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Literature summary for 1.1.1.47 extracted from
- Crystallization and characterization of NADP-dependent D-glucose dehydrogenase from Gluconobacter suboxydans (1980), Agric. Biol. Chem., 44, 301-308.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| precipitate collected by centrifugation and dissolved in a minimal volume of 0.1 M potassium phosphate buffer, pH 7.5, 1 mM 2-mercaptoethanol | Gluconobacter oxydans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Gluconobacter oxydans |  |
| Hg2+ | - |
Gluconobacter oxydans | |
| Ni2+ | - |
Gluconobacter oxydans | |
| PCMB | - |
Gluconobacter oxydans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
NADP+ | pH 8.0, 25°C | Gluconobacter oxydans | |
| 5 | - |
D-glucose | pH 8.0, 25°C | Gluconobacter oxydans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Gluconobacter oxydans | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
4 * 40000, SDS-PAGE | Gluconobacter oxydans |
| 153000 | - |
gel filtration | Gluconobacter oxydans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gluconobacter oxydans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sephadex, Blue-Dextran Sepharose, DEAE-Sephadex, crystallization | Gluconobacter oxydans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 140 | - |
- |
Gluconobacter oxydans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, crystalline preparation as suspension in ammonium sulfate, stable for 6 months | Gluconobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-glucose + NADP+ | in the reverse reaction only 4% of the reaction occurs at neutral pH | Gluconobacter oxydans | D-glucono-1,5-lactone + NADPH + H+ | - |
r | |
| beta-D-glucose + NADP+ | the enzyme is specific for NADP+ and completely inactive with NAD+ | Gluconobacter oxydans | D-glucono-1,5-lactone + NADPH + H+ | - |
r | |
| D-glucose + NADP+ | - |
Gluconobacter oxydans | D-glucono-1,5-lactone + NADPH + H+ | - |
r | |
| D-mannose + NADP+ | 88% of the activity with D-glucose | Gluconobacter oxydans | D-mannono-1,5-lactone + NADPH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 40000, SDS-PAGE | Gluconobacter oxydans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Gluconobacter oxydans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Gluconobacter oxydans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | the enzyme is specific for NADP+ and completely inactive with NAD+ | Gluconobacter oxydans | |
| NADP+ | the enzyme is specific for NADP+ and completely inactive with NAD+ | Gluconobacter oxydans | |
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