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Literature summary for 1.1.1.50 extracted from

  • Morikawa, T.; Tamaoki, B.
    Purification and characterization of 3alpha-hydroxysteroid dehydrogenase from chicken hepatic cytosol (1990), J. Steroid Biochem. Mol. Biol., 37, 569-574.
    View publication on PubMed

General Stability

General Stability Organism
not stable at 4°C Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
5beta-androstan-3,17-dione
-
Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Gallus gallus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00024
-
purified enzyme, substrate 5beta-androstan-3,17-dione Gallus gallus

Storage Stability

Storage Stability Organism
-20°C to -80°C, plus glycerol and 2-mercaptoethanol Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5beta-androstan-3,17-dione + NAD(P)H
-
Gallus gallus 5beta-androstan-17one-3-ol + NAD(P)+
-
?

Subunits

Subunits Comment Organism
? ? * 66000, SDS-PAGE Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Gallus gallus

Cofactor

Cofactor Comment Organism Structure
NADPH preference for Gallus gallus