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Literature summary for 1.1.1.43 extracted from

  • Ben-Bassat, A.; Goldberg, I.
    Purification and properties of glucose-6-phosphate dehydrogenase (NADP+/NAD+) and 6-phosphogluconate dehydrogenase (NADP+/NAD+) from methanol-grown Pseudomonas C (1980), Biochim. Biophys. Acta, 611, 1-10.
    View publication on PubMed
Search for more literature for 1.1.1.43

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
 Enterococcus faecalis
ATP with NAD+ as coenzyme, with NADP+ as coenzyme activity is less affected Pseudomonas sp.
NADH
-
 Pseudomonas sp.
NADPH
-
 Burkholderia cepacia
NADPH
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 NADP+
-
 [Candida] boidinii
0.02
-
 NADP+
-
 Pseudomonas sp.
0.04
-
 NAD+
-
 Pseudomonas sp.
0.1
-
 6-phospho-D-gluconate
-
 Pseudomonas sp.
0.5
-
 NAD+
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2 mM required for maximum activity Pseudomonas sp.
Mg2+ 20 mM required for maximum activity [Candida] boidinii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 4 * 55000, SDS-PAGE Pseudomonas sp.
110000
-
-
 [Candida] boidinii
220000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-phospho-D-gluconate + NAD(P)+ Pseudomonas sp.
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
6-phospho-D-gluconate + NAD(P)+ Enterococcus faecalis
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
6-phospho-D-gluconate + NAD(P)+ [Candida] boidinii
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
6-phospho-D-gluconate + NAD(P)+ Burkholderia cepacia
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
6-phospho-D-gluconate + NAD(P)+ Pseudomonas sp. C
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
6-phospho-D-gluconate + NAD(P)+ Pseudomonas sp. W5
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
 type I
-
Enterococcus faecalis
-
 type I
-
Pseudomonas sp.
-
 C
-
Pseudomonas sp.
-
 W5
-
Pseudomonas sp. C
-
 C
-
Pseudomonas sp. W5
-
 W5
-
[Candida] boidinii
-
 methanol utilizing yeast
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.
-
 [Candida] boidinii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
39.7
-
-
 [Candida] boidinii
56.4
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NAD(P)+
-
 Pseudomonas sp. 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
6-phospho-D-gluconate + NAD(P)+
-
 Enterococcus faecalis 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
6-phospho-D-gluconate + NAD(P)+
-
 [Candida] boidinii 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
6-phospho-D-gluconate + NAD(P)+
-
 Burkholderia cepacia 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
6-phospho-D-gluconate + NAD(P)+
-
 Pseudomonas sp. C 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
6-phospho-D-gluconate + NAD(P)+
-
 Pseudomonas sp. W5 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
D-glucose-6-phosphate + NAD+
-
 Pseudomonas sp. 2-dehydro-6-phospho-D-glucose + NADH
-
 ?
D-glucose-6-phosphate + NAD+
-
 Pseudomonas sp. C 2-dehydro-6-phospho-D-glucose + NADH
-
 ?
D-glucose-6-phosphate + NAD+
-
 Pseudomonas sp. W5 2-dehydro-6-phospho-D-glucose + NADH
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 55000, SDS-PAGE Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 [Candida] boidinii
8 9
-
 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Burkholderia cepacia
NAD+ dual specificity for both NAD+ and NADP+ Pseudomonas sp.
NADP+
-
 Burkholderia cepacia
NADP+ dual specificity for both NAD+ and NADP+ Pseudomonas sp.