BRENDA - Enzyme Database
show all sequences of 1.1.1.423

Biodegradation of ephedrine isomers by Arthrobacter sp. strain TS-15 discovery of novel ephedrine and pseudoephedrine dehydrogenases

Shanati, T.; Ansorge-Schumacher, M.B.; Appl. Environ. Microbiol. 86, e02487-19 (2020)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
(R,R)-(-)-pseudoephedrine
higher substrate inhibition
Arthrobacter sp. TS-15
(R,S)-(-)-ephedrine
substrate inhibition
Arthrobacter sp. TS-15
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
NAD+
pH 7.8, 25°C
Arthrobacter sp. TS-15
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(-)-(1R,2S)-ephedrine + NAD+
Arthrobacter sp. TS-15
-
(S)-2-methylamino-1-phenylpropan-1-one + NADH + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Arthrobacter sp. TS-15
A0A545BBR2
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(-)-(1R,2S)-ephedrine + NAD+
-
755862
Arthrobacter sp. TS-15
(S)-2-methylamino-1-phenylpropan-1-one + NADH + H+
-
-
-
?
(-)-(1R,2S)-ephedrine + NAD+
the enzyme exhibits a strict enantioselectivity for the oxidation of (-)-(1R,2S)-ephedrine and (R,R)-(-)-pseudoephedrine
755862
Arthrobacter sp. TS-15
(S)-2-methylamino-1-phenylpropan-1-one + NADH + H+
-
-
-
?
(R,R)-(-)-pseudoephedrine + NAD+
the enzyme exhibits a strict enantioselectivity for the oxidation of (-)-(1R,2S)-ephedrine and (R,R)-(-)-pseudoephedrine
755862
Arthrobacter sp. TS-15
? + NADH + H+
-
-
-
?
additional information
no activity on (S,N)-(+)-(pseudo)ephedrine or (R,N)-(-)-(pseudo)ephedrine
755862
Arthrobacter sp. TS-15
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
EDH
-
Arthrobacter sp. TS-15
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Arthrobacter sp. TS-15
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.78
-
(R,R)-(-)-pseudoephedrine
pH 7.8, 25°C
Arthrobacter sp. TS-15
4.61
-
(R,S)-(-)-ephedrine
pH 7.8, 25°C
Arthrobacter sp. TS-15
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Arthrobacter sp. TS-15
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(R,R)-(-)-pseudoephedrine
higher substrate inhibition
Arthrobacter sp. TS-15
(R,S)-(-)-ephedrine
substrate inhibition
Arthrobacter sp. TS-15
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.78
-
(R,R)-(-)-pseudoephedrine
pH 7.8, 25°C
Arthrobacter sp. TS-15
4.61
-
(R,S)-(-)-ephedrine
pH 7.8, 25°C
Arthrobacter sp. TS-15
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
NAD+
pH 7.8, 25°C
Arthrobacter sp. TS-15
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(-)-(1R,2S)-ephedrine + NAD+
Arthrobacter sp. TS-15
-
(S)-2-methylamino-1-phenylpropan-1-one + NADH + H+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(-)-(1R,2S)-ephedrine + NAD+
-
755862
Arthrobacter sp. TS-15
(S)-2-methylamino-1-phenylpropan-1-one + NADH + H+
-
-
-
?
(-)-(1R,2S)-ephedrine + NAD+
the enzyme exhibits a strict enantioselectivity for the oxidation of (-)-(1R,2S)-ephedrine and (R,R)-(-)-pseudoephedrine
755862
Arthrobacter sp. TS-15
(S)-2-methylamino-1-phenylpropan-1-one + NADH + H+
-
-
-
?
(R,R)-(-)-pseudoephedrine + NAD+
the enzyme exhibits a strict enantioselectivity for the oxidation of (-)-(1R,2S)-ephedrine and (R,R)-(-)-pseudoephedrine
755862
Arthrobacter sp. TS-15
? + NADH + H+
-
-
-
?
additional information
no activity on (S,N)-(+)-(pseudo)ephedrine or (R,N)-(-)-(pseudo)ephedrine
755862
Arthrobacter sp. TS-15
?
-
-
-
-
Other publictions for EC 1.1.1.423
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
755862
Shanati
Biodegradation of ephedrine i ...
Arthrobacter sp. TS-15
Appl. Environ. Microbiol.
86
e02487-19
2020
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-
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2
1
-
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1
-
1
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-
-
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4
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
-
-
2
2
1
-
-
-
1
-
-
-
-
-
-
-
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4
-
-
-
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-
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-
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-
752351
Shanati
-
Two enantiocomplementary ephe ...
Arthrobacter sp. TS-15
ACS Catal.
9
6202-6211
2019
-
3
1
-
-
-
6
15
-
-
2
1
-
1
-
-
1
-
-
-
-
-
52
3
1
1
2
3
17
1
-
2
2
6
-
-
-
3
1
2
-
-
-
-
6
6
15
-
-
2
1
-
-
-
1
-
-
-
-
52
3
1
2
3
17
1
-
2
-
-
-
-
-
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