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Literature summary for 1.1.1.49 extracted from
- Engineering a thermostable highly active glucose 6-phosphate dehydrogenase and its application to hydrogen production in vitro (2018), Appl. Microbiol. Biotechnol., 102, 3203-3215 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A117S/F277I/Q324H/M381I/V443I/S470I | t1/2 for the mutant enzyme at 60°C is 95fold higher than the t1/2 value for the wild-type enzyme | Zymomonas mobilis subsp. mobilis |
| A117S/Q324H/M381I/V443I/S470I | t1/2 for the mutant enzyme at 60°C is 75fold higher than the t1/2 value for the wild-type enzyme | Zymomonas mobilis subsp. mobilis |
| A117S/Q324H/V443I/S470I | t1/2 for the mutant enzyme at 60°C is 13.5fold higher than the t1/2 value for the wild-type enzyme | Zymomonas mobilis subsp. mobilis |
| L99I/A117S/G225S/F277I/Q324H/M381I/V443I/S470I/A476V | t1/2 for the mutant enzyme at 60°C is 124 fold higher than the t1/2 value for the wild-type enzyme. 3.4 °C increase in melting temperature (Tm), and a 5 °C increase in optimal temperature (Topt), without compromising the specific activity. The thermostable mutant is conducted to generate hydrogen from maltodextrin via in vitro synthetic biosystems, gaining a more than 8fold improvement of productivity rate with 76% of theoretical yield at 60°C | Zymomonas mobilis subsp. mobilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0078 | - |
D-glucose 6-phosphate | wild-type enzyme, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis |  |
| 0.0143 | - |
NADP+ | wild-type enzyme, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
| 0.0159 | - |
NADP+ | mutant enzyme L99I/A117S/G225S/F277I/Q324H/M381I/V443I/S470I/A476V, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
| 0.0199 | - |
NADP+ | mutant enzyme A117S/F277I/Q324H/M381I/V443I/S470I, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
| 0.0225 | - |
NADP+ | mutant enzyme A117S/Q324H/M381I/V443I/S470I, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
| 0.024 | - |
NADP+ | mutant enzyme A117S/Q324H/V443I/S470I, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
| 0.073 | - |
D-glucose 6-phosphate | mutant enzyme A117S/F277I/Q324H/M381I/V443I/S470I, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
| 0.081 | - |
D-glucose 6-phosphate | mutant enzyme L99I/A117S/G225S/F277I/Q324H/M381I/V443I/S470I/A476V, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
| 0.099 | - |
D-glucose 6-phosphate | mutant enzyme A117S/Q324H/M381I/V443I/S470I, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
| 0.151 | - |
D-glucose 6-phosphate | mutant enzyme A117S/Q324H/V443I/S470I, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NADP+ | Zymomonas mobilis subsp. mobilis | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
| D-glucose 6-phosphate + NADP+ | Zymomonas mobilis subsp. mobilis NRRL B-12526 | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zymomonas mobilis subsp. mobilis | - |
- |
- |
| Zymomonas mobilis subsp. mobilis NRRL B-12526 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 269.7 | - |
mutant enzyme A117S/F277I/Q324H/M381I/V443I/S470I, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis |
| 298.6 | - |
mutant enzyme L99I/A117S/G225S/F277I/Q324H/M381I/V443I/S470I/A476V, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis |
| 314.2 | - |
mutant enzyme A117S/Q324H/M381I/V443I/S470I, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis |
| 316.2 | - |
wild-type enzyme, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis |
| 326.9 | - |
mutant enzyme A117S/Q324H/V443I/S470I, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis |
| 741 | - |
mutant enzyme A117S/F277I/Q324H/M381I/V443I/S470I, pH 7.5, 60°C | Zymomonas mobilis subsp. mobilis |
| 772 | - |
mutant enzyme A117S/Q324H/V443I/S470I, pH 7.5, 60°C | Zymomonas mobilis subsp. mobilis |
| 847 | - |
mutant enzyme L99I/A117S/G225S/F277I/Q324H/M381I/V443I/S470I/A476V, pH 7.5, 60°C | Zymomonas mobilis subsp. mobilis |
| 850 | - |
mutant enzyme A117S/Q324H/M381I/V443I/S470I, pH 7.5, 60°C | Zymomonas mobilis subsp. mobilis |
| 852 | - |
wild-type enzyme, pH 7.5, 60°C | Zymomonas mobilis subsp. mobilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NADP+ | - |
Zymomonas mobilis subsp. mobilis | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
| D-glucose 6-phosphate + NADP+ | - |
Zymomonas mobilis subsp. mobilis NRRL B-12526 | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| G6PDH | - |
Zymomonas mobilis subsp. mobilis |
| glucose 6-phosphate dehydrogenase | - |
Zymomonas mobilis subsp. mobilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
t1/2 wild-type enzyme: 0.125 h. t1/2, mutant enzyme A117S/Q324H/V443I/S470I: 1.69 h. t1/2, mutant enzyme A117S/Q324H/M381I/V443I/S470I: 9.35 h. t1/2, mutant enzyme A117S/F277I/Q324H/M381I/V443I/S470I: 11.82 h. t1/2, mutant enzyme L99I/A117S/G225S/F277I/Q324H/M381I/V443I/S470I/A476V: 15.52 h | Zymomonas mobilis subsp. mobilis |
| 67.3 | - |
Tm-value, wild-type enzyme | Zymomonas mobilis subsp. mobilis |
| 68.8 | - |
Tm-value, mutant enzyme A117S/Q324H/V443I/S470I | Zymomonas mobilis subsp. mobilis |
| 69.6 | - |
Tm-value, mutant enzyme A117S/F277I/Q324H/M381I/V443I/S470I | Zymomonas mobilis subsp. mobilis |
| 69.7 | - |
Tm-value, mutant enzyme A117S/Q324H/M381I/V443I/S470I | Zymomonas mobilis subsp. mobilis |
| 70.7 | - |
Tm-value, mutant enzyme L99I/A117S/G225S/F277I/Q324H/M381I/V443I/S470I/A476V | Zymomonas mobilis subsp. mobilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 261 | - |
NADP+ | mutant enzyme A117S/F277I/Q324H/M381I/V443I/S470I, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
| 261 | - |
D-glucose 6-phosphate | mutant enzyme A117S/F277I/Q324H/M381I/V443I/S470I, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
| 288 | - |
NADP+ | mutant enzyme L99I/A117S/G225S/F277I/Q324H/M381I/V443I/S470I/A476V, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
| 288 | - |
D-glucose 6-phosphate | mutant enzyme L99I/A117S/G225S/F277I/Q324H/M381I/V443I/S470I/A476V, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
| 305 | - |
NADP+ | wild-type enzyme, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
| 305 | - |
D-glucose 6-phosphate | wild-type enzyme, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
| 306 | - |
NADP+ | mutant enzyme A117S/Q324H/M381I/V443I/S470I, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
| 306 | - |
D-glucose 6-phosphate | mutant enzyme A117S/Q324H/M381I/V443I/S470I, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
| 329 | - |
NADP+ | mutant enzyme A117S/Q324H/V443I/S470I, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
| 329 | - |
D-glucose 6-phosphate | mutant enzyme A117S/Q324H/V443I/S470I, pH 7.5, 30°C | Zymomonas mobilis subsp. mobilis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is one of the most important dehydrogenases responsible for generating reduced NADPH for anabolism and it is also the rate-limiting enzyme in the Entner-Doudoroff pathway | Zymomonas mobilis subsp. mobilis |
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