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Literature summary for 1.1.1.51 extracted from

  • Fernández-Cabezón, L.; Galán, B.; García, J.
    Engineering Mycobacterium smegmatis for testosterone production (2017), Microb. Biotechnol., 10, 151-161 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.51

Application

Application Comment Organism
synthesis engineering Mycobacterium smegmatis for testosterone production. Mycobacterium smegmatis is an excellent chassis to develop biotechnological processes for the biotransformation of sterols and their derivatives into valuable pharmaceutical compounds. Overexpression of the gene encoding microbial 17beta-hydroxysteroid: NADP 17-oxidoreductase, from the bacterium Comamonas testosteroni. The host strains are Mycobacterium smegmatis wild type and a genetic engineered androst-4-ene-3,17-dione producing mutant. The recombinant strains are able to produce testosterone from androst-4-ene-3,17-dione and/or from sterols with high yields Comamonas testosteroni
synthesis engineering Mycobacterium smegmatis for testosterone production. Mycobacterium smegmatis is an excellent chassis to develop biotechnological processes for the biotransformation of sterols and their derivatives into valuable pharmaceutical compounds. Overexpression of the gene encoding microbial 17beta-hydroxysteroid: NADP 17-oxidoreductase, from the fungus Cochliobolus lunatus. The host strains are Mycobacterium smegmatis wild type and a genetic engineered androst-4-ene-3,17-dione producing mutant. The recombinant strains are able to produce testosterone from androst-4-ene-3,17-dione and/or from sterols with high yields Curvularia lunata

Cloned(Commentary)

Cloned (Comment) Organism
-
 Curvularia lunata
-
 Comamonas testosteroni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
androst-4-ene-3,17-dione + NADPH + H+ Curvularia lunata
-
 testosterone + NADP+
-
 ?
androst-4-ene-3,17-dione + NADPH + H+ Comamonas testosteroni
-
 testosterone + NADP+
-
 ?
androst-4-ene-3,17-dione + NADPH + H+ Comamonas testosteroni ATCC 11996
-
 testosterone + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni P19871
-
-
Comamonas testosteroni ATCC 11996 P19871
-
-
Curvularia lunata
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
androst-4-ene-3,17-dione + NADPH + H+
-
 Curvularia lunata testosterone + NADP+
-
 ?
androst-4-ene-3,17-dione + NADPH + H+
-
 Comamonas testosteroni testosterone + NADP+
-
 ?
androst-4-ene-3,17-dione + NADPH + H+
-
 Comamonas testosteroni ATCC 11996 testosterone + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
17beta-HSD
-
 Curvularia lunata
17beta-hydroxysteroid: NADP 17-oxidoreductase
-
 Curvularia lunata
17beta-hydroxysteroid: NADP 17-oxidoreductase
-
 Comamonas testosteroni
3beta/17beta-HSD
-
 Comamonas testosteroni