Application | Comment | Organism |
---|---|---|
industry | the wide substrate spectrum of these dehydrogenases, combined with their regio- and enantioselectivity, suggests a high potential for the industrial production of valuable chiral compounds | Arthrobacter sp. TS-15 |
synthesis | the wide substrate spectrum of these dehydrogenases, combined with their regio- and enantioselectivity, suggests a high potential for the industrial production of valuable chiral compounds | Arthrobacter sp. TS-15 |
synthesis | the enzyme catalyzes the oxidation of an isomer of ephedrine and the regio- and enantioselective reduction of sterically demanding substrate 1-phenyl-1,2-propanedione to give (R)-phenylacetylcarbinol | Arthrobacter sp. TS-15 |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli T7 shuffle (DE3) | Arthrobacter sp. TS-15 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-Phenyl-1,3-butanedione | - |
Arthrobacter sp. TS-15 | |
2-aminoacetophenone | - |
Arthrobacter sp. TS-15 | |
2-chloro-1-phenyl-1-propane | - |
Arthrobacter sp. TS-15 | |
2-chloro-1-phenyl-1-propanone | - |
Arthrobacter sp. TS-15 | |
ethyl 2-chlorobenzoylacetate | - |
Arthrobacter sp. TS-15 | |
methyl benzoylformate | - |
Arthrobacter sp. TS-15 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
1-phenyl-1-propanone | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
0.02 | - |
2-chloro-1-phenyl-1-propanone | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
0.02 | - |
2-chloro-1-phenyl-1-propane | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
0.06 | - |
1-phenyl-1,2-propanedione | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
0.06 | - |
ethyl 2-chlorobenzoylacetate | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
0.07 | - |
NADH | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
0.1 | - |
1,2-naphthoquinone | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
0.17 | - |
4-chlorobenzil | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
0.19 | - |
methyl benzoylformate | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
0.2 | - |
1-Phenyl-1,3-butanedione | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
0.51 | - |
isatin | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
0.6 | - |
2-aminoacetophenone | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
2.29 | - |
1,2-indanedione | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
2.29 | - |
1,2-indandione | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
5.67 | - |
Phenylglyoxal | pH 7.5, 25°C | Arthrobacter sp. TS-15 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25930 | - |
calculated from sequence | Arthrobacter sp. TS-15 |
27000 | - |
SDS-PAGE | Arthrobacter sp. TS-15 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-(1R,2S)-ephedrine + NAD+ | Arthrobacter sp. TS-15 | - |
(S)-2-methylamino-1-phenylpropan-1-one + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. TS-15 | A0A545BBR2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arthrobacter sp. TS-15 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-(1R,2S)-ephedrine + NAD+ | - |
Arthrobacter sp. TS-15 | (S)-2-methylamino-1-phenylpropan-1-one + NADH + H+ | - |
? | |
