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Literature summary for 1.1.1.6 extracted from

  • Chauliac, D.; Wang, Q.; St John, F.J.; Jones, G.; Hurlbert, J.C.; Ingram, L.O.; Shanmugam, K.T.
    Kinetic characterization and structure analysis of an altered polyol dehydrogenase with D-lactate dehydrogenase activity (2020), Protein Sci., 29, 2387-2397 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of mutant D121N/F245S Weizmannia coagulans

Protein Variants

Protein Variants Comment Organism
D121N mutation lowers the activity of the enzyme with most all the tested substrates relative to the native enzyme Weizmannia coagulans
D121N/F245S mutant acquires D-lactate dehydrogenase activiy, the alteration increases the capacity of the glycerol binding site and facilitated hydrogen bonding between the S245 gamma-O and the C1 carboxylate of pyruvate Weizmannia coagulans
F245S mutant acquires D-lactate dehydrogenase activiy, the alteration increases the capacity of the glycerol binding site and facilitated hydrogen bonding between the S245 gamma-O and the C1 carboxylate of pyruvate Weizmannia coagulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
NADH wild-type, pH 5.4, 55°C Weizmannia coagulans
0.1
-
NADH mutant D121N, pH 5.4, 55°C Weizmannia coagulans
0.1
-
NADH mutant F245S, pH 5.4, 55°C Weizmannia coagulans
0.3
-
NADH mutant /D121NF245S, pH 5.9, 55°C Weizmannia coagulans
0.4
-
NAD+ wild-type, pH 8.9, 55°C Weizmannia coagulans
0.7
-
NAD+ mutant F245S, pH 8.9, 55°C Weizmannia coagulans
1
-
D-1,2-propanediol wild-type, pH 8.9, 55°C Weizmannia coagulans
1.5
-
NAD+ mutant D121N, pH 8.9, 55°C Weizmannia coagulans
2.3
-
NAD+ mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
3
-
1,2-Butanediol wild-type, pH 8.9, 55°C Weizmannia coagulans
4
-
1,2-Butanediol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
4
-
glycerol wild-type, pH 8.9, 55°C Weizmannia coagulans
4.5
-
D-1,2-propanediol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
6.5
-
D-2,3-butanediol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
8
-
D-1,2-propanediol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
17
-
D-2,3-butanediol wild-type, pH 8.9, 55°C Weizmannia coagulans
19
-
D-1,2-propanediol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
23.5
-
1,2-Butanediol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
39
-
D-2,3-butanediol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
41
-
glycerol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
44
-
1,2-Butanediol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
54.5
-
pyruvate mutant F245S, pH 5.4, 55°C Weizmannia coagulans
55.5
-
pyruvate mutant /D121NF245S, pH 5.9, 55°C Weizmannia coagulans
60.5
-
pyruvate mutant D121N, pH 5.4, 55°C Weizmannia coagulans
90
-
D-2,3-butanediol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
148
-
glycerol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
195
-
glycerol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
207
-
pyruvate wild-type, pH 5.4, 55°C Weizmannia coagulans

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 1.9fold increase in activity in presence of Zn2+ Weizmannia coagulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
gel filtration Weizmannia coagulans

Organism

Organism UniProt Comment Textmining
Weizmannia coagulans A0A150JSL8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-butanediol + NAD+
-
Weizmannia coagulans 1-hydroxy-2-butanone + NADH + H+
-
?
1,2-ethanediol + NAD+
-
Weizmannia coagulans hydroxyacetaldehyde + NADH + H+
-
?
1,3-butanediol + NAD+
-
Weizmannia coagulans 4-hydroxy-2-butanone + NADH + H+
-
?
1,4-butanediol + NAD+
-
Weizmannia coagulans ? + NADH + H+
-
?
D-1,2-propanediol + NAD+
-
Weizmannia coagulans hydroxyacetone + NADH + H+
-
?
D-2,3-butanediol + NAD+
-
Weizmannia coagulans ? + NADH + H+
-
?
glycerol + NAD+
-
Weizmannia coagulans glycerone + NADH + H+
-
?
L-1,2-propanediol + NAD+
-
Weizmannia coagulans hydroxyacetone + NADH + H+
-
?
L-2,3-butanediol + NAD+
-
Weizmannia coagulans ? + NADH + H+
-
?
pyruvate + NADH + H+
-
Weizmannia coagulans D-lactate + NADH
-
?

Subunits

Subunits Comment Organism
dimer 2 * 39000, calculated from sequence Weizmannia coagulans

Synonyms

Synonyms Comment Organism
B4100_2156
-
Weizmannia coagulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 65 mutant F245S, pH 5, reduction of pyruvate Weizmannia coagulans
60
-
mutant D121N/F245S Weizmannia coagulans
65 75 wild-type Weizmannia coagulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
1 h, 95% loss of activity Weizmannia coagulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
pyruvate mutant D121N, pH 5.4, 55°C Weizmannia coagulans
10.5
-
D-2,3-butanediol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
10.5
-
pyruvate wild-type, pH 5.4, 55°C Weizmannia coagulans
11
-
glycerol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
29
-
D-1,2-propanediol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
42.5
-
D-1,2-propanediol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
42.5
-
glycerol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
50
-
1,2-Butanediol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
50
-
glycerol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
50.5
-
D-2,3-butanediol wild-type, pH 8.9, 55°C Weizmannia coagulans
57.5
-
D-2,3-butanediol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
66.5
-
1,2-Butanediol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
71
-
1,2-Butanediol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
87.5
-
D-1,2-propanediol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
92
-
D-1,2-propanediol wild-type, pH 8.9, 55°C Weizmannia coagulans
117.5
-
glycerol wild-type, pH 8.9, 55°C Weizmannia coagulans
120.5
-
1,2-Butanediol wild-type, pH 8.9, 55°C Weizmannia coagulans
136
-
D-2,3-butanediol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
165
-
pyruvate mutant /D121NF245S, pH 5.9, 55°C Weizmannia coagulans
352
-
pyruvate mutant F245S, pH 5.4, 55°C Weizmannia coagulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
mutant F245S, reduction of pyruvate Weizmannia coagulans
9
-
wild-type Weizmannia coagulans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.02
-
pyruvate mutant D121N, pH 5.4, 55°C Weizmannia coagulans
0.05
-
pyruvate wild-type, pH 5.4, 55°C Weizmannia coagulans
0.1
-
glycerol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
0.1
-
D-2,3-butanediol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
0.3
-
glycerol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
1
-
1,2-Butanediol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
1
-
glycerol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
1.5
-
D-2,3-butanediol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
1.5
-
D-1,2-propanediol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
3
-
pyruvate mutant /D121NF245S, pH 5.9, 55°C Weizmannia coagulans
3
-
1,2-Butanediol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
3
-
D-2,3-butanediol wild-type, pH 8.9, 55°C Weizmannia coagulans
5.5
-
D-1,2-propanediol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
6.5
-
pyruvate mutant F245S, pH 5.4, 55°C Weizmannia coagulans
15.5
-
1,2-Butanediol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
20
-
D-1,2-propanediol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
21
-
D-2,3-butanediol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
30
-
glycerol wild-type, pH 8.9, 55°C Weizmannia coagulans
40
-
1,2-Butanediol wild-type, pH 8.9, 55°C Weizmannia coagulans
92
-
D-1,2-propanediol wild-type, pH 8.9, 55°C Weizmannia coagulans