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Literature summary for 1.1.1.435 extracted from

  • Hobbs, M.E.; Vetting, M.; Williams, H.J.; Narindoshvili, T.; Kebodeaux, D.M.; Hillerich, B.; Seidel, R.D.; Almo, S.C.; Raushel, F.M.
    Discovery of an L-fucono-1,5-lactonase from cog3618 of the amidohydrolase superfamily (2013), Biochemistry, 52, 239-253.
    View publication on PubMed
Search for more literature for 1.1.1.435

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Burkholderia multivorans

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.2 M calcium acetate, 10% (w/v) PEG 8000, 0.1 M HEPES pH 7.5 for the unliganded enzyme, or 0.2 M MgCl2, 20% (w/v) PEG8000, 0.1 M Tris pH 8.5 for enzyme in complex with NADP+ and L-fucose Burkholderia multivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 NADP+ with beta-L-fucose as cosubstrate, at pH 8.0 and 30°C Burkholderia multivorans
0.0062
-
 beta-L-fucose with NADP+ as cosubstrate, at pH 8.0 and 30°C Burkholderia multivorans
0.028
-
 NAD+ with beta-L-fucose as cosubstrate, at pH 8.0 and 30°C Burkholderia multivorans
0.094
-
 D-arabinose with NADP+ as cosubstrate, at pH 8.0 and 30°C Burkholderia multivorans
0.285
-
 L-galactose with NADP+ as cosubstrate, at pH 8.0 and 30°C Burkholderia multivorans
0.786
-
 4-deoxy-L-fucose with NADP+ as cosubstrate, at pH 8.0 and 30°C Burkholderia multivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-L-fucopyranose + NADP+ Burkholderia multivorans
-
 L-fucono-1,5-lactone + NADPH + H+
-
 ?
beta-L-fucopyranose + NADP+ Burkholderia multivorans ATCC 17616
-
 L-fucono-1,5-lactone + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Burkholderia multivorans A0A0H3KNE7
-
-
Burkholderia multivorans ATCC 17616 A0A0H3KNE7
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Superdex 200 gel filtration, and ResourceQ column chromatography Burkholderia multivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-deoxy-L-fucose + NADP+
-
 Burkholderia multivorans 4-deoxy-L-fucono-1,5-lactone + NADPH + H+
-
 ?
4-deoxy-L-fucose + NADP+
-
 Burkholderia multivorans ATCC 17616 4-deoxy-L-fucono-1,5-lactone + NADPH + H+
-
 ?
beta-L-fucopyranose + NADP+
-
 Burkholderia multivorans L-fucono-1,5-lactone + NADPH + H+
-
 ?
beta-L-fucopyranose + NADP+
-
 Burkholderia multivorans ATCC 17616 L-fucono-1,5-lactone + NADPH + H+
-
 ?
beta-L-fucose + NAD+ the enzyme has preference for NADP+ over NAD+ Burkholderia multivorans L-fucono-1,5-lactone + NADH + H+
-
 ?
beta-L-fucose + NAD+ the enzyme has preference for NADP+ over NAD+ Burkholderia multivorans ATCC 17616 L-fucono-1,5-lactone + NADH + H+
-
 ?
beta-L-fucose + NADP+ beta-L-fucose is the best substrate. The enzyme has preference for NADP+ over NAD+ Burkholderia multivorans L-fucono-1,5-lactone + NADPH + H+
-
 ?
beta-L-fucose + NADP+ beta-L-fucose is the best substrate. The enzyme has preference for NADP+ over NAD+ Burkholderia multivorans ATCC 17616 L-fucono-1,5-lactone + NADPH + H+
-
 ?
D-arabinose + NADP+
-
 Burkholderia multivorans D-arabinono-1,5-lactone + NADPH + H+
-
 ?
L-galactose + NADP+
-
 Burkholderia multivorans L-galactono-1,5-lactone + NADPH + H+
-
 ?
L-galactose + NADP+
-
 Burkholderia multivorans ATCC 17616 L-galactono-1,5-lactone + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
homotetramer x-ray crystallography Burkholderia multivorans

Synonyms

Synonyms Comment Organism
BmulJ_04919
-
 Burkholderia multivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
 NAD+ with beta-L-fucose as cosubstrate, at pH 8.0 and 30°C Burkholderia multivorans
10
-
 beta-L-fucose with NADP+ as cosubstrate, at pH 8.0 and 30°C Burkholderia multivorans
11
-
 L-galactose with NADP+ as cosubstrate, at pH 8.0 and 30°C Burkholderia multivorans
11
-
 NADP+ with beta-L-fucose as cosubstrate, at pH 8.0 and 30°C Burkholderia multivorans
21
-
 D-arabinose with NADP+ as cosubstrate, at pH 8.0 and 30°C Burkholderia multivorans
26
-
 4-deoxy-L-fucose with NADP+ as cosubstrate, at pH 8.0 and 30°C Burkholderia multivorans

Cofactor

Cofactor Comment Organism Structure
NADP+ the enzyme has preference for NADP+ over NAD+ Burkholderia multivorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
37
-
 L-galactose with NADP+ as cosubstrate, at pH 8.0 and 30°C Burkholderia multivorans
40
-
 4-deoxy-L-fucose with NADP+ as cosubstrate, at pH 8.0 and 30°C Burkholderia multivorans
230
-
 D-arabinose with NADP+ as cosubstrate, at pH 8.0 and 30°C Burkholderia multivorans
530
-
 NAD+ with beta-L-fucose as cosubstrate, at pH 8.0 and 30°C Burkholderia multivorans
1500
-
 beta-L-fucose with NADP+ as cosubstrate, at pH 8.0 and 30°C Burkholderia multivorans
3000
-
 NADP+ with beta-L-fucose as cosubstrate, at pH 8.0 and 30°C Burkholderia multivorans