(-)-(1R,2S)-ephedrine + NAD+ | the enzyme acts on a broad range of different aryl-alkyl ketones, such as haloketones, ketoamines, diketones, and ketoesters. It accepts various types of aryl groups including phenyl-, pyridyl-, thienyl-, and furyl-rings, but the presence of an aromatic ring is essential for the activity. In addition, the presence of a functional group on the alkyl chain, such as an amine, a halogen, or a ketone, is also crucial. The enzyme exhibits a strict Prelog enantioselectivity. When acting on diketones, it catalyses the reduction of only the keto group closest to the ring, with no further reduction to the diol. (-)-(1R,2S)-ephedrine i.e. (1R,2S)-1-phenyl-1-hydroxy-2-methylaminopropane. (S)-2-methylamino-1-phenylpropan-1-one i.e. (S)-methcathinone | Arthrobacter sp. TS-15 | (S)-2-methylamino-1-phenylpropan-1-one + NADH + H+ | - |
? | |
(4-chlorophenyl)-2-pyridinylmethanone + NAD+ | specifc activity: 1.74 U/mg. Product: 70% (S) enantiomeric excess | Arthrobacter sp. TS-15 | (S)-(4-chlorophenyl)(pyridin-2-yl)methanol + NADH + H+ | - |
? | |
(4-chlorophenyl)-2-pyridinylmethanone + NADH + H+ | 2.7% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 70% enantiomeric excess | Arthrobacter sp. TS-15 | (R)-(4-chlorophenyl)(pyridin-2-yl)methanol + NAD+ | - |
? | |
1,2-indandione + NAD+ | specifc 50.05 U/mg | Arthrobacter sp. TS-15 | ? + NADH + H+ | - |
? | |
1,2-indanedione + NADH + H+ | 78.1% of the activity as compared to 1-phenyl-1,2-propanedione | Arthrobacter sp. TS-15 | 1-hydroxy-1,3-dihydro-2H-inden-2-one + NAD+ | - |
? | |
1,2-naphthoquinone + NAD+ | specifc activity: 13.89 U/mg | Arthrobacter sp. TS-15 | ? + NADH + H+ | - |
? | |
1,2-naphthoquinone + NADH + H+ | 21.7% of the activity as compared to 1-phenyl-1,2-propanedione | Arthrobacter sp. TS-15 | 1-hydroxynaphthalen-2(1H)-one + NAD+ | - |
? | |
1-phenyl-1,2-propanedione + NAD+ | specifc activity: 64.02 U/mg. Product: 99% (R) enantiomeric excess | Arthrobacter sp. TS-15 | (R)-phenylacetylcarbinol + NADH + H+ | - |
? | |
1-phenyl-1,2-propanedione + NADH + H+ | substrate with highest activity. Full conversion and enantiomeric excess of more than 99% | Arthrobacter sp. TS-15 | (R)-phenylacetylcarbinol + NAD+ | - |
? | |
1-phenyl-1,3-butanedione + NAD+ | specifc activity: 13.64 U/mg. Product: 98% (R) enantiomeric excess | Arthrobacter sp. TS-15 | (4R)-4-hydroxy-4-phenylbutan-2-one + NADH + H+ | - |
? | |
1-phenyl-1,3-butanedione + NADH + H+ | 21.3% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 98% enantiomeric excess | Arthrobacter sp. TS-15 | (4R)-4-hydroxy-4-phenylbutan-2-one + NAD+ | - |
? | |
1-phenyl-1-propanone + NAD+ | specifc activity: 1.55 U/mg. Product: 99% (R) enantiomeric excess | Arthrobacter sp. TS-15 | (1R)-1-phenylpropan-1-ol + NADH + H+ | - |
? | |
1-phenyl-1-propanone + NADH + H+ | 2.4% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess | Arthrobacter sp. TS-15 | (1R)-1-phenylpropan-1-ol + NAD+ | - |
? | |
2,2'-dichlorobenzil + NAD+ | specifc activity: 27.91 U/mg. Product: 94% (R) enantiomeric excess | Arthrobacter sp. TS-15 | (2R)-1,2-bis(2-chlorophenyl)-2-hydroxyethan-1-one + NADH + H+ | - |
? | |
2,2'-dichlorobenzil + NADH + H+ | 43.6% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 4% enantiomeric excess | Arthrobacter sp. TS-15 | (2R)-1,2-bis(2-chlorophenyl)-2-hydroxyethan-1-one + NAD+ | - |
? | |
2,2'-furil + NAD+ | specifc activity: 19.95 U/mg. Product: 99% (R) enantiomeric excess | Arthrobacter sp. TS-15 | (2R)-1,2-di(furan-2-yl)-2-hydroxyethan + NADH + H+ | - |
? | |
2,2'-furil + NADH + H+ | 20% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess | Arthrobacter sp. TS-15 | (2R)-1,2-di(furan-2-yl)-2-hydroxyethan-1-one + NAD+ | - |
