Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.49 extracted from

  • Ortiz, C.; Moraca, F.; Medeiros, A.; Botta, M.; Hamilton, N.; Comini, M.; Schmidt, T.
    Binding mode and selectivity of steroids towards glucose-6-phosphate dehydrogenase from the pathogen Trypanosoma cruzi (2016), Molecules, 21, 368.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.49

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Trypanosoma cruzi

Protein Variants

Protein Variants Comment Organism
K83A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Trypanosoma cruzi
K84A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Trypanosoma cruzi
L80G the mutant shows decreased catalytic efficiency compared to the wild type enzyme Trypanosoma cruzi
R408A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
(3alpha)-3,21-dihydroxypregnan-20-one
-
 Trypanosoma cruzi
16-bromoepiandrosterone no activity at 0.03 mM Trypanosoma cruzi
epiandrosterone uncompetitive inhibitor, 12% residual activity at 0.03 mM Trypanosoma cruzi
N-ethyl-N'-[(3alpha)-17-oxoandrostan-3-yl]urea
-
 Trypanosoma cruzi
N-[(3alpha)-17-oxoandrostan-3-yl]sulfuric diamide
-
 Trypanosoma cruzi
N-[(3alpha)-21-hydroxy-20-oxopregnan-3-yl]sulfuric diamide
-
 Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 NADP+ wild type enzyme, at pH 7.5 and 25°C Trypanosoma cruzi
0.017
-
 NADP+ mutant enzyme K84A, at pH 7.5 and 25°C Trypanosoma cruzi
0.017
-
 NADP+ mutant enzyme R408A, at pH 7.5 and 25°C Trypanosoma cruzi
0.021
-
 NADP+ mutant enzyme K83A, at pH 7.5 and 25°C Trypanosoma cruzi
0.074
-
 D-glucose 6-phosphate mutant enzyme L80G, at pH 7.5 and 25°C Trypanosoma cruzi
0.075
-
 NADP+ mutant enzyme L80G, at pH 7.5 and 25°C Trypanosoma cruzi
0.077
-
 D-glucose 6-phosphate wild type enzyme, at pH 7.5 and 25°C Trypanosoma cruzi
0.168
-
 D-glucose 6-phosphate mutant enzyme R408A, at pH 7.5 and 25°C Trypanosoma cruzi
0.537
-
 D-glucose 6-phosphate mutant enzyme K83A, at pH 7.5 and 25°C Trypanosoma cruzi
0.618
-
 D-glucose 6-phosphate mutant enzyme K84A, at pH 7.5 and 25°C Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + NADP+ Trypanosoma cruzi
-
 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q1WBU6
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 200 gel filtration Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + NADP+
-
 Trypanosoma cruzi 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
G6PDH
-
 Trypanosoma cruzi
glucose-6-phosphate dehydrogenase
-
 Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 D-glucose 6-phosphate mutant enzyme L80G, at pH 7.5 and 25°C Trypanosoma cruzi
2.7
-
 NADP+ mutant enzyme L80G, at pH 7.5 and 25°C Trypanosoma cruzi
2.8
-
 D-glucose 6-phosphate mutant enzyme K83A, at pH 7.5 and 25°C Trypanosoma cruzi
3
-
 NADP+ mutant enzyme K83A, at pH 7.5 and 25°C Trypanosoma cruzi
15
-
 D-glucose 6-phosphate mutant enzyme R408A, at pH 7.5 and 25°C Trypanosoma cruzi
19
-
 NADP+ mutant enzyme R408A, at pH 7.5 and 25°C Trypanosoma cruzi
24.6
-
 D-glucose 6-phosphate mutant enzyme K84A, at pH 7.5 and 25°C Trypanosoma cruzi
52
-
 NADP+ wild type enzyme, at pH 7.5 and 25°C Trypanosoma cruzi
58
-
 NADP+ mutant enzyme K84A, at pH 7.5 and 25°C Trypanosoma cruzi
62
-
 D-glucose 6-phosphate wild type enzyme, at pH 7.5 and 25°C Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Trypanosoma cruzi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0025
-
 epiandrosterone wild type enzyme, at pH 7.5 and 25°C Trypanosoma cruzi
0.003
-
 epiandrosterone mutant enzyme R408A, at pH 7.5 and 25°C Trypanosoma cruzi
0.008
-
 epiandrosterone mutant enzyme K84A, at pH 7.5 and 25°C Trypanosoma cruzi
0.011
-
 epiandrosterone mutant enzyme K83A, at pH 7.5 and 25°C Trypanosoma cruzi
0.031
-
 epiandrosterone mutant enzyme L80G, at pH 7.5 and 25°C Trypanosoma cruzi

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000015
-
 wild type enzyme, at pH 7.5 and 25°C Trypanosoma cruzi 16-bromoepiandrosterone
0.0015
-
 wild type enzyme, at pH 7.5 and 25° Trypanosoma cruzi N-ethyl-N'-[(3alpha)-17-oxoandrostan-3-yl]urea
0.0022
-
 wild type enzyme, at pH 7.5 and 25° Trypanosoma cruzi N-[(3alpha)-17-oxoandrostan-3-yl]sulfuric diamide
0.003
-
 wild type enzyme, at pH 7.5 and 25°C Trypanosoma cruzi epiandrosterone

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5
-
 D-glucose 6-phosphate mutant enzyme K83A, at pH 7.5 and 25°C Trypanosoma cruzi
27
-
 D-glucose 6-phosphate mutant enzyme L80G, at pH 7.5 and 25°C Trypanosoma cruzi
36
-
 NADP+ mutant enzyme L80G, at pH 7.5 and 25°C Trypanosoma cruzi
40
-
 D-glucose 6-phosphate mutant enzyme K84A, at pH 7.5 and 25°C Trypanosoma cruzi
89
-
 D-glucose 6-phosphate mutant enzyme R408A, at pH 7.5 and 25°C Trypanosoma cruzi
140
-
 NADP+ mutant enzyme K83A, at pH 7.5 and 25°C Trypanosoma cruzi
800
-
 D-glucose 6-phosphate wild type enzyme, at pH 7.5 and 25°C Trypanosoma cruzi
1100
-
 NADP+ mutant enzyme R408A, at pH 7.5 and 25°C Trypanosoma cruzi
3200
-
 NADP+ wild type enzyme, at pH 7.5 and 25°C Trypanosoma cruzi
3400
-
 NADP+ mutant enzyme K84A, at pH 7.5 and 25°C Trypanosoma cruzi