Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | oxidation and reduction greatly increased | Cellulomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM | Cellulomonas sp. | |
Cd2+ | strongly inhibitory | Cellulomonas sp. | |
Cu2+ | strong inhibition | Cellulomonas sp. | |
dihydroxyacetone | above 0.5 mM | Cellulomonas sp. | |
Fe3+ | - |
Cellulomonas sp. | |
K+ | reduction of dihydroxyacetone | Cellulomonas sp. | |
p-chloromercuribenzoate | - |
Cellulomonas sp. | |
sodium tetraborate | 1 mM, 53% inhibition | Cellulomonas sp. | |
Zn2+ | strong inhibitory | Cellulomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.089 | - |
NAD+ | pH 9.0, 25°C | Cellulomonas sp. | |
0.385 | - |
dihydroxyacetone | pH 6.0, 25°C | Cellulomonas sp. | |
10.9 | - |
glycerol | pH 9.0, 25°C | Cellulomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | soluble, isolation from crude cell extract | Cellulomonas sp. | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | oxidation of glycerol os greatly increased | Cellulomonas sp. | |
Li+ | oxidation slightly increased | Cellulomonas sp. | |
Na+ | oxidation and reduction slightly increased | Cellulomonas sp. | |
Rb+ | oxidation greatly increased | Cellulomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | Cellulomonas sp. | - |
dihydroxyacetone + NADH + H+ | - |
? | |
glycerol + NAD+ | Cellulomonas sp. NT3060 | - |
dihydroxyacetone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas sp. | - |
- |
- |
Cellulomonas sp. NT3060 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cellulomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
138 | - |
- |
Cellulomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-propanediol + NAD+ | relative activity is 116% compared to oxidation of glycerol | Cellulomonas sp. | hydroxyacetone + NADH | - |
? | |
1,2-propanediol + NAD+ | relative activity is 116% compared to oxidation of glycerol | Cellulomonas sp. NT3060 | hydroxyacetone + NADH | - |
? | |
1,3-butanediol + NAD+ | relative activity is 6.5% compared to oxidation of glycerol | Cellulomonas sp. | ? | - |
? | |
1,3-propanediol + NAD+ | relative rate of oxidation is 18% compared to oxidation of glycerol | Cellulomonas sp. | ? | - |
? | |
1,4-butanediol + NAD+ | relative activity is 0.3% compared to oxidation of glycerol | Cellulomonas sp. | ? | - |
? | |
2,3-butanediol + NAD+ | - |
Cellulomonas sp. | 3-hydroxybutane-2-one + NADH | - |
? | |
2,3-butanediol + NAD+ | - |
Cellulomonas sp. NT3060 | 3-hydroxybutane-2-one + NADH | - |
? | |
DL-alpha-glycerophosphate + NAD+ | oxidation at 16% compared to oxidation of glycerol | Cellulomonas sp. | ? | - |
? | |
DL-glyceraldehyde + NAD+ | - |
Cellulomonas sp. | 3-hydroxypyruvaldehyde + NADH | - |
? | |
ethanediol + NAD+ | - |
Cellulomonas sp. | glycolaldehyde + NADH | reduction at concentration of 50 mM | r | |
ethanediol + NAD+ | - |
Cellulomonas sp. NT3060 | glycolaldehyde + NADH | reduction at concentration of 50 mM | r | |
ethylene glycol + NAD+ | - |
Cellulomonas sp. | ? | - |
? | |
glycerol + NAD+ | - |
Cellulomonas sp. | dihydroxyacetone + NADH + H+ | - |
? | |
glycerol + NAD+ | - |
Cellulomonas sp. | dihydroxyacetone + NADH + H+ | - |
r | |
glycerol + NAD+ | - |
Cellulomonas sp. NT3060 | dihydroxyacetone + NADH + H+ | - |
? | |
glycerol + NAD+ | - |
Cellulomonas sp. NT3060 | dihydroxyacetone + NADH + H+ | - |
r | |
glycerol-alpha-monochlorohydrin + NAD+ | relative rate of oxidation is 71% compared to oxidation of glycerol | Cellulomonas sp. | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
oxidation of glycerol | Cellulomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
60 min, stable in the pH range pH 5.5-10 | Cellulomonas sp. |
50 | - |
15 min, stable around pH 7.0 | Cellulomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reaction of dihydroxyacetone | Cellulomonas sp. |
9 | - |
oxidation of glycerol | Cellulomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.8 | 7.8 | pH 4.8: about 40% of maximal activity, pH 7.8: about 50% of maximal activity, reduction of dihydroxyacetone | Cellulomonas sp. |
7 | 10 | pH 7.0: about 60% of maximal activity, pH 10.0: about 85% of maximal activity, oxidation of glycerol | Cellulomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | 30°C, 60 min, stable | Cellulomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Cellulomonas sp. | |
NADH | - |
Cellulomonas sp. |