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Literature summary for 1.1.1.44 extracted from

  • Tsai, C.S.; Chen, Q.
    Purification and kinetic characterization of 6-phosphogluconate dehydrogenase from Schizosaccharomyces pombe (1998), Biochem. Cell Biol., 76, 637-644.
    View publication on PubMed
Search for more literature for 1.1.1.44

Cloned(Commentary)

Cloned (Comment) Organism
cloning of the LT2 gnd gene Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Ovis aries

Inhibitors

Inhibitors Comment Organism Structure
D-ribulose 5-phosphate
-
 Cyberlindnera jadinii
D-ribulose 5-phosphate
-
 Schizosaccharomyces pombe
NADPH
-
 Dicentrarchus labrax
NADPH
-
 Neurospora crassa
NADPH competitive inhibition Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activated by divalent metal ions Schizosaccharomyces pombe
Mn2+ activated by divalent metal ions Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 4 * 38000, SDS-PAGE Schizosaccharomyces pombe
158000
-
 gel filtration Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-phospho-D-gluconate + NADP+ Salmonella enterica subsp. enterica serovar Typhimurium
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+ Cyberlindnera jadinii
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+ Pseudomonas sp.
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+ Leuconostoc mesenteroides
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+ Gluconobacter oxydans
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+ Neurospora crassa enzyme specific for the oxidative pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+ Ovis aries enzyme specific for the oxidative pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+ Dicentrarchus labrax enzyme specific for the oxidative pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+ Schizosaccharomyces pombe pivotal enzyme links the gluconate route and the oxidative phase of the pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Cyberlindnera jadinii
-
-
-
Dicentrarchus labrax
-
 bass
-
Gluconobacter oxydans
-
-
-
Leuconostoc mesenteroides
-
-
-
Neurospora crassa
-
-
-
Ovis aries
-
 lamb
-
Ovis aries
-
 sheep
-
Pseudomonas sp.
-
 P. fluorescens
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
 LT2
-
Schizosaccharomyces pombe
-
 fission yeast, NCYC 132S2-2
-

Purification (Commentary)

Purification (Comment) Organism
-
 Schizosaccharomyces pombe

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Ovis aries
-
liver
-
 Dicentrarchus labrax
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
39.4
-
-
 Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NADP+
-
 Salmonella enterica subsp. enterica serovar Typhimurium D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+
-
 Cyberlindnera jadinii D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+
-
 Neurospora crassa D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+
-
 Ovis aries D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+
-
 Pseudomonas sp. D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+
-
 Schizosaccharomyces pombe D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+
-
 Leuconostoc mesenteroides D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+
-
 Gluconobacter oxydans D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+
-
 Dicentrarchus labrax D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+ enzyme specific for the oxidative pentose phosphate pathway Neurospora crassa D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+ enzyme specific for the oxidative pentose phosphate pathway Ovis aries D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+ enzyme specific for the oxidative pentose phosphate pathway Dicentrarchus labrax D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
6-phospho-D-gluconate + NADP+ pivotal enzyme links the gluconate route and the oxidative phase of the pentose phosphate pathway Schizosaccharomyces pombe D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 38000, SDS-PAGE Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Cyberlindnera jadinii
7.5
-
-
 Schizosaccharomyces pombe

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Cyberlindnera jadinii
NADP+
-
 Neurospora crassa
NADP+
-
 Ovis aries
NADP+ requires NADP+ obligatorily for activity Schizosaccharomyces pombe