Application | Comment | Organism |
---|---|---|
biotechnology | design and synthesis by protein molecular modelling and ligand docking of several chimeric mimodye-ligands comprising a NAD-pseudomimetic moiety of anthraquinone diaminobenzosulfonic acid and a galactosyl-mimetic moiety of 2-amino-2-deoxygalactose or shikimic acid for usage as tailored ligands in selective affinity chromatography during enzyme purification/production, immobilization on a gel resin, overview | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
anthraquinone-diamino-benzosulfonyl-dichlorotriazine | i.e. Vilmafix Blue A-R, and other derivatives of longer side chain length, inactivation, overview | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by affinity chromatography on chimeric mimodye-ligand gel resins, method evaluation | Pseudomonas fluorescens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | recombinant, purified | Pseudomonas fluorescens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactopyranose + NAD(P)+ | - |
Pseudomonas fluorescens | D-galactono-1,5-lactone + NAD(P)H | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure modelling and computational analysis | Pseudomonas fluorescens |
Synonyms | Comment | Organism |
---|---|---|
beta-galactose dehydrogenase | - |
Pseudomonas fluorescens |
D-galactose: NAD+ 1-oxidoreductase | - |
Pseudomonas fluorescens |
GADH | - |
Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | preferred cofactor | Pseudomonas fluorescens | |
NADP+ | - |
Pseudomonas fluorescens |