BRENDA - Enzyme Database
show all sequences of 1.1.1.423

Two enantiocomplementary ephedrine dehydrogenases from Arthrobacter sp. TS-15 with broad substrate specificity

Shanati, T.; Lockie, C.; Beloti, L.; Grogan, G.; Ansorge-Schumacher, M.B.; ACS Catal. 9, 6202-6211 (2019)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
industry
the wide substrate spectrum of these dehydrogenases, combined with their regio- and enantioselectivity, suggests a high potential for the industrial production of valuable chiral compounds
Arthrobacter sp. TS-15
synthesis
the wide substrate spectrum of these dehydrogenases, combined with their regio- and enantioselectivity, suggests a high potential for the industrial production of valuable chiral compounds
Arthrobacter sp. TS-15
synthesis
the enzyme catalyzes the oxidation of an isomer of ephedrine and the regio- and enantioselective reduction of sterically demanding substrate 1-phenyl-1,2-propanedione to give (R)-phenylacetylcarbinol
Arthrobacter sp. TS-15
Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli T7 shuffle (DE3)
Arthrobacter sp. TS-15
Inhibitors
Inhibitors
Commentary
Organism
Structure
1-Phenyl-1,3-butanedione
-
Arthrobacter sp. TS-15
2-aminoacetophenone
-
Arthrobacter sp. TS-15
2-chloro-1-phenyl-1-propane
-
Arthrobacter sp. TS-15
2-chloro-1-phenyl-1-propanone
-
Arthrobacter sp. TS-15
ethyl 2-chlorobenzoylacetate
-
Arthrobacter sp. TS-15
methyl benzoylformate
-
Arthrobacter sp. TS-15
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
1-phenyl-1-propanone
pH 7.5, 25C
Arthrobacter sp. TS-15
0.02
-
2-chloro-1-phenyl-1-propanone
pH 7.5, 25C
Arthrobacter sp. TS-15
0.02
-
2-chloro-1-phenyl-1-propane
pH 7.5, 25C
Arthrobacter sp. TS-15
0.06
-
1-phenyl-1,2-propanedione
pH 7.5, 25C
Arthrobacter sp. TS-15
0.06
-
ethyl 2-chlorobenzoylacetate
pH 7.5, 25C
Arthrobacter sp. TS-15
0.07
-
NADH
pH 7.5, 25C
Arthrobacter sp. TS-15
0.1
-
1,2-naphthoquinone
pH 7.5, 25C
Arthrobacter sp. TS-15
0.17
-
4-chlorobenzil
pH 7.5, 25C
Arthrobacter sp. TS-15
0.19
-
methyl benzoylformate
pH 7.5, 25C
Arthrobacter sp. TS-15
0.2
-
1-Phenyl-1,3-butanedione
pH 7.5, 25C
Arthrobacter sp. TS-15
0.51
-
isatin
pH 7.5, 25C
Arthrobacter sp. TS-15
0.6
-
2-aminoacetophenone
pH 7.5, 25C
Arthrobacter sp. TS-15
2.29
-
1,2-indanedione
pH 7.5, 25C
Arthrobacter sp. TS-15
2.29
-
1,2-indandione
pH 7.5, 25C
Arthrobacter sp. TS-15
5.67
-
Phenylglyoxal
pH 7.5, 25C
Arthrobacter sp. TS-15
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25930
-
calculated from sequence
Arthrobacter sp. TS-15
27000
-
SDS-PAGE
Arthrobacter sp. TS-15
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(-)-(1R,2S)-ephedrine + NAD+
Arthrobacter sp. TS-15
-
(S)-2-methylamino-1-phenylpropan-1-one + NADH + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Arthrobacter sp. TS-15
A0A545BBR2
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Arthrobacter sp. TS-15
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(-)-(1R,2S)-ephedrine + NAD+
-
752351
Arthrobacter sp. TS-15
(S)-2-methylamino-1-phenylpropan-1-one + NADH + H+
-
-
-
?
(-)-(1R,2S)-ephedrine + NAD+
the enzyme acts on a broad range of different aryl-alkyl ketones, such as haloketones, ketoamines, diketones, and ketoesters. It accepts various types of aryl groups including phenyl-, pyridyl-, thienyl-, and furyl-rings, but the presence of an aromatic ring is essential for the activity. In addition, the presence of a functional group on the alkyl chain, such as an amine, a halogen, or a ketone, is also crucial. The enzyme exhibits a strict Prelog enantioselectivity. When acting on diketones, it catalyses the reduction of only the keto group closest to the ring, with no further reduction to the diol. (-)-(1R,2S)-ephedrine i.e. (1R,2S)-1-phenyl-1-hydroxy-2-methylaminopropane. (S)-2-methylamino-1-phenylpropan-1-one i.e. (S)-methcathinone
752351
Arthrobacter sp. TS-15
(S)-2-methylamino-1-phenylpropan-1-one + NADH + H+
-
-
-
?
