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Literature summary for 1.1.1.44 extracted from

  • Sahin, S.
    Stability evaluation of 6-phosphogluconate dehydrogenase immobilized on amino-functionalized magnetic nanoparticles (2019), Prep. Biochem. Biotechnol., 49, 590-596 .
    View publication on PubMed
Search for more literature for 1.1.1.44

Application

Application Comment Organism
biotechnology immobilization of 6PDGH on ASMNPs can be an effective way for its biotechnological and biosensor applications Saccharomyces cerevisiae

General Stability

General Stability Organism
enzyme immobilized on 2-aminoethyl-3-aminopropyltriethoxysilane modified core-shell Fe3O4SiO2 magnetic nanoparticles maintains 62% of its initial activity after five cycles Saccharomyces cerevisiae
thermal, and storage stability of the enzyme (6PGDH) are improved with covalent immobilization of the enzyme (6PGDH) on 2-aminoethyl-3-aminopropyltriethoxysilane modified core-shell Fe3O4SiO2 magnetic nanoparticles Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-phospho-D-gluconate + NADP+ Saccharomyces cerevisiae
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Storage Stability

Storage Stability Organism
4°C, enzyme immobilized on 2-aminoethyl-3-aminopropyltriethoxysilane modified core-shell Fe3O4SiO2 magnetic nanoparticles maintains 35% of its initial activity after 22 days of storage Saccharomyces cerevisiae
4°C, the soluble enzyme completely loses its initial activity after 15 days Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NADP+
-
 Saccharomyces cerevisiae D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
6-phospho-D-gluconate + NADP+ while the soluble enzyme (6PGDH) converted 33% of NADP+ in reaction medium upon 480 s, the immobilized enzyme performs 56% conversion of NADP+ at same time Saccharomyces cerevisiae D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
6-phosphogluconate dehydrogenase
-
 Saccharomyces cerevisiae
6PGDH
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 both of the soluble and immobilized enzyme have the maximum thermal stability at 30°C Saccharomyces cerevisiae
40
-
 1 h, immobilized enzyme loses 84% of its initial activity, soluble enzyme loses 89% of its initial activity Saccharomyces cerevisiae
50
-
 1 h, immobilized enzyme loses 92% of its initial activity, soluble enzyme loses 97% of its initial activity Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Saccharomyces cerevisiae