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Literature summary for 1.1.1.49 extracted from

  • Banerjee, S.; Fraenkel, D.G.
    Glucose-6-phosphate dehydrogenase from Escherichia coli and from a "high-level" mutant (1972), J. Bacteriol., 110, 155-160.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.49

Inhibitors

Inhibitors Comment Organism Structure
ATP 10 mM, 50-75% inhibition, assay without MgCl2 Escherichia coli
Cd2+ 1 mM, 83% inhibition Escherichia coli
Co2+ 1 mM, 56% inhibition Escherichia coli
Cu2+ 1 mM, complete inhibition Escherichia coli
Hg2+ 1 mM, complete inhibition Escherichia coli
Mn2+ 1 mM, 56% inhibition Escherichia coli
NADPH
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 NADP+ 25°C, pH 8.0, high-level glucose-6-phosphate dehydrogenase strain DF82 Escherichia coli
0.016
-
 NADP+ 25°C, pH 8.0, wild-type strain K-10 Escherichia coli
0.1
-
 D-glucose 6-phosphate 25°C, pH 8.0, wild-type strain K-10 Escherichia coli
0.145
-
 D-glucose 6-phosphate 25°C, pH 8.0, high-level glucose-6-phosphate dehydrogenase strain DF82 Escherichia coli
1.67
-
 NAD+ 25°C, pH 8.0 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 1.4fold activation Escherichia coli
Mg2+ 1 mM, 1.6fold activation Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 x * 52000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + NADP+ Escherichia coli
-
 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?
D-glucose 6-phosphate + NADP+ Escherichia coli K-10
-
 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?
D-glucose 6-phosphate + NADP+ Escherichia coli DF82
-
 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli
-
 high-level glucose-6-phosphate dehydrogenase strain
-
Escherichia coli DF82
-
 high-level glucose-6-phosphate dehydrogenase strain
-
Escherichia coli K-10
-
-
-

Purification (Commentary)

Purification (Comment) Organism
approximately 100-fold purification Escherichia coli
approximately 450fold purification Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
100
-
 25°C, pH 8.0, high-level glucose-6-phosphate dehydrogenase strain DF82 Escherichia coli
104
-
 25°C, pH 8.0, wild-type strain K-10 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + NAD+
-
 Escherichia coli 6-phospho-D-glucono-1,5-lactone + NADH + H+
-
 ?
D-glucose 6-phosphate + NAD+
-
 Escherichia coli K-10 6-phospho-D-glucono-1,5-lactone + NADH + H+
-
 ?
D-glucose 6-phosphate + NAD+
-
 Escherichia coli DF82 6-phospho-D-glucono-1,5-lactone + NADH + H+
-
 ?
D-glucose 6-phosphate + NADP+
-
 Escherichia coli 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?
D-glucose 6-phosphate + NADP+
-
 Escherichia coli K-10 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?
D-glucose 6-phosphate + NADP+
-
 Escherichia coli DF82 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?
additional information the following substances, 1 mM, are not substrates when substituted for D-glucose 6-phosphate: D-glucose 6-phosphate, D-fructose 1-phosphate, D-gluconate 6-phosphate, D-galactose 6-phosphate, D-ribose 5-phosphate, and D-glucose Escherichia coli ?
-
 ?
additional information the following substances, 1 mM, are not substrates when substituted for D-glucose 6-phosphate: D-glucose 6-phosphate, D-fructose 1-phosphate, D-gluconate 6-phosphate, D-galactose 6-phosphate, D-ribose 5-phosphate, and D-glucose Escherichia coli K-10 ?
-
 ?
additional information the following substances, 1 mM, are not substrates when substituted for D-glucose 6-phosphate: D-glucose 6-phosphate, D-fructose 1-phosphate, D-gluconate 6-phosphate, D-galactose 6-phosphate, D-ribose 5-phosphate, and D-glucose Escherichia coli DF82 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 52000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6.2 9.8 pH 6.2: about 50% of maximal activity, pH 9.8: about 50% of maximal activity Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD+ can replace NADP+, with 10 mM NAD+ giving 25% of the rate in the usual assay with NADP+ Escherichia coli
NADP+
-
 Escherichia coli