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Literature summary for 1.1.1.51 extracted from

  • Li, M.; Xiong, G.; Maser, E.
    A novel transcriptional repressor PhaR for the steroid-inducible expression of the 3,17beta-hydroxysteroid dehydrogenase gene in Comamonas testosteroni ATCC11996 (2013), Chem.-Biol. Interact., 202, 116-125.
    View publication on PubMed

Application

Application Comment Organism
environmental protection transcriptional repressor phaR knockout mutants have better ability to degrade steroids than wild-type Comamonas testosteroni ATCC11996 and might therefore be used in bioremediation Comamonas testosteroni

Cloned(Commentary)

Cloned (Comment) Organism
gene betahsd, cloning of PhaR and 3,17beta-HSD together with their promoter domains into plasmids pK18 and pUC19 and coexpression in Escherichia coli strain HB101. A 2509 bp DNA fragment, that contains a putative promoter for the bhsd gene (without the phaR gene), is cloned into plasmid pUC2.5-3. The plasmid is transformed into strain HB101 and induced with testosterone. 3,17beta-HSD expression results at a high level, but cannot be further enhanced by testosterone. Recombinant expression of His-tagged enzyme in Escherichia coli strain BL21. Promoter cloning Comamonas testosteroni

Protein Variants

Protein Variants Comment Organism
additional information construction of transcriptional repressor PhaR knockout mutants M6 of Comamonas testosteroni, the mutants grow better than wild-type Comamonas testosteroni in the presence of 1 mM testosterone, 0.5 mM estradiol or 0.5 mM cholesterol. phaR Knockout mutants M6 cells are inhibited by estradiol and cholesterol, this inhibition is stronger in M6 cells than in wild-type Comamonas testosteroni Comamonas testosteroni

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni P19871
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-
Comamonas testosteroni ATCC 119960 P19871
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-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography Comamonas testosteroni

Synonyms

Synonyms Comment Organism
3,17beta-HSD
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Comamonas testosteroni
3,17beta-hydroxysteroid dehydrogenase
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Comamonas testosteroni
betahsd
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Comamonas testosteroni

Expression

Organism Comment Expression
Comamonas testosteroni the transcriptional repressor phaR gene (phaR) locates 2290 bp upstream of the betahsd gene and might bind on a special bhsd promoter domain down
Comamonas testosteroni estradiol and cholesterol both cannot induce betahsd gene expression in both wild-type and mutant Comamonas testosteroni additional information
Comamonas testosteroni the enzyme expression is induced by testosterone up

General Information

General Information Comment Organism
evolution 3,17beta-hydroxysteroid dehydrogenase is a member of the short-chain dehydrogenase/reductase (SDR) superfamily Comamonas testosteroni
additional information PhaR is a repressor that controls 3,17beta-HSD expression, phaR knock-out mutants grow at higher rates and produce more protein in the presence of steroids as carbon source. PhaR also regulates other genes that relate to steroid degradation. Estradiol and cholesterol both cannot induce betahsd gene expression in both wild-type and mutant Comamonas testosteroni Comamonas testosteroni
physiological function 3,17beta-hydroxysteroid dehydrogenase is a key enzyme in steroid degradation Comamonas testosteroni