General Stability | Organism |
---|---|
freezing and thawing causes 30-40% decrease of mutant B enzyme activity | Klebsiella aerogenes |
freezing and thawing causes 5-8% decrease of wild type enzyme activity | Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
ribitol | pH 7 | Klebsiella aerogenes | |
147 | - |
L-arabitol | pH 7 | Klebsiella aerogenes | |
579 | - |
xylitol | pH 7 | Klebsiella aerogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
4 * 25000, wild type enzyme, SDS-PAGE | Klebsiella aerogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribitol + NAD+ | Klebsiella aerogenes | - |
D-ribulose + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
inducible enzyme | - |
Klebsiella aerogenes | - |
mutants with constitutive enzyme | - |
Purification (Comment) | Organism |
---|---|
ribitol dehydrogenase B | Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
43.6 | - |
ribitol dehydrogenase B | Klebsiella aerogenes |
Storage Stability | Organism |
---|---|
mutant B enzyme inactive after freezing for 2 months, half life of 10 days at 2°C | Klebsiella aerogenes |
wild type enzyme can be stored frozen for months without greater loss of activity , half life of 70 days at 2°C | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabitol + NAD+ | - |
Klebsiella aerogenes | L-xylulose + NADH + H+ | - |
? | |
ribitol + NAD+ | - |
Klebsiella aerogenes | D-ribulose + NADH | - |
? | |
xylitol + NAD+ | mutants have increased xylitol activity | Klebsiella aerogenes | D-xylulose + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | monomers of 27000, mutant B, SDS-PAGE | Klebsiella aerogenes |
tetramer | 4 * 25000, wild type enzyme, SDS-PAGE | Klebsiella aerogenes |
Synonyms | Comment | Organism |
---|---|---|
ribitol dehydrogenase B | mutant enzyme with different properties | Klebsiella aerogenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
28 | - |
L-arabitol | - |
Klebsiella aerogenes | |
36 | - |
xylitol | - |
Klebsiella aerogenes | |
299 | - |
ribitol | - |
Klebsiella aerogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Klebsiella aerogenes | |
NADH | - |
Klebsiella aerogenes |