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Literature summary for 1.1.1.57 extracted from
- D-Mannonate and D-altronate-NAD dehydrogenases from Escherichia coli (1982), Methods Enzymol., 89, 210-218.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | product inhibition with all products in forward and reverse reaction | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
D-fructuronate | - |
Escherichia coli |  |
| 0.78 | - |
D-mannonate | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannonate + NAD+ | Escherichia coli | - |
D-fructuronate + NADH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6 | - |
crude extract | Escherichia coli |
| 320 | - |
purified enzyme | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 10 mM sodium phosphate buffer, pH 7, no loss of activity of the purified enzyme within 3 weeks | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-altronate + NAD+ | D-tagaturonate and D-altronate are 20% as active as D-fructuronate and D-mannonate | Escherichia coli | D-tagaturonate + NADH | - |
r | |
| D-mannonate + NAD+ | - |
Escherichia coli | D-fructuronate + NADH | - |
r | |
| D-mannonate + NADP+ | 5% of activity compared with NAD+ as cofactor | Escherichia coli | D-fructuronate + NADPH | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 53 | - |
50% loss of activity within 10 min | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
for NADH oxidation | Escherichia coli |
| 8.4 | - |
for NAD reduction | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | 5% of activity wit NAD+ | Escherichia coli | |
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Release 2023.1 (January 2023)
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