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Literature summary for 1.1.1.51 extracted from

  • Ulrih, N.P.; Lanisnik Rizner, T.
    Conformational stability of 17 beta-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus (2006), FEBS J., 273, 3927-3937.
    View publication on PubMed

General Stability

General Stability Organism
unfolding through 0.4 M guanidine hydrochloride Curvularia lunata
unfolding through 1.2 M urea Curvularia lunata

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl addition of a neutral electrolyte, such as NaCl, shields repulsion interactions in the highly positively charged enzyme at pH 2.0, effects on the secondary structure by increasing NaCl concentrations at pH 2.0 and 25°C, overview Curvularia lunata

Organism

Organism UniProt Comment Textmining
Curvularia lunata
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-estrene-17beta-ol-3-one + NADP+
-
Curvularia lunata 4-estrene-3,17-dione + NADPH
-
r

Subunits

Subunits Comment Organism
dimer secondary structure analysis at 25°C and different pH values Curvularia lunata

Synonyms

Synonyms Comment Organism
17 beta-hydroxysteroid dehydrogenase
-
Curvularia lunata
17beta-HSD
-
Curvularia lunata
More the enzyme is a member of the short-chain dehydrogenase/reductase, SDR, superfamily Curvularia lunata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Curvularia lunata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
binding of the coenzyme NADPH to the enzyme causes local structural changes that do not significantly affect the thermal stability of this protein Curvularia lunata
60
-
enzyme oligomers are resistant to temperatures up to 60°C Curvularia lunata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Curvularia lunata

pH Range

pH Minimum pH Maximum Comment Organism
7 9
-
Curvularia lunata

pH Stability

pH Stability pH Stability Maximum Comment Organism
2
-
25°C, 150–300 mM NaCl, formation of soluble aggregates enriched in alpha-helical and beta-sheet structures, the soluble aggregates start to precipitate at higher temperatures and NaCl concentrations Curvularia lunata
7 9 the native dimeric enzyme is thermally stable up to 40°C in this pH range Curvularia lunata

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Curvularia lunata
NADPH binding of the coenzyme NADPH to the enzyme causes local structural changes that do not significantly affect the thermal stability of this protein Curvularia lunata