? | |
2,2'-thenil + NAD+ | specifc activity: 46.77 U/mg. Product: 99% (R) enantiomeric excess | Arthrobacter sp. TS-15 | (2R)-2-hydroxy-1,2-di(thiophen-2-yl)ethan-1-one + NADH + H+ | - |
? | |
2,2'-thenil + NADH + H+ | 46.8% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess | Arthrobacter sp. TS-15 | (2R)-2-hydroxy-1,2-di(thiophen-2-yl)ethan-1-one + NAD+ | - |
? | |
2-aminoacetophenone + NAD+ | specifc activity: 25.79 U/mg. Product: 99% (R) enantiomeric excess | Arthrobacter sp. TS-15 | (1R)-2-amino-1-phenylethan-1-ol + NADH + H+ | - |
? | |
2-aminoacetophenone + NADH + H+ | 40.3% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess | Arthrobacter sp. TS-15 | (1R)-2-amino-1-phenylethan-1-ol + NAD+ | - |
? | |
2-bromo-1-(3-chlorophenyl)-1-propanone + NAD+ | specifc activity: 5.1 U/mg | Arthrobacter sp. TS-15 | (R,S)-2-bromo-1-(3-chlorophenyl)propan-1-ol + NADH + H+ | - |
? | |
2-bromo-1-(3-chlorophenyl)-1-propanone + NADH + H+ | 7.9% of the activity as compared to 1-phenyl-1,2-propanedione | Arthrobacter sp. TS-15 | 2-bromo-1-(3-chlorophenyl)propan-1-ol + NAD+ | - |
? | |
2-bromoacetophenone + NAD+ | specifc activity: 9.44 U/mg. Product: 85% (R) enantiomeric excess | Arthrobacter sp. TS-15 | (1R)-2-bromo-1-phenylethan-1-ol + NADH + H+ | - |
? | |
2-bromoacetophenone + NADH + H+ | 14.8% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 85% enantiomeric excess | Arthrobacter sp. TS-15 | (1R)-2-bromo-1-phenylethan-1-ol + NAD+ | - |
? | |
2-chloro-1-phenyl-1-propane + NAD+ | specifc activity: 8.0 U/mg | Arthrobacter sp. TS-15 | (R,S)-2-chloro-1-phenylpropan-1-ol + NADH + H+ | - |
? | |
2-chloro-1-phenyl-1-propanone + NADH + H+ | 12.5% of the activity as compared to 1-phenyl-1,2-propanedione | Arthrobacter sp. TS-15 | 2-chloro-1-phenyl-1-propan-1-ol + NAD+ | - |
? | |
2-chloroacetophenone + NAD+ | specifc activity: 8.71 U/mg. Product: 98% (R) enantiomeric excess | Arthrobacter sp. TS-15 | (1R)-2-chloro-1-phenylethan-1-ol + NADH + H+ | - |
? | |
2-chloroacetophenone + NADH + H+ | 13.6% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 98% enantiomeric excess | Arthrobacter sp. TS-15 | (1R)-2-chloro-1-phenylethan-1-ol + NAD+ | - |
? | |
2-thenoylacetonitrile + NAD+ | specifc activity: 6.87 U/mg. Product: 99% (R) enantiomeric excess | Arthrobacter sp. TS-15 | (3R)-3-hydroxy-3-(thiophen-2-yl)propanenitril + NADH + H+ | - |
? | |
2-thenoylacetonitrile + NADH + H+ | 6.87% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess | Arthrobacter sp. TS-15 | (2R)-hydroxy(thiophen-2-yl)acetonitrile + NAD+ | - |
? | |
3-chloropropiophenone + NAD+ | specifc activity: 4.45 U/mg. Product: 99% (R) enantiomeric excess | Arthrobacter sp. TS-15 | (1R)-3-chloro-1-phenylpropan-1-ol + NADH + H+ | - |
? | |
3-chloropropiophenone + NADH + H+ | 6.9% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess | Arthrobacter sp. TS-15 | (1R)-3-chloro-1-phenylpropan-1-ol + NAD+ | - |
? | |
4,4'-difluorobenzil + NAD+ | specifc activity: 48.2 U/mg. Product: 99% (R) enantiomeric excess | Arthrobacter sp. TS-15 | (2R)-1,2-bis(4-fluorophenyl)-2-hydroxyethan-1-one + NADH + H+ | - |
? | |
4,4'-difluorobenzil + NADH + H+ | 75.3% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess | Arthrobacter sp. TS-15 | (2R)-1,2-bis(4-fluorophenyl)-2-hydroxyethan-1-one + NAD+ | - |
? | |
