Cloned (Comment) | Organism |
---|---|
gene XYL1.1, recombinant expression in Aureobasidium pullulans strain CBS 110374 | Spathaspora passalidarum |
gene XYL1.2, recombinant expression in Aureobasidium pullulans strain CBS 110374 | Spathaspora passalidarum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the combinatorial expression of two xylose reductase (XR) genes and two xylitol dehydrogenase (XDH) genes from Spathaspora passalidarum and the heterologous expression of the Piromyces sp. xylose isomerase (XI) gene are induced in Aureobasidium pullulans strain CBS 110374. Overexpression of XYL1.2 (encoding XR) and XYL2.2 (encoding XDH) is the most beneficial for xylose utilization, resulting in a 17.76% increase in consumed xylose compared with the parent strain, whereas the introduction of the Piromyces sp. XI pathway fails to enhance xylose utilization efficiency. Construction of knock-in mutants, method, evaluation of internal redox state, xylose utilization and cell growth of the recombinant strains, pH 5.8, 28°C, comparison the fermentation abilities of the parent strain and the recombinant strains using several carbon sources, overview | Spathaspora passalidarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADPH + H+ | Spathaspora passalidarum | - |
xylitol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spathaspora passalidarum | A0A0S2PJI4 | - |
- |
Spathaspora passalidarum | S4WCW2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADPH + H+ | - |
Spathaspora passalidarum | xylitol + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
XYL1.1 | - |
Spathaspora passalidarum |
XYL1.2 | - |
Spathaspora passalidarum |
xylose reductase | - |
Spathaspora passalidarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
in vivo assay at, recombinant enzyme | Spathaspora passalidarum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Spathaspora passalidarum | |
NADPH | - |
Spathaspora passalidarum |