Literature summary for 1.1.1.47 extracted from

Pire, C.; Marhuenda-Egea, F.C.; Esclapez, J.; Alcaraz, L.; Ferrer, J.; Bonete, M.J.
Stability and enzymatic studies of glucose dehydrogenase from the archaeon Haloferax mediterranei in reverse micelles (2004), Biocatal. Biotransform., 22, 17-23.

No PubMed abstract available

Search for more literature for 1.1.1.47

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	hexadecyltrimethylammonium bromide, i.e. CTAB, does not influence the enzyme activity but increases the enzyme stability	Haloferax mediterranei

Application

Application	Comment	Organism
synthesis	enzyme can be used for gluconic acid production in low water systems	Haloferax mediterranei

General Stability

General Stability	Organism
enzyme shows good stability at low water content and at low salt concentration in reverse micelles	Haloferax mediterranei

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	hexadecyltrimethylammonium bromide, i.e. CTAB, does not influence the enzyme activity but increases the enzyme stability	Haloferax mediterranei

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Haloferax mediterranei	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
MgCl2	25 mM for activity assay	Haloferax mediterranei
additional information	extreme halophilic enzyme	Haloferax mediterranei
NaCl	2 M for activity assay	Haloferax mediterranei

Organic Solvent Stability

Organic Solvent	Comment	Organism
cyclohexane	enzyme is solubilized in reverse micelles of hexadecyltrimethylammonium bromide in cyclohexane with n-butanol as cosurfactant and retaines activity and catalytic properties in the organic medium	Haloferax mediterranei
n-Butanol	enzyme is solubilized in reverse micelles of hexadecyltrimethylammonium bromide in cyclohexane with n-butanol as cosurfactant and retaines activity and catalytic properties in the organic medium	Haloferax mediterranei

Organism

Organism	UniProt	Comment	Textmining
Haloferax mediterranei	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme is solubilized in reverse micelles of hexadecyltrimethylammonium bromide, i.e. CTAB, in cyclohexane with n-butanol as cosurfactant	Haloferax mediterranei

Reaction

Reaction	Comment	Organism	Reaction ID
D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H + H+	sequential type ordered bibi mechnanism	Haloferax mediterranei	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-D-glucose + NAD+	-	Haloferax mediterranei	D-glucono-1,5-lactone + NADH + H+	-	?

Synonyms

Synonyms	Comment	Organism
GDH	-	Haloferax mediterranei
glucose dehydrogenase	-	Haloferax mediterranei

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	assay at	Haloferax mediterranei

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.8	-	assay at	Haloferax mediterranei

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Haloferax mediterranei

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Release 2023.1 (January 2023)