenone, at pH 7.5 and 30°C | Ralstonia sp. |
Storage Stability | Organism |
---|---|
-20°C, 10 mM TEA-HCl buffer pH 7.5, at least 80 days, no loss of activity | Ralstonia sp. |
8°C, 50 mM TEA-HCl buffer pH 7.5, 1 day, 91% loss of activity | Ralstonia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1R)-1-hydroxy-1-phenylpropan-2-one + NADPH + H+ | - |
Ralstonia sp. | (1R)-1-phenylpropane-1,2-diol + NADP+ | - |
? | |
(2R)-2-hydroxy-1-(4-methoxyphenyl)propan-1-one + NADPH + H+ | - |
Ralstonia sp. | (2R)-1-(4-methoxyphenyl)propane-1,2-diol + NADP+ | - |
? | |
(2S)-2-hydroxy-1-(4-methoxyphenyl)propan-1-one + NADPH + H+ | - |
Ralstonia sp. | (2S)-1-(4-methoxyphenyl)propane-1,2-diol + NADP+ | - |
? | |
(R)-2-hydroxypropiophenone + NADPH + H+ | - |
Ralstonia sp. | ? | - |
r | |
(S)-2-hydroxypropiophenone + NADPH + H+ | - |
Ralstonia sp. | ? | - |
r | |
2,5-hexandione + NADH + H+ | - |
Ralstonia sp. | ? | - |
r | |
2-bromobenzaldehyde + NADPH | - |
Ralstonia sp. | 2-bromobenzyl alcohol + NADP+ | - |
r | |
2-bromobenzaldehyde + NADPH + H+ | - |
Ralstonia sp. | 2-bromobenzyl alcohol + NADP+ | - |
? | |
2-cyclohexenol + NADP+ | - |
Ralstonia sp. | 2-cyclohexenone + NADH + H+ | - |
r | |
2-cyclohexenone + NADH + H+ | - |
Ralstonia sp. | 2-cyclohexenol + NADP+ | - |
r | |
2-fluorobenzaldehyde + NADPH + H+ | - |
Ralstonia sp. | 2-fluorobenzyl alcohol + NADP+ | - |
? | |
2-hydroxy-2-methyl-1-phenylpropan-1-one + NADPH + H+ | - |
Ralstonia sp. | 2-methyl-1-phenylpropan-1,2-diol + NADP+ | - |
? | |
2-methoxybenzaldehyde + NADPH + H+ | - |
Ralstonia sp. | 2-methoxybenzyl alcohol + NADP+ | - |
? | |
2-methoxybenzaldehyde + NADPH + H+ | - |
Ralstonia sp. DSM 6428 | 2-methoxybenzyl alcohol + NADP+ | - |
? | |
2-methylbenzaldehyde + NADPH + H+ | - |
Ralstonia sp. | 2-methylbenzyl alcohol + NADP+ | - |
? | |
3-fluorobenzaldehyde + NADPH | - |
Ralstonia sp. | 3-fluorobenzyl alcohol + NADP+ | - |
r | |
3-methoxybenzaldehyde + NADPH + H+ | - |
Ralstonia sp. | 3-methoxybenzyl alcohol + NADP+ | - |
? | |
3-methylbenzaldehyde + NADPH + H+ | - |
Ralstonia sp. | 3-methylbenzyl alcohol + NADP+ | - |
? | |
3-methylcyclohexanol + NADP+ | - |
Ralstonia sp. | ? | - |
r | |
3-methylcyclohexanone + NADH + H+ | - |
Ralstonia sp. | 3-methylcyclohexanol + NAD+ | - |
r | |
4-bromobenzaldehyde + NADPH + H+ | - |
Ralstonia sp. | 4-bromobenzyl alcohol + NADP+ | - |
? | |
4-fluorobenzaldehyde + NADPH + H+ | - |
Ralstonia sp. | 4-fluorobenzyl alcohol + NADP+ | - |
? | |
4-methoxybenzaldehyde + NADPH + H+ | - |
Ralstonia sp. | 4-methoxybenzyl alcohol + NADP+ | - |
? | |
4-methoxybenzaldehyde + NADPH + H+ | - |
Ralstonia sp. DSM 6428 | 4-methoxybenzyl alcohol + NADP+ | - |
? | |
4-methylbenzaldehyde + NADPH + H+ | - |
Ralstonia sp. | 4-methylbenzyl alcohol + NADP+ | - |
? | |
4-methylpentan-2-one + NADPH + H+ | - |
Ralstonia sp. | 4-methylpentan-2-ol + NADP+ | - |
? | |
4-phenyl-2-butanone + NADH + H+ | - |
Ralstonia sp. | 4-phenyl-2-butanol + NAD+ | - |
r | |
acetaldehyde + NADPH | - |
