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Literature summary for 1.1.1.67 extracted from

  • Schafer, A.; Stein, M.A.; Schneider, K.H.; Giffhorn, F.
    Mannitol dehydrogenase from Rhodobacter sphaeroides Si4: subcloning, overexpression in Escherichia coli and characterization of the recombinant enzyme (1997), Appl. Microbiol. Biotechnol., 48, 47-52.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
D-mannitol recombinant protein Cereibacter sphaeroides
1.6
-
D-arabitol recombinant protein Cereibacter sphaeroides
58.5
-
D-sorbitol recombinant protein Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
recombinant protein, SDS-PAGE Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
phototrophic bacterium
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
68.3
-
recombinant protein Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabitol + NAD+
-
Cereibacter sphaeroides ? + NADH
-
?
D-glucitol + NAD+
-
Cereibacter sphaeroides ? + NADH
-
?
D-mannitol + NAD+
-
Cereibacter sphaeroides D-fructose + NADH + H+
-
?
D-sorbitol + NAD+
-
Cereibacter sphaeroides ?
-
r
L-sorbitol + NAD+
-
Cereibacter sphaeroides L-sorbose + NADH + H+
-
?