General Stability | Organism |
---|---|
manganese substitution increases the half-life of GDH by 6folds at 60°C and 70°C | Klebsiella pneumoniae subsp. pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic and thermodynamic analysis, Michaelis-Menten kinetics, overview | Klebsiella pneumoniae subsp. pneumoniae | |
0.23 | - |
NAD+ | Mg-GDH, pH 12.0, 45°C, recombinant enzyme | Klebsiella pneumoniae subsp. pneumoniae | |
0.38 | - |
NAD+ | wild-type Zn-GDH, pH 12.0, 45°C, recombinant enzyme | Klebsiella pneumoniae subsp. pneumoniae | |
1.12 | - |
NAD+ | Mg-GDH, pH 12.0, 45°C, recombinant enzyme | Klebsiella pneumoniae subsp. pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | improvement of stability, activity, and substrate promiscuity of glycerol dehydrogenase substituted by divalent metal ions Mn2+ and Mg2+, overview. The activity of Mn-GDH and Mg-GDH improves several folds in comparison to the native GDH. The activity of substituted GDH towards non-natural substrates, 4-chloroacetoacetate, 3-chloroacetylpyridine, p-chloroacetophenone, and acetophenone is 30folds higher than native GDH. Manganese substitution increases the half-life of GDH by 6folds at 60°C and 70°C | Klebsiella pneumoniae subsp. pneumoniae | |
Mn2+ | improvement of stability, activity, and substrate promiscuity of glycerol dehydrogenase substituted by divalent metal ions Mn2+ and Mg2+, overview | Klebsiella pneumoniae subsp. pneumoniae | |
additional information | bifunctional role of metal ions in GDH in catalysis and structure stabilization | Klebsiella pneumoniae subsp. pneumoniae | |
Zn2+ | glycerol dehydrogenase from Klebsiella pneumoniae is a zinc-dependent metalloenzyme | Klebsiella pneumoniae subsp. pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | Klebsiella pneumoniae subsp. pneumoniae | - |
glycerone + NADH + H+ | - |
r | |
glycerol + NAD+ | Klebsiella pneumoniae subsp. pneumoniae ATCC 700721 | - |
glycerone + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae subsp. pneumoniae | A6TGD6 | - |
- |
Klebsiella pneumoniae subsp. pneumoniae ATCC 700721 | A6TGD6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | - |
Klebsiella pneumoniae subsp. pneumoniae | glycerone + NADH + H+ | - |
r | |
glycerol + NAD+ | - |
Klebsiella pneumoniae subsp. pneumoniae ATCC 700721 | glycerone + NADH + H+ | - |
r | |
additional information | the enzyme is also active with substrates 4-chloroacetoacetate, 3-chloroacetylpyridine, 4-chloroacetophenone, and acetophenone, substrate specificities of enzyme with bound Zn2+, Mn2+, or Mg2+, overview | Klebsiella pneumoniae subsp. pneumoniae | ? | - |
? | |
additional information | the enzyme is also active with substrates 4-chloroacetoacetate, 3-chloroacetylpyridine, 4-chloroacetophenone, and acetophenone, substrate specificities of enzyme with bound Zn2+, Mn2+, or Mg2+, overview | Klebsiella pneumoniae subsp. pneumoniae ATCC 700721 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Klebsiella pneumoniae subsp. pneumoniae |
GldA | - |
Klebsiella pneumoniae subsp. pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
assay at | Klebsiella pneumoniae subsp. pneumoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal inactivation of GDH is not a simple first-order process, thermal deactivation kinetics, modelling, overview | Klebsiella pneumoniae subsp. pneumoniae |
30 | 50 | purified recombinant enzyme, pH 12.0, quite stable over 150 min | Klebsiella pneumoniae subsp. pneumoniae |
60 | - |
purified recombinant enzyme, pH 12.0, loss of about 25% activity after 100 min | Klebsiella pneumoniae subsp. pneumoniae |
70 | - |
purified recombinant enzyme, pH 12.0, loss of about 75% activity after 80 min | Klebsiella pneumoniae subsp. pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
12 | - |
assay at | Klebsiella pneumoniae subsp. pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Klebsiella pneumoniae subsp. pneumoniae | |
NADH | - |
Klebsiella pneumoniae subsp. pneumoniae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
46.1 | - |
NAD+ | wild-type Zn-GDH, pH 12.0, 45°C, recombinant enzyme | Klebsiella pneumoniae subsp. pneumoniae | |
83.6 | - |
NAD+ | Mg-GDH, pH 12.0, 45°C, recombinant enzyme | Klebsiella pneumoniae subsp. pneumoniae | |
120.3 | - |
NAD+ | Mg-GDH, pH 12.0, 45°C, recombinant enzyme | Klebsiella pneumoniae subsp. pneumoniae |