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Literature summary for 1.1.1.44 extracted from
- Neurospora 6-phosphogluconate dehydrogenase. I. Purification and characterization of the wild type enzyme (1973), J. Biol. Chem., 248, 3535-3544.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 1,6-diphosphate | - |
Neurospora crassa |  |
| D-fructose 1,6-diphosphate | - |
Ovis aries | |
| iodoacetamide | - |
Neurospora crassa | |
| NADPH | - |
Neurospora crassa | |
| pyridoxal 5'-phosphate | photooxidation | Cyberlindnera jadinii | |
| pyridoxal 5'-phosphate | photooxidation | Neurospora crassa | |
| Rose bengal | photooxidation | Neurospora crassa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
6-phosphogluconate | - |
Neurospora crassa | |
| 0.025 | - |
NADP+ | source of enzyme: peak I | Neurospora crassa | |
| 0.03 | - |
NADP+ | source of enzyme: whole cells | Neurospora crassa | |
| 0.033 | - |
NADP+ | source of enzyme: peak II | Neurospora crassa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
2 * 57000, SDS-PAGE | Neurospora crassa |
| 110000 | 120000 | gel filtration | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-phospho-D-gluconate + NADP+ | Cyberlindnera jadinii | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 6-phospho-D-gluconate + NADP+ | Neurospora crassa | enzyme specific for the oxidative pentose phosphate pathway | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 6-phospho-D-gluconate + NADP+ | Ovis aries | enzyme specific for the oxidative pentose phosphate pathway | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyberlindnera jadinii | - |
- |
- |
| Neurospora crassa | - |
- |
- |
| Ovis aries | - |
sheep | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neurospora crassa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30.8 | - |
- |
Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-phospho-D-gluconate + NADP+ | - |
Cyberlindnera jadinii | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 6-phospho-D-gluconate + NADP+ | - |
Neurospora crassa | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 6-phospho-D-gluconate + NADP+ | - |
Ovis aries | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 6-phospho-D-gluconate + NADP+ | enzyme specific for the oxidative pentose phosphate pathway | Neurospora crassa | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 6-phospho-D-gluconate + NADP+ | enzyme specific for the oxidative pentose phosphate pathway | Ovis aries | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Cyberlindnera jadinii |
| dimer | - |
Ovis aries |
| dimer | 2 * 57000, SDS-PAGE | Neurospora crassa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable for at least 120 min | Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 9 | - |
Neurospora crassa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 3-acetylpyridine-NADP+ | - |
Neurospora crassa | |
| deamino-NADP+ | - |
Neurospora crassa | |
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