Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
AMP | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
ATP | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
dipicolinic acid | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
NAD+ | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
NADH | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
NADP+ | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
NADPH | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) |
General Stability | Organism |
---|---|
crude enzyme loses 90% activity after 3 days at 5°C | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.061 | - |
NADP+ | - |
Bacillus sp. (in: Bacteria) | |
0.082 | - |
NAD+ | - |
Bacillus sp. (in: Bacteria) | |
4.2 | - |
beta-D-glucose | - |
Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
KCl | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
Na2SO4 | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
NaCl | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
inactive enzyme form, gel filtration | Bacillus sp. (in: Bacteria) |
80000 | - |
active enzyme form, gel filtration | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
D-ribose producing mutant | - |
Bacillus sp. (in: Bacteria) BG 1722 | - |
D-ribose producing mutant | - |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose, partially purified enzyme, 10 mM NAD(P)+ protects from inactivation on column | Bacillus sp. (in: Bacteria) |
Storage Stability | Organism |
---|---|
4°C, 89% loss of activity after 4 days without stabilzing agent, complete stabilization by 10 mM NAD+ or 10 mM NADP+, and to a lesser extent by 800 mM NaCl. Partial stabilization by 800 MM (NH4)2SO4, 800 mM Na2SO4 or 800 mM dipicolinic acid | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + NAD+ | 113% of the activity with D-glucose | Bacillus sp. (in: Bacteria) | 2-deoxy-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
2-deoxy-D-glucose + NAD+ | 113% of the activity with D-glucose | Bacillus sp. (in: Bacteria) BG 1722 | 2-deoxy-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
2-deoxy-D-glucose + NADP+ | 103% of the activity with D-glucose and NAD+ | Bacillus sp. (in: Bacteria) | 2-deoxy-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
2-deoxy-D-glucose + NADP+ | 103% of the activity with D-glucose and NAD+ | Bacillus sp. (in: Bacteria) BG 1722 | 2-deoxy-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
beta-D-glucose + NADP+ | 93% of the activity with NAD+ | Bacillus sp. (in: Bacteria) | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
beta-D-glucose + NADP+ | 93% of the activity with NAD+ | Bacillus sp. (in: Bacteria) BG 1722 | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
maltose + NAD+ | 6% of the activity with D-glucose | Bacillus sp. (in: Bacteria) | ? + NADH | - |
? | |
maltose + NAD+ | 6% of the activity with D-glucose | Bacillus sp. (in: Bacteria) BG 1722 | ? + NADH | - |
? | |
maltose + NADP+ | 5% of the activity D-glucose and NAD+ | Bacillus sp. (in: Bacteria) | ? + NADPH | - |
? | |
maltose + NADP+ | 5% of the activity D-glucose and NAD+ | Bacillus sp. (in: Bacteria) BG 1722 | ? + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | - |
Bacillus sp. (in: Bacteria) |
tetramer | 2 * 45000, gel filtration of inactive monomeric enzyme | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Bacillus sp. (in: Bacteria) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus sp. (in: Bacteria) | |
NADP+ | - |
Bacillus sp. (in: Bacteria) |