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Literature summary for 1.1.1.6 extracted from

  • Leichus, B.N.; Blanchard, J.S.
    Isotopic analysis of the reaction catalyzed by glycerol dehydrogenase (1994), Biochemistry, 33, 14642-14649.
    View publication on PubMed
Search for more literature for 1.1.1.6

Inhibitors

Inhibitors Comment Organism Structure
1,3-Propanediol competitive inhibitor versus glycerol in direction of glycerol oxidation, noncompetitive inhibitor versus NAD+, competitive inhibitor versus dihydroxyacetone in direction of glycerol reduction, noncompetitive inhibitor versus NADH Cellulomonas sp.
NADH competitive inhibitor versus NAD+ in direction of glycerol oxidation, noncompetitive inhibitor versus glycerol Cellulomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 1,2-propanediol pH 8.8, 25°C Cellulomonas sp.
4
-
 3-mercapto-1,2-propanediol pH 8.8, 25°C Cellulomonas sp.
4.4
-
 R-1-amino-2-propanol pH 8.8, 25°C Cellulomonas sp.
5.1
-
 glycerol pH 8.8, 25°C Cellulomonas sp.
6
-
 3-Chloro-1,2-propanediol pH 8.8, 25°C Cellulomonas sp.
6.1
-
 3-bromo-1,2-propanediol pH 8.8, 25°C Cellulomonas sp.
180
-
 3-amino-1,2-propanediol pH 8.8, 25°C Cellulomonas sp.
500
-
 S-1-amino-2-propanol pH 8.8, 25°C Cellulomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm soluble, isolation from crude cell extract Cellulomonas sp. 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required for enzymatic activity and glycerol binding Cellulomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39500
-
 8 * 39500, SDS-PAGE Cellulomonas sp.
324000
-
 gel filtration Cellulomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol + NAD+ Cellulomonas sp.
-
 dihydroxyacetone + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Cellulomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cellulomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-propanediol + NAD+ 105% of the activity with glycerol Cellulomonas sp. hydroxyacetone + NADH
-
 ?
3-amino-1,2-propanediol + NAD+ 104% of the activity with glycerol Cellulomonas sp. ?
-
 ?
3-bromo-1,2-propanediol + NAD+ 109% of the activity with glycerol Cellulomonas sp. ?
-
 ?
3-chloro-1,2-propanediol + NAD+ 130% of the activity with glycerol Cellulomonas sp. ?
-
 ?
3-mercapto-1,2-propanediol + NAD+ 155% of the activity with glycerol Cellulomonas sp. ?
-
 ?
glycerol + NAD+
-
 Cellulomonas sp. dihydroxyacetone + NADH + H+
-
 ?
glycerol + NAD+
-
 Cellulomonas sp. dihydroxyacetone + NADH + H+
-
 r
R-1-amino-2-propanol + NAD+ 33% of the activity with glycerol Cellulomonas sp. ?
-
 ?
S-1-amino-2-propanol + NAD+ 9% of the activity with glycerol Cellulomonas sp. ?
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 39500, SDS-PAGE Cellulomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Cellulomonas sp.
NADH
-
 Cellulomonas sp.