4,4'-dimethylbenzil + NAD+ | specifc activity: 3.12 U/mg. Product: 99% (R) enantiomeric excess | Arthrobacter sp. TS-15 | (2R)-2-hydroxy-1,2-bis(4-methylphenyl)ethan-1-one + NADH + H+ | - |
? | |
4,4'-dimethylbenzil + NADH + H+ | 4.8% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess | Arthrobacter sp. TS-15 | (2R)-1,2-bis(4-methylphenyl)-2-hydroxyethan-1-one + NAD+ | - |
? | |
4-chlorobenzil + NAD+ | specifc activity: 6.5 U/mg. Product: 95% (R) enantiomeric excess | Arthrobacter sp. TS-15 | ? + NADH + H+ | - |
? | |
4-chlorobenzil + NADH + H+ | 10.2% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 58% enantiomeric excess | Arthrobacter sp. TS-15 | ? + NAD+ | - |
? | |
benzil + NAD+ | specifc activity: 47.06 U/mg. Product: 99% enantiomeric (R) excess | Arthrobacter sp. TS-15 | (2R)-2-hydroxy-1,2-diphenylethan-1-one + NADH + H+ | - |
? | |
benzil + NADH + H+ | 73.5% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess | Arthrobacter sp. TS-15 | (2R)-2-hydroxy-1,2-diphenylethan-1-one + NAD+ | ? | ? | |
ethyl 2-chlorobenzoylacetate + NAD+ | specifc activity: 35.21 U/mg | Arthrobacter sp. TS-15 | ethyl (R,S)-2-chloro-3-hydroxy-3-phenylpropanoate + NADH + H+ | - |
? | |
ethyl 2-chlorobenzoylacetate + NADH + H+ | 54.9% of the activity as compared to 1-phenyl-1,2-propanedione | Arthrobacter sp. TS-15 | ethyl 2-chloro-3-hydroxy-3-phenylpropanoate + NAD+ | - |
? | |
isatin + NAD+ | specifc activity: 7.84 U/mg. Product: 52% (R) enantiomeric excess | Arthrobacter sp. TS-15 | ? + NADH + H+ | - |
? | |
isatin + NADH + H+ | 12.25% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 52% enantiomeric excess | Arthrobacter sp. TS-15 | (3R)-3-hydroxy-1,3-dihydro-2H-indol-2-one + NAD+ | - |
? | |
methyl benzoylformate + NAD+ | specifc activity: 46.56 U/mg. Product: 98% (R) enantiomeric excess | Arthrobacter sp. TS-15 | methyl (2R)-hydroxy(phenyl)acetate + NADH + H+ | - |
? | |
methyl benzoylformate + NADH + H+ | 72.7% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 98% enantiomeric excess | Arthrobacter sp. TS-15 | methyl (2S)-hydroxy(phenyl)acetate + NAD+ | - |
? | |
phenyl-2-pyridinylmethanone + NAD+ | specifc activity: 2.81 U/mg. Product: 24% (R) enantiomeric excess | Arthrobacter sp. TS-15 | (R)-phenyl(pyridin-2-yl)methanol + NADH + H+ | - |
? | |
phenyl-2-pyridinylmethanone + NADH + H+ | 4.4% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 24% enantiomeric excess | Arthrobacter sp. TS-15 | (R)-phenyl(pyridin-2-yl)methanol + NAD+ | - |
? | |
phenylglyoxal + NAD+ | specifc activity: 12.1 U/mg. Product: 99% (R) enantiomeric excess | Arthrobacter sp. TS-15 | (2R)-hydroxy(phenyl)acetaldehyde + NADH + H+ | - |
? | |
phenylglyoxal + NADH + H+ | 18.9% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess | Arthrobacter sp. TS-15 | (2R)-hydroxy(phenyl)acetaldehyde + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 4 * 27000, SDS-PAGE, the smallest oligomeric state seems to be a dimer, the predominant oligomeric state is the tetramer | Arthrobacter sp. TS-15 |
tetramer | 4 * 27000, SDS-PAGE, the smallest oligomeric state seems to be a dimer, the predominant oligomeric state is the tetramer | Arthrobacter sp. TS-15 |
tetramer | 4 * 27000, the enzyme tends to form higher oligomeric states, up to a hexadecamer, SDS-PAGE | Arthrobacter sp. TS-15 |
Synonyms | Comment | Organism |
---|---|---|
EDH | - |