Ralstonia sp. | ethanol + NADP+ | - |
r | |
acetone + NADH + H+ | - |
Ralstonia sp. | propan-2-ol + NAD+ | - |
r | |
acetophenone + NADH + H+ | - |
Ralstonia sp. | 1-phenylethan-1-ol + NAD+ | - |
r | |
benzaldehyde + NADPH | - |
Ralstonia sp. | benzyl alcohol + NADP+ | - |
r | |
benzaldehyde + NADPH | - |
Ralstonia sp. DSM 6428 | benzyl alcohol + NADP+ | - |
r | |
benzyl alcohol + NADP+ | - |
Ralstonia sp. | benzaldehyde + NADPH | - |
r | |
benzyl alcohol + NADP+ | - |
Ralstonia sp. DSM 6428 | benzaldehyde + NADPH | - |
r | |
butan-2-ol + NADP+ | - |
Ralstonia sp. | butan-2-one + NADPH + H+ | - |
? | |
butan-2-one + NADPH + H+ | - |
Ralstonia sp. | butan-2-ol + NADP+ | - |
? | |
cyclohexanol + NADP+ | - |
Ralstonia sp. | cyclohexanone + NADH + H+ | - |
r | |
cyclohexanone + NADH + H+ | - |
Ralstonia sp. | cyclohexanol + NAD+ | - |
r | |
cyclohexanone + NADH + H+ | - |
Ralstonia sp. | cyclohexanol + NADP+ | - |
r | |
formaldehyde + NADPH + H+ | - |
Ralstonia sp. | methanol + NADP+ | - |
? | |
furan-2-carbaldehyde + NADPH + H+ | - |
Ralstonia sp. | (furan-2-yl)methanol + NADP+ | - |
? | |
heptan-4-one + NADPH + H+ | - |
Ralstonia sp. | heptan-4-ol + NADP+ | - |
? | |
hexan-1-ol + NADP+ | - |
Ralstonia sp. | hexaldehyde + NADPH + H+ | - |
? | |
hexan-2-one + NADPH + H+ | - |
Ralstonia sp. | hexan-2-ol + NADP+ | - |
? | |
hexanal + NADPH + H+ | - |
Ralstonia sp. | hexan-1-ol + NADP+ | - |
? | |
additional information | no activity with methanol and ethanol | Ralstonia sp. | ? | - |
? | |
additional information | no activity with methanol and ethanol | Ralstonia sp. DSM 6428 | ? | - |
? | |
octan-1-ol + NADP+ | - |
Ralstonia sp. | octaldehyde + NADPH + H+ | - |
? | |
octan-2-one + NADPH + H+ | - |
Ralstonia sp. | octan-2-ol + NADP+ | - |
? | |
pentan-1-ol + NADP+ | - |
Ralstonia sp. | pentaldehyde + NADPH + H+ | - |
? | |
propan-1-ol + NADP+ | - |
Ralstonia sp. | propionaldehyde + NADPH + H+ | - |
? | |
propan-2-ol + NADP+ | - |
Ralstonia sp. | propan-2-one + NADPH + H+ | - |
? | |
pyridine-2-carbaldehyde + NADPH + H+ | - |
Ralstonia sp. | (pyridin-2-yl)methanol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADPH-dependent alcohol dehydrogenase | - |
Ralstonia sp. |
RADH | - |
Ralstonia sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
8 | 25 | the enzyme has a half-life of 80 h at 25°C and stability optimum between 8 and 15°C in the presence of 0.8 mM CaCl2 (half-life of 130 h) | Ralstonia sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | reduction reaction | Ralstonia sp. |
10 | 11.5 | oxidation reaction | Ralstonia sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | the enzyme shows half-lives of 60-70 h between pH 5.5 and 8.0 at room temperature | Ralstonia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Ralstonia sp. | |
NADPH | - |
Ralstonia sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
11.4 | - |
Cyclohexanol | at pH 9.0 and 30°C | Ralstonia sp. | |
16.4 | - |
(R)-2-hydroxypropiophenone | at pH 7.5 and 30°C | Ralstonia sp. |