Literature summary for 1.1.1.61 extracted from

Taxon, E.S.; Halbers, L.P.; Parsons, S.M.
Kinetics aspects of Gamma-hydroxybutyrate dehydrogenase (2020), Biochim. Biophys. Acta Proteins Proteom., 1868, 140376 .

Search for more literature for 1.1.1.61

Activating Compound

Activating Compound	Comment	Organism	Structure
nudix hydrolase	at optimal pH 9.0, the GHBDH reaction is activated about 2fold by saturating purified nudix hydrolases, namely Bacillus methanolicus activator (ACT, UniProt: I3EA59) and Escherichia coli NudF (UniProt Q93K97) proteins. At physiological pH values of about 7.0, ACT activates by more than 3.5-fold	Cupriavidus necator

Crystallization (Commentary)

Crystallization (Comment)	Organism
homology modeling of structure	Cupriavidus necator

Protein Variants

Protein Variants	Comment	Organism
D193A	loss of more than 99% of activity compared to wild-type	Cupriavidus necator
H197A	loss of more than 99% of activity compared to wild-type	Cupriavidus necator
H261A	loss of more than 99% of activity compared to wild-type	Cupriavidus necator
H280A	loss of more than 99% of activity compared to wild-type	Cupriavidus necator

Inhibitors

Inhibitors	Comment	Organism	Structure
NADH	product inhibition, mixed type inhibition	Cupriavidus necator
succinate semialdehyde	product inhibition, mixed type inhibition with respect to NAD+, competititve with respect to 4-hydroxybutanoate	Cupriavidus necator

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.045	-	NAD+	pH 9.0, 23°C	Cupriavidus necator
0.52	-	4-hydroxybutanoate	pH 9.0, 23°C	Cupriavidus necator

Organism

Organism	UniProt	Comment	Textmining
Cupriavidus necator	Q59104	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-hydroxybutanoate + NAD+	-	Cupriavidus necator	succinate semialdehyde + NADH + H+	-	?

Synonyms

Synonyms	Comment	Organism
gbd	-	Cupriavidus necator
GHBDH	-	Cupriavidus necator
NAD-dependent 4-hydroxybutyrate dehydrogenase	-	Cupriavidus necator

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Cupriavidus necator

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0084	-	succinate semialdehyde	substrate 4-hydroxybutanoate, pH 9, 23°C	Cupriavidus necator
0.0118	-	succinate semialdehyde	substrate NAD+, pH 9, 23°C	Cupriavidus necator
0.0236	-	NADH	substrate NAD+, pH 9, 23°C	Cupriavidus necator
0.0933	-	NADH	substrate 4-hydroxybutanoate, pH 9, 23°C	Cupriavidus necator

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Release 2023.1 (January 2023)