Arthrobacter sp. TS-15 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Arthrobacter sp. TS-15 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 75 | 55°C: about 40% of maximal activity, 75°C: about 75% of maximal activity | Arthrobacter sp. TS-15 |
65 | 75 | 65°C: about 55% of maximal activity, 75°C: about 75% of maximal activity | Arthrobacter sp. TS-15 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | - |
half-life: 2.6 h | Arthrobacter sp. TS-15 |
25 | - |
half-life: 38.3 h | Arthrobacter sp. TS-15 |
40 | - |
half-life: 82.8 h | Arthrobacter sp. TS-15 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.77 | - |
1-phenyl-1-propanone | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
2.54 | - |
2-bromo-1-(3-chlorophenyl)-1-propanone | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
3.48 | - |
4-chlorobenzil | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
4.36 | - |
isatin | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
7.31 | - |
2-chloro-1-phenyl-1-propanone | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
7.31 | - |
2-chloro-1-phenyl-1-propane | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
7.94 | - |
1,2-naphthoquinone | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
9.49 | - |
Phenylglyoxal | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
15.37 | - |
1-Phenyl-1,3-butanedione | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
15.74 | - |
NADH | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
27.12 | - |
2-aminoacetophenone | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
27.13 | - |
2-aminoacetophenone | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
30.43 | - |
1-phenyl-1,2-propanedione | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
31.11 | - |
1,2-indanedione | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
31.11 | - |
1,2-indandione | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
37.38 | - |
ethyl 2-chlorobenzoylacetate | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
40.05 | - |
methyl benzoylformate | pH 7.5, 25°C | Arthrobacter sp. TS-15 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Arthrobacter sp. TS-15 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | highest stability | Arthrobacter sp. TS-15 |
7.5 | 8.5 | t1/2: 34-21 h | Arthrobacter sp. TS-15 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | no apparent activity with NADP(H)+ and full catalytic activity with NAD(H). The preference for NADH makes the utilization of the enzyme an attractive for industrial applications, since NADH is more stable and less expensive than NADPH under operational reaction conditions | Arthrobacter sp. TS-15 | |
NADH | no apparent activity with NADP(H)+ and full catalytic activity with NAD(H). The preference for NADH makes the utilization of the enzyme an attractive for industrial applications, since NADH is more stable and less expensive than NADPH under operational reaction conditions | Arthrobacter sp. TS-15 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
2-chloro-1-phenyl-1-propanone | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
0.1 | - |
2-chloro-1-phenyl-1-propane | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
0.16 | - |
ethyl 2-chlorobenzoylacetate | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
0.26 | - |
1-Phenyl-1,3-butanedione | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
1.46 | - |
methyl benzoylformate | pH 7.5, 25°C | Arthrobacter sp. TS-15 | |
1.53 | - |
2-aminoacetophenone | pH 7.5, 25°C | Arthrobacter sp. TS-15 |