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Literature summary for 1.1.1.64 extracted from
- Purification and properties of a new testosterone 17beta-dehydrogenase (NADP+) from guinea-pig liver (1977), Biochem. J., 163, 401-407.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0067 | - |
17beta-hydroxy-5beta-androstane-3-one | - |
Cavia porcellus |  |
| 0.011 | - |
5beta-androstane-3alpha,17beta-diol | - |
Cavia porcellus | |
| 0.023 | - |
5alpha-androstane-3alpha,17beta-diol | - |
Cavia porcellus | |
| 0.027 | - |
testosterone | - |
Cavia porcellus | |
| 0.029 | - |
5beta-androstane-3beta,17beta-diol | - |
Cavia porcellus | |
| 0.047 | - |
NADP+ | - |
Cavia porcellus | |
| 0.053 | - |
19-nortestosterone | - |
Cavia porcellus | |
| 0.077 | - |
17beta-hydroxy-5alpha-androstane-3-one | - |
Cavia porcellus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
newly discovered isoenzyme, disc electrophoresis | Cavia porcellus |
| 39000 | - |
newly discovered isoenzyme, gel filtration | Cavia porcellus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 17beta-estradiol + NADP+ | Cavia porcellus | - |
estrone + NADPH | - |
r | |
| testosterone + NADP+ | Cavia porcellus | - |
androst-4-ene-3,17-dione + NADPH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, Sephadex G-100, Sephadex G-75, DEAE-cellulose, hydroxyapatite, newly discovered isoenzyme and classical enzyme | Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.647 | - |
newly discovered isoenzyme | Cavia porcellus |
| 2.316 | - |
classical enzymes | Cavia porcellus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, 100 mM sodium phosphate, pH 8.0, 0.05% 2-mercaptoethanol, 1 month, loss of 70% activity | Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 17beta-estradiol + NADP+ | - |
Cavia porcellus | estrone + NADPH | - |
r | |
| 19-nortestosterone + NADP+ | 93% activity compared to testosterone oxidation | Cavia porcellus | 19-norandrostenedione + NADPH | - |
r | |
| 5alpha-androstane-17beta-ol-3-one + NADP+ | 30% activity compared to testosterone oxidation | Cavia porcellus | 5alpha-androstane-3,17-dione + NADPH | - |
r | |
| 5alpha-androstane-3alpha,17beta-diol + NADP+ | 93% activity compared to testosterone oxidation | Cavia porcellus | 5alpha-androstane-3alpha-ol-17-one + NADPH | - |
r | |
| 5alpha-androstane-3alpha,17beta-diol + NADP+ | 63% activity compared to testosterone oxidation | Cavia porcellus | 5alpha-androstane-3alpha-ol-17-one + NADPH | - |
r | |
| 5alpha-androstane-3beta,17beta-diol + NADP+ | 25% activity compared to testosterone oxidation | Cavia porcellus | 5alpha-androstane-3beta-ol-17-one + NADPH | - |
r | |
| 5alpha-androstane-3beta,17beta-diol + NADP+ | 134% activity compared to testosterone oxidation | Cavia porcellus | 5alpha-androstane-3beta-ol-17-one + NADPH | - |
r | |
| 5beta-androstane-17beta-ol + NADP+ | 117% activity compared to testosterone oxidation | Cavia porcellus | 5alpha-androstane-17-one + NADPH | - |
r | |
| 5beta-androstane-17beta-ol-3-one + NADP+ | 330% activity compared to testosterone oxidation | Cavia porcellus | 5beta-androstane-3,17-dione + NADPH | - |
r | |
| 5beta-androstane-3alpha,17beta-diol + NADP+ | 1180% activity compared to testosterone oxidation | Cavia porcellus | 5beta-androstane-3alpha-ol-17-one + NADPH + H+ | - |
r | |
| 5beta-androstane-3beta,17beta-diol + NADP+ | - |
Cavia porcellus | 5alpha-androstane-3beta-ol-17-one + NADPH | - |
r | |
| 5beta-dihydrotestosterone + NADP+ | - |
Cavia porcellus | 5beta-17-oxo-dihydrotestosterone + NADPH | - |
? | |
| testosterone + NADP+ | - |
Cavia porcellus | androst-4-ene-3,17-dione + NADPH + H+ | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 47 | - |
classical enzyme | Cavia porcellus |
| 50 | - |
newly discovered isoenzyme | Cavia porcellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.1 | - |
reduction of androst-4-ene-3,17-dione | Cavia porcellus |
| 10.6 | - |
oxidation of testosterone | Cavia porcellus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Cavia porcellus | |
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