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Literature summary for 1.1.1.47 extracted from
- Location and properties of glucose dehydrogenase in sporulating cells and spores of Bacillus subtilis (1977), J. Bacteriol., 132, 282-293.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
NAD+ | pH 7.8, 23°C | Bacillus subtilis |  |
| 0.26 | - |
NADP+ | pH 7.8, 30°C | Bacillus subtilis | |
| 42.9 | - |
beta-D-glucose | pH 7.8, 30°C, coenzyme NADP+ | Bacillus subtilis | |
| 47.8 | - |
2-deoxy-beta-D-glucose | pH 7.8, 23°C | Bacillus subtilis | |
| 55.9 | - |
beta-D-glucose | pH 7.8, 30°C | Bacillus subtilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| forespore | - |
Bacillus subtilis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 115000 | - |
gel filtration | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration, DEAE-cellulose, hydroxylapatite, omega-aminohexyl-agarose | Bacillus subtilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spore | - |
Bacillus subtilis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26 | - |
- |
Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20% glycerol, 3 months | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-beta-D-xylose + NAD+ | - |
Bacillus subtilis | 2-deoxy-D-xylono-1,5-lactone + NADH + H+ | - |
r | |
| 2-deoxy-D-glucose + NAD+ | 108% of the activity with D-glucose | Bacillus subtilis | 2-deoxy-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
| beta-D-glucose + NAD+ | - |
Bacillus subtilis | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
| D-galactose + NADP+ | 5% of the activity with D-glucose | Bacillus subtilis | D-galactono-1,5-lactone + NADPH | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
Tris-chloride buffer | Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | pH 7.0: 70% of maximal activity with glucose and NAD+ and about 55% of the activity with glucose and NADP+, pH 8.5: about 55% of maximal activity with glucose and NAD+ and about 75% of the activity with glucose and NADP+ | Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
stable at | Bacillus subtilis |
| 8 | - |
rapid inactivation | Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Bacillus subtilis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus subtilis | isoelectric focusing | - |
4.9 |
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