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Literature summary for 1.1.1.56 extracted from

  • Kahle, C.; Schneider, K.H.; Giffhorn, F.
    Pentitol metabolism of Rodobacter sphaeroides Si4: purification and characterization of a ribitol dehydrogenase (1992), J. Gen. Microbiol., 138, 1277-1281.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.077
-
NAD+ pH 9, 30°C Cereibacter sphaeroides
1.1
-
D-ribulose pH 9, 30°C Cereibacter sphaeroides
6.3
-
ribitol pH 9, 30°C Cereibacter sphaeroides
12.5
-
D-xylulose pH 9, 30°C Cereibacter sphaeroides
77
-
xylitol pH 9, 30°C Cereibacter sphaeroides

Metals/Ions

Metals/Ions Comment Organism Structure
additional information
-
Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
2 * 25000, SDS-PAGE Cereibacter sphaeroides
50000
-
gel filtration Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
59
-
-
Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabitol + NAD+
-
Cereibacter sphaeroides D-xylulose + NADH + H+
-
?
D-glucitol + NAD+
-
Cereibacter sphaeroides ? + NADH
-
?
erythritol + NAD+
-
Cereibacter sphaeroides ? + NADH
-
?
ribitol + NAD+
-
Cereibacter sphaeroides D-ribulose + NADH
-
?
xylitol + NAD+
-
Cereibacter sphaeroides D-xylulose + NADH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 25000, SDS-PAGE Cereibacter sphaeroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Cereibacter sphaeroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 60 50% enzyme activity loss after 1 min to 15 days Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
sugar reduction Cereibacter sphaeroides
9 10.3 alcohol oxidation Cereibacter sphaeroides

pH Range

pH Minimum pH Maximum Comment Organism
8
-
50% of maximal activity Cereibacter sphaeroides
10.7
-
50% of maximal activity Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Cereibacter sphaeroides
NADH
-
Cereibacter sphaeroides