(4-chlorophenyl)-2-pyridinylmethanone + NAD+
specifc activity: 1.74 U/mg. Product: 70% (S) enantiomeric excess
752351
Arthrobacter sp. TS-15
(S)-(4-chlorophenyl)(pyridin-2-yl)methanol + NADH + H+
-
-
-
?
(4-chlorophenyl)-2-pyridinylmethanone + NADH + H+
2.7% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 70% enantiomeric excess
752351
Arthrobacter sp. TS-15
(R)-(4-chlorophenyl)(pyridin-2-yl)methanol + NAD+
-
-
-
?
1,2-indandione + NAD+
specifc 50.05 U/mg
752351
Arthrobacter sp. TS-15
? + NADH + H+
-
-
-
?
1,2-indanedione + NADH + H+
78.1% of the activity as compared to 1-phenyl-1,2-propanedione
752351
Arthrobacter sp. TS-15
1-hydroxy-1,3-dihydro-2H-inden-2-one + NAD+
-
-
-
?
1,2-naphthoquinone + NAD+
specifc activity: 13.89 U/mg
752351
Arthrobacter sp. TS-15
? + NADH + H+
-
-
-
?
1,2-naphthoquinone + NADH + H+
21.7% of the activity as compared to 1-phenyl-1,2-propanedione
752351
Arthrobacter sp. TS-15
1-hydroxynaphthalen-2(1H)-one + NAD+
-
-
-
?
1-phenyl-1,2-propanedione + NAD+
specifc activity: 64.02 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(R)-phenylacetylcarbinol + NADH + H+
-
-
-
?
1-phenyl-1,2-propanedione + NADH + H+
substrate with highest activity. Full conversion and enantiomeric excess of more than 99%
752351
Arthrobacter sp. TS-15
(R)-phenylacetylcarbinol + NAD+
-
-
-
?
1-phenyl-1,3-butanedione + NAD+
specifc activity: 13.64 U/mg. Product: 98% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(4R)-4-hydroxy-4-phenylbutan-2-one + NADH + H+
-
-
-
?
1-phenyl-1,3-butanedione + NADH + H+
21.3% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 98% enantiomeric excess
752351
Arthrobacter sp. TS-15
(4R)-4-hydroxy-4-phenylbutan-2-one + NAD+
-
-
-
?
1-phenyl-1-propanone + NAD+
specifc activity: 1.55 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-1-phenylpropan-1-ol + NADH + H+
-
-
-
?
1-phenyl-1-propanone + NADH + H+
2.4% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-1-phenylpropan-1-ol + NAD+
-
-
-
?
2,2'-dichlorobenzil + NAD+
specifc activity: 27.91 U/mg. Product: 94% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-1,2-bis(2-chlorophenyl)-2-hydroxyethan-1-one + NADH + H+
-
-
-
?
2,2'-dichlorobenzil + NADH + H+
43.6% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 4% enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-1,2-bis(2-chlorophenyl)-2-hydroxyethan-1-one + NAD+
-
-
-
?
2,2'-furil + NAD+
specifc activity: 19.95 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-1,2-di(furan-2-yl)-2-hydroxyethan + NADH + H+
-
-
-
?
2,2'-furil + NADH + H+
20% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-1,2-di(furan-2-yl)-2-hydroxyethan-1-one + NAD+
-
-
-
?
2,2'-thenil + NAD+
specifc activity: 46.77 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-2-hydroxy-1,2-di(thiophen-2-yl)ethan-1-one + NADH + H+
-
-
-
?
2,2'-thenil + NADH + H+
46.8% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-2-hydroxy-1,2-di(thiophen-2-yl)ethan-1-one + NAD+
-
-
-
?
2-aminoacetophenone + NAD+
specifc activity: 25.79 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-2-amino-1-phenylethan-1-ol + NADH + H+
-
-
-
?
2-aminoacetophenone + NADH + H+
40.3% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-2-amino-1-phenylethan-1-ol + NAD+
-
-
-
?
2-bromo-1-(3-chlorophenyl)-1-propanone + NAD+
specifc activity: 5.1 U/mg
752351
Arthrobacter sp. TS-15
(R,S)-2-bromo-1-(3-chlorophenyl)propan-1-ol + NADH + H+
-
-
-
?
