Literature summary for 1.1.1.53 extracted from

Carrea, G.; Cremonesi, P.
Enzyme-catalyzed steroid transformations in water-organic solvent two-phase systems (1987), Methods Enzymol., 136, 150-157.

No PubMed abstract available

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.007	-	NADH	-	Streptomyces exfoliatus
0.042	0.048	cortisone	free enzyme	Streptomyces exfoliatus
0.085	0.098	cortisone	immobilized enzyme	Streptomyces exfoliatus

Organism

Organism	UniProt	Comment	Textmining
Streptomyces exfoliatus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Streptomyces exfoliatus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cortisone + NAD(P)H	i.e. 17beta,21-dihydroxy-pregn-4-ene-3,11,20-trione	Streptomyces exfoliatus	17alpha,20beta,21-trihydroxy-pregn-4-ene-3,11-dione + NAD(P)+	-	?
progesterone + NAD(P)H	-	Streptomyces exfoliatus	20beta-hydroxy-pregn-4-ene-3-one + NAD(P)+	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.333	-	cortisone	NADH	Streptomyces exfoliatus
6.67	-	progesterone	NADH	Streptomyces exfoliatus

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Release 2023.1 (January 2023)