KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0083 | - |
NADPH | - |
Sus scrofa | |
0.04 | - |
androstenedione | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35500 | - |
- |
Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-estradiol + NADP+ | Rattus norvegicus | - |
estrone + NADPH | - |
r | |
17beta-estradiol + NADP+ | Sus scrofa | - |
estrone + NADPH | - |
r | |
testosterone + NADP+ | Rattus norvegicus | - |
androst-4-ene-3,17-dione + NADPH + H+ | - |
r | |
testosterone + NADP+ | Sus scrofa | - |
androst-4-ene-3,17-dione + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Sephadex G-100, DEAE-cellulose, Bio-Gel P-100 | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0033 | - |
enzyme activity in smooth-surfaced microsomes | Rattus norvegicus |
67.7 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, 6 months, no loss of activity | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-estradiol + NADP+ | - |
Rattus norvegicus | estrone + NADPH | - |
r | |
17beta-estradiol + NADP+ | - |
Sus scrofa | estrone + NADPH | - |
r | |
testosterone + NADP+ | - |
Rattus norvegicus | androst-4-ene-3,17-dione + NADPH + H+ | - |
r | |
testosterone + NADP+ | - |
Sus scrofa | androst-4-ene-3,17-dione + NADPH + H+ | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
in phosphate buffer at pH 7.4 | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | at 37°C | Sus scrofa |
8.5 | - |
at 50°C | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Rattus norvegicus | |
NADP+ | - |
Sus scrofa |