Cloned (Comment) | Organism |
---|---|
gene gpd, expression in Escherichia coli strain BL21(DE3) as His-tagged enzyme | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
D-glucose 6-phosphate | pH 7.8, 85°C, recombinant enzyme | Thermotoga maritima | |
0.08 | - |
NADP+ | pH 7.8, 85°C, recombinant enzyme | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57500 | - |
about, recombinant enzyme, native PAGE | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Thermotoga maritima |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
76 | - |
purified recombinant enzyme, development of a coupled continuous thermostable assay method, determination at 85°C | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | - |
Thermotoga maritima | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 x 57500, recombinant enzyme, SDS-PAGE | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
glucose-6-phosphate dehydrogenase | - |
Thermotoga maritima |
GPD | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 90 | over 60% of maximal activity at 70°C and 90°C, recombinant enzyme | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | recombinant enzyme | Thermotoga maritima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Thermotoga maritima |