Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Tetranitromethane | the rapid inactivation can be prevented by the presence of NAD, AMP, or ATP | Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
4 * 30000, gel filtration | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | P40288 | - |
- |
Priestia megaterium IWG3 | P40288 | - |
- |
Purification (Comment) | Organism |
---|---|
Sephadex G-100 gel filtration | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + NAD(P)+ | - |
Priestia megaterium | D-glucono-1,5-lactone + NAD(P)H | - |
? | |
D-glucose + NAD(P)+ | - |
Priestia megaterium IWG3 | D-glucono-1,5-lactone + NAD(P)H | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 30000, gel filtration | Priestia megaterium |
Synonyms | Comment | Organism |
---|---|---|
glucose dehydrogenase | - |
Priestia megaterium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
after dissociation of the tetrameric enzyme into its inactive monomers at pH 9 two tyrosine residues (Tyr-254 and Tyr-160) become susceptible to nitration which are unable to reassociate to the tetramer | Priestia megaterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Priestia megaterium | |
NADP+ | - |
Priestia megaterium |