2-bromo-1-(3-chlorophenyl)-1-propanone + NADH + H+
7.9% of the activity as compared to 1-phenyl-1,2-propanedione
752351
Arthrobacter sp. TS-15
2-bromo-1-(3-chlorophenyl)propan-1-ol + NAD+
-
-
-
?
2-bromoacetophenone + NAD+
specifc activity: 9.44 U/mg. Product: 85% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-2-bromo-1-phenylethan-1-ol + NADH + H+
-
-
-
?
2-bromoacetophenone + NADH + H+
14.8% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 85% enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-2-bromo-1-phenylethan-1-ol + NAD+
-
-
-
?
2-chloro-1-phenyl-1-propane + NAD+
specifc activity: 8.0 U/mg
752351
Arthrobacter sp. TS-15
(R,S)-2-chloro-1-phenylpropan-1-ol + NADH + H+
-
-
-
?
2-chloro-1-phenyl-1-propanone + NADH + H+
12.5% of the activity as compared to 1-phenyl-1,2-propanedione
752351
Arthrobacter sp. TS-15
2-chloro-1-phenyl-1-propan-1-ol + NAD+
-
-
-
?
2-chloroacetophenone + NAD+
specifc activity: 8.71 U/mg. Product: 98% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-2-chloro-1-phenylethan-1-ol + NADH + H+
-
-
-
?
2-chloroacetophenone + NADH + H+
13.6% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 98% enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-2-chloro-1-phenylethan-1-ol + NAD+
-
-
-
?
2-thenoylacetonitrile + NAD+
specifc activity: 6.87 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(3R)-3-hydroxy-3-(thiophen-2-yl)propanenitril + NADH + H+
-
-
-
?
2-thenoylacetonitrile + NADH + H+
6.87% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-hydroxy(thiophen-2-yl)acetonitrile + NAD+
-
-
-
?
3-chloropropiophenone + NAD+
specifc activity: 4.45 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-3-chloro-1-phenylpropan-1-ol + NADH + H+
-
-
-
?
3-chloropropiophenone + NADH + H+
6.9% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-3-chloro-1-phenylpropan-1-ol + NAD+
-
-
-
?
4,4'-difluorobenzil + NAD+
specifc activity: 48.2 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-1,2-bis(4-fluorophenyl)-2-hydroxyethan-1-one + NADH + H+
-
-
-
?
4,4'-difluorobenzil + NADH + H+
75.3% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-1,2-bis(4-fluorophenyl)-2-hydroxyethan-1-one + NAD+
-
-
-
?
4,4'-dimethylbenzil + NAD+
specifc activity: 3.12 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-2-hydroxy-1,2-bis(4-methylphenyl)ethan-1-one + NADH + H+
-
-
-
?
4,4'-dimethylbenzil + NADH + H+
4.8% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-1,2-bis(4-methylphenyl)-2-hydroxyethan-1-one + NAD+
-
-
-
?
4-chlorobenzil + NAD+
specifc activity: 6.5 U/mg. Product: 95% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
? + NADH + H+
-
-
-
?
4-chlorobenzil + NADH + H+
10.2% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 58% enantiomeric excess
752351
Arthrobacter sp. TS-15
? + NAD+
-
-
-
?
benzil + NAD+
specifc activity: 47.06 U/mg. Product: 99% enantiomeric (R) excess
752351
Arthrobacter sp. TS-15
(2R)-2-hydroxy-1,2-diphenylethan-1-one + NADH + H+
-
-
-
?
benzil + NADH + H+
73.5% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-2-hydroxy-1,2-diphenylethan-1-one + NADH + H+
?
-
-
?
ethyl 2-chlorobenzoylacetate + NAD+
specifc activity: 35.21 U/mg
752351
Arthrobacter sp. TS-15
ethyl (R,S)-2-chloro-3-hydroxy-3-phenylpropanoate + NADH + H+
-
-
-
?
ethyl 2-chlorobenzoylacetate + NADH + H+
54.9% of the activity as compared to 1-phenyl-1,2-propanedione
752351
Arthrobacter sp. TS-15
ethyl 2-chloro-3-hydroxy-3-phenylpropanoate + NAD+
-
-
-
?
isatin + NAD+
specifc activity: 7.84 U/mg. Product: 52% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
? + NADH + H+
-
-
-
?
isatin + NADH + H+
12.25% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 52% enantiomeric excess
752351
Arthrobacter sp. TS-15
(3R)-3-hydroxy-1,3-dihydro-2H-indol-2-one + NAD+
-
-
-
?
methyl benzoylformate + NAD+
specifc activity: 46.56 U/mg. Product: 98% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
methyl (2R)-hydroxy(phenyl)acetate + NADH + H+
-
-
-
?
methyl benzoylformate + NADH + H+
72.7% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 98% enantiomeric excess
