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Literature summary for 1.1.1.45 extracted from

  • Cannistraro, V.J.; Borack, L.I.; Chase, T.
    Subunit structure and kinetic properties of L-beta-hydroxyacid dehydrogenase of Drosophila (1979), Biochim. Biophys. Acta, 569, 1-5.
    View publication on PubMed

General Stability

General Stability Organism
3 M urea results in loss of activity of 83% in 7 min Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
acetoacetate competitive inhibitor Drosophila melanogaster
NADH competitive inhibitor Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
NAD+ cosubstrate L-gulonate, pH 8.2 Drosophila melanogaster
0.13
-
NAD+ cosubstrate L-gulonate, pH 8.2 Drosophila melanogaster
0.85
-
L-gulonate cofactor NAD+, pH 8.2 Drosophila melanogaster
14.9
-
L-3-hydroxybutanoate cofactor NAD+, pH 8.2 Drosophila melanogaster

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23500
-
alphabeta, 1 * 23500 + 1 * 40000, SDS-PAGE Drosophila melanogaster
40000
-
alphabeta, 1 * 23500 + 1 * 40000, SDS-PAGE Drosophila melanogaster
63500
-
SDS-PAGE Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-gulonic acid + NAD+ Drosophila melanogaster
-
3-dehydro-L-gulonic acid + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
-
Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-3-hydroxybutanoate + NADH + H+
-
Drosophila melanogaster acetoacetate + NAD+
-
r
L-3-hydroxybutanoate + NADH
-
Drosophila melanogaster acetoacetate + NAD+
-
?
L-gulonic acid + NAD+
-
Drosophila melanogaster 3-dehydro-L-gulonic acid + NADH + H+
-
?
L-gulonic acid + NAD+
-
Drosophila melanogaster 3-dehydro-L-gulonic acid + NADH + H+
-
r

Subunits

Subunits Comment Organism
dimer alphabeta, 1 * 23500 + 1 * 40000, SDS-PAGE Drosophila melanogaster

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.733
-
DL-3-hydroxybutanoate
-
Drosophila melanogaster
7.4
-
L-gulonate
-
Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Drosophila melanogaster
NADH
-
Drosophila melanogaster
NADP+ 10% activity of NAD+ Drosophila melanogaster