Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Oryctolagus cuniculus |
Protein Variants | Comment | Organism |
---|---|---|
D36R | mutation leads to a switch in the favor of NADP(H) specificity, suggesting an important role of Asp36 in the coenzyme specificity | Oryctolagus cuniculus |
E157Q | mutation produces no large kinetic alterations | Oryctolagus cuniculus |
H145Q | inactive enzyme form | Oryctolagus cuniculus |
N195D | inactive enzyme form | Oryctolagus cuniculus |
N196Q | inactive enzyme form | Oryctolagus cuniculus |
S124A | mutation decreases the catalytic efficiency 500 fold | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cibacron blue | competitive inhibition with respect to NAD+ | Oryctolagus cuniculus | |
malonate | reversible inhibition, IC50: 0.34 mM | Oryctolagus cuniculus | |
malonic acid monoethyl ester | weak inhibition, IC50: 5.5 mM | Oryctolagus cuniculus | |
additional information | no inhibition by 20 mM D-glucose, 2 mM 2-keto-L-gulonate, 1 mM D-glucorunate or 6 mM ADP, ATP and AMP; no significant inhibition (less than 15%) by 1 mM L-ascorbate, monocarboxylic acids, or dicarboxylic acids | Oryctolagus cuniculus | |
p-chloromercuriphenylsulfonate | 0.001 mM, complete inhibition | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | no activity with 5-40 mM sugars or hydroxyacids like 2-keto-L-guluonate, 3-deoxyglucosone, xylitol, L-threitol, ascorbic acid, malic acid, L-threonine, L-glycerate, L-tartarate and L-lactate, or 50 mM ethyl and methyl esters of 3-hydroxybutyrate | Oryctolagus cuniculus | |
0.0005 | - |
NADH | at pH 8.5, rabbit liver GDH | Oryctolagus cuniculus | |
0.0006 | - |
NADH | at pH 8.5, recombinant rabbit GDH | Oryctolagus cuniculus | |
0.01 | - |
NAD+ | at pH 8.5, recombinant rabbit GDH | Oryctolagus cuniculus | |
0.011 | - |
NAD+ | at pH 8.5, rabbit liver GDH | Oryctolagus cuniculus | |
0.18 | - |
L-gulonate | at pH 8.5, recombinant rabbit GDH | Oryctolagus cuniculus | |
0.21 | - |
L-gulonate | at pH 8.5, rabbit liver GDH | Oryctolagus cuniculus | |
0.49 | - |
L-gulonate | mutant enzyme E157Q, NAD+-linked activity | Oryctolagus cuniculus | |
0.67 | - |
NADP+ | at pH 8.5, recombinant rabbit GDH | Oryctolagus cuniculus | |
0.89 | - |
NADP+ | at pH 8.5, rabbit liver GDH | Oryctolagus cuniculus | |
1.2 | - |
L-threonate | at pH 8.5, recombinant rabbit GDH | Oryctolagus cuniculus | |
2.2 | - |
L-3-hydroxybutyrate | at pH 8.5, recombinant rabbit GDH | Oryctolagus cuniculus | |
2.3 | - |
L-3-hydroxybutyrate | at pH 8.5, rabbit liver GDH | Oryctolagus cuniculus | |
2.9 | - |
L-threonate | at pH 8.5, rabbit liver GDH | Oryctolagus cuniculus | |
4.4 | - |
L-gulonate | mutant enzyme S124A, NAD+-linked activity | Oryctolagus cuniculus | |
8.3 | - |
acetoacetate | at pH 8.5, rabbit liver GDH | Oryctolagus cuniculus | |
9.9 | - |
acetoacetate | at pH 8.5, recombinant rabbit GDH | Oryctolagus cuniculus | |
14 | - |
L-gulonate | mutant enzyme D36R, NAD+-linked activity | Oryctolagus cuniculus | |
18.5 | - |
L-gulonate | mutant enzyme D36R, NADP+-linked activity | Oryctolagus cuniculus | |
18.5 | - |
L-gulonate | mutant enzyme E157Q, NADP+-linked activity | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000 | Oryctolagus cuniculus |
36000 | - |
SDS-PAGE | Oryctolagus cuniculus |
70000 | - |
gel filtration | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
Japanese white rabbit | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, Sephadex G-100 column chromatography, Q-Sepharose column chromatography, Blue-Sepharose column chromatography, hydroxylapatite column chromatography | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lens | - |
Oryctolagus cuniculus | - |
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.011 | - |
in the supernatant | Oryctolagus cuniculus |
6.71 | - |
after purification | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
? + NADP+ | - |
Oryctolagus cuniculus | ? + NADPH | - |
? | |
acetoacetate + NADH | - |
Oryctolagus cuniculus | ? + NAD+ | - |
? | |
L-3-hydroxybutyrate + ? | - |
Oryctolagus cuniculus | ? | - |
? | |
L-gulonate + NAD+ | - |
Oryctolagus cuniculus | 3-dehydro-L-gulonate + NADH + H+ | - |
? | |
L-threonate + ? | - |
Oryctolagus cuniculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000 | Oryctolagus cuniculus |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Oryctolagus cuniculus | |
NADP+ | - |
Oryctolagus cuniculus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
7.3 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.34 | - |
reversible inhibition, IC50: 0.34 mM | Oryctolagus cuniculus | malonate | |
5.5 | - |
weak inhibition, IC50: 5.5 mM | Oryctolagus cuniculus | malonic acid monoethyl ester |