Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.6 extracted from

  • McGregor, W.G.; Philips, J.; Suelter, C.H.
    Purification and kinetic characterization of a monovalent cation-activated glycerol dehydrogenase from Aerobacter aerogenes (1974), J. Biol. Chem., 249, 3132-3139.
    View publication on PubMed
Search for more literature for 1.1.1.6

Activating Compound

Activating Compound Comment Organism Structure
1,10-phenanthroline at 1.85 mM 135% of the activity without activator Klebsiella aerogenes
2-mercaptoethanol 1 mM, slight activation Klebsiella aerogenes
8-Quinolinol 1 mM, slight activation Klebsiella aerogenes
dithioerythritol 1 mM, slight activation Klebsiella aerogenes
NH4+
-
 Klebsiella aerogenes

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol strongly inhibitory at 100 mM, but slight activation in lower concentration, 1 mM Klebsiella aerogenes
8-Quinolinol 1 mM, 10 mM Klebsiella aerogenes
dithioerythritol 10 mM Klebsiella aerogenes
EDTA 3 mM, 30 mM Klebsiella aerogenes
iodoacetamide strong inhibition, 10 mM Klebsiella aerogenes
N-ethylmaleimide
-
 Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
74.3
-
 glycerol in the presence of NH4+ Klebsiella aerogenes

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm soluble, isolation from crude cell extract Klebsiella aerogenes 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates Klebsiella aerogenes
Rb+ activates Klebsiella aerogenes
Tl+ activates Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-
Klebsiella aerogenes 1033
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35
-
-
 Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerol + NAD+
-
 Klebsiella aerogenes dihydroxyacetone + NADH + H+
-
 ?
glycerol + NAD+
-
 Klebsiella aerogenes 1033 dihydroxyacetone + NADH + H+
-
 ?