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Literature summary for 1.1.1.62 extracted from

  • Puranen, T.; Poutanen, M.; Ghosh, D.; Vihko, P.; Vihko, R.
    Characterization of structural and functional properties of human 17beta-hydroxysteroid dehydrogenase type 1 using recombinant enzymes and site-directed mutagenesis (1997), Mol. Endocrinol., 11, 77-86.
    View publication on PubMed
Search for more literature for 1.1.1.62

Protein Variants

Protein Variants Comment Organism
A170E/F172E no detectable activity Homo sapiens
K159A almost inactive enzyme, catalytic efficiencies of the substituted enzyme are more than 200fold lower for both reduction and oxidation compared with the values measured for the wild type enzyme Homo sapiens
L111E/V113F no detectable activity Homo sapiens
additional information deleting the last 36 amino acids does not have a dramatic effect on the catalytic properties Homo sapiens
S142A almost inactive enzyme, catalytic efficiencies of the substituted enzyme are more than 200fold lower for both reduction and oxidation compared with the values measured for the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-values for mutant enzymes in cell homogenate Homo sapiens
0.0021
-
 estradiol-17beta wild-type enzyme Homo sapiens
0.00253
-
 estradiol-17beta mutant enzyme SS134A Homo sapiens
0.78
-
 estrone mutant enzyme S134A Homo sapiens
0.9
-
 estrone wilde-type enzyme Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000 78000 non-denaturing PAGE, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
estrone + NADH Homo sapiens type 1 enzyme, catalyzes the conversion of the low activity estrogen, estrone, into highly active estradiol, both in the gonads and in target tissues estradiol-17beta + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 type 1 enzyme
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein enzyme is phosphorylated by protein kinase A exclusively at Ser134 in vitro, but this phosphorylation has no effect on the catalytic properties of the type 1 enzyme, and most likely it does not occur in vivo Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
estradiol-17beta + NAD+
-
 Homo sapiens estrone + NADH
-
 ?
estrone + NADH
-
 Homo sapiens estradiol-17beta + NAD+
-
 ?
estrone + NADH type 1 enzyme, catalyzes the conversion of the low activity estrogen, estrone, into highly active estradiol, both in the gonads and in target tissues Homo sapiens estradiol-17beta + NAD+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+ cofactor Homo sapiens
NADH cofactor Homo sapiens