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Literature summary for 1.1.1.45 extracted from

  • Smiley, J.D.; Ashwell, G.
    Purification and properties of beta-L-hydroxy acid dehydrogenase (1961), J. Biol. Chem., 236, 357-364.
No PubMed abstract available
Search for more literature for 1.1.1.45

Activating Compound

Activating Compound Comment Organism Structure
cysteine in mM-concentration, no activation of the reverse reaction Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory iodoacetate, ethylendiamine tetraacetate, 2,2'-bipyridine, 8-hydroxyquiniline, O-phenanthroline, diethyldithiocarbamate, thiamine pyrophosphate Sus scrofa
p-chloromercuribenzoate in both directions Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic studies Sus scrofa
0.04
-
 NAD+ cosubstrate L-gulonate, pH 8.5 Sus scrofa
0.45
-
 beta-keto-L-gulonate cofactor NADH, pH 6.3 Sus scrofa
5.1
-
 L-gulonate cofactor NAD+, pH 8.5 Sus scrofa
5.3
-
 L-3-hydroxybutanoate
-
 Sus scrofa
5.3
-
 L-Idonate cofactor NAD+, pH 8.5 Sus scrofa
6.4
-
 acetoacetate cofactor NADH, pH 6.3 Sus scrofa
7.3
-
 2,3-diketo-L-gulonate cofactor NADH, pH 6.3 Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Sus scrofa 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no activation or inhibition Zn2+, Cd2+, Cu2+, Fe3+, Mn2+, Ni2+, Co3+, As3+ Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-gulonic acid + NAD+ Sus scrofa glucuronate-xylulose cycle 3-dehydro-L-gulonic acid + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
 hog
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Sus scrofa
-

Storage Stability

Storage Stability Organism
-18°C, 0.05 M K2HPO4, several weeks, little loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-keto-L-gulonate + NADH
-
 Sus scrofa 2,3-diketo-L-gulonate + NAD+
-
 ir
D-galactonic acid + NAD+
-
 Sus scrofa ?
-
 ?
D-gluconic acid + NAD+
-
 Sus scrofa ?
-
 ?
D-lyxonic acid + NAD+
-
 Sus scrofa ?
-
 ?
D-mannonic acid + NAD+
-
 Sus scrofa ?
-
 ?
D-talonic acid + NAD+
-
 Sus scrofa ?
-
 ?
D-xylonic acid + NAD+
-
 Sus scrofa ?
-
 ?
L-3-hydroxybutanoate + NADH
-
 Sus scrofa acetoacetate + NAD+
-
 r
L-altronic acid + NAD+
-
 Sus scrofa ?
-
 ?
L-erythronic acid + NAD+
-
 Sus scrofa ?
-
 ?
L-gulonic acid + NAD+ stereospecificity for L-configuration of the beta-carbon hydroxyl group Sus scrofa 3-dehydro-L-gulonic acid + NADH + H+
-
 r
L-gulonic acid + NAD+ glucuronate-xylulose cycle Sus scrofa 3-dehydro-L-gulonic acid + NADH + H+
-
 r
L-idonic acid + NAD+
-
 Sus scrofa ?
-
 ?
L-ribonic acid + NAD+
-
 Sus scrofa ?
-
 ?
L-threonic acid + NAD+
-
 Sus scrofa ?
-
 ?
additional information no substrates are: L-arabonic acid, D-glucuronate, D-mannuronate, l-iduronate, 6-phospho-D-gluconate, L-serine, L-threonine, beta-D-hydroxy acids, acetoacetyl-CoA, acetoacetyl-S-pantetheine Sus scrofa ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
 reduction of 3-dehydro-L-gulonic acid Sus scrofa
8.5
-
-
 Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
4 8 reduction of acetoacetate Sus scrofa
6.2 10 oxidation of L-3-hydroxybutanoate Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Sus scrofa
NADH
-
 Sus scrofa