Application | Comment | Organism |
---|---|---|
medicine | enzyme is a potential drug target | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of isozymes AKR1C1-4 in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
isozyme AKR1C2 in ternary complex with NADP+ and ursodeoxycholate, X-ray diffraction structure determination and analysis at 3.0 A resolution, molecular replacement | Homo sapiens |
isozyme AKR1C9 in ternary complex with NADP+ and ursodeoxycholate, X-ray diffraction structure determination and analysis at 2.8 A resolution | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | cofactor binding kinetics | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-dihydroprogesterone + 2 NADPH + 2 H+ | Homo sapiens | isozyme AKR1C4 | pregnan-3alpha,20beta-diol + 2 NADP+ | - |
r | |
5alpha-dihydroprogesterone + 2 NADPH + 2 H+ | Rattus norvegicus | isozyme AKR1C9 | pregnan-3alpha,20beta-diol + 2 NADP+ | - |
r | |
additional information | Rattus norvegicus | enzyme regulates the occupancy and trans-activation of steroid hormone receptors by interconverting potent hormones with their cognate inactive metabolites | ? | - |
? | |
additional information | Homo sapiens | physiological roles of the isozymes AKR1C1-4, overview, enzyme regulates the occupancy and trans-activation of steroid hormone receptors by interconverting potent hormones with their cognate inactive metabolites | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
4 isozymes AKR1C1-4 | - |
Rattus norvegicus | - |
isozyme AKR1C9 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | steroid binding pocket structure of isozyme AKR1C2 | Homo sapiens | |
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | steroid binding pocket structure of isozyme AKR1C9 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | isozyme AKR1C4 | Homo sapiens | - |
prostate | isozyme AKR1C4 | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-dihydroprogesterone + 2 NADPH + 2 H+ | isozyme AKR1C4 | Homo sapiens | pregnan-3alpha,20beta-diol + 2 NADP+ | - |
r | |
5alpha-dihydroprogesterone + 2 NADPH + 2 H+ | isozyme AKR1C9 | Rattus norvegicus | pregnan-3alpha,20beta-diol + 2 NADP+ | - |
r | |
5alpha-dihydroprogesterone + 2 NADPH + 2 H+ | preferred substrate for isozyme AKR1C4, while isozymes AKR1C1-3 show only low activity | Homo sapiens | pregnan-3alpha,20beta-diol + 2 NADP+ | - |
r | |
additional information | enzyme regulates the occupancy and trans-activation of steroid hormone receptors by interconverting potent hormones with their cognate inactive metabolites | Rattus norvegicus | ? | - |
? | |
additional information | physiological roles of the isozymes AKR1C1-4, overview, enzyme regulates the occupancy and trans-activation of steroid hormone receptors by interconverting potent hormones with their cognate inactive metabolites | Homo sapiens | ? | - |
? | |
additional information | isozyme is positional- and stereospecific for the 3alpha-hydroxy steroid dehydrogenase reaction | Rattus norvegicus | ? | - |
? | |
additional information | isozymes display 3alpha-, 17beta-, and 20alpha-hydroxysteroid dehydrogenase activities to varying degrees, isozyme AKR1C4 shows the highest 3alpha-hydroxysteroid dehydrogenase activity of all isozymes, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | isozyme AKR1C2 shows an (alphabeta)8-barrel structure | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
3alpha-hydroxysteroid dehydrogenase | - |
Homo sapiens |
3alpha-hydroxysteroid dehydrogenase | - |
Rattus norvegicus |
AKR1C1-4 | - |
Homo sapiens |
AKR1C9 | - |
Rattus norvegicus |
HSD | - |
Homo sapiens |
HSD | - |
Rattus norvegicus |
More | enzyme belongs to the aldo-keto reductase superfamily | Homo sapiens |
More | enzyme belongs to the aldo-keto reductase superfamily | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
5alpha-dihydrotestosterone | isozyme AKR1C2 | Homo sapiens | |
1 | - |
5alpha-dihydrotestosterone | isozyme AKR1C9 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | binding and release mechanism | Homo sapiens | |
NADP+ | binding and release mechanism | Rattus norvegicus | |
NADPH | binding and release mechanism | Homo sapiens | |
NADPH | binding and release mechanism | Rattus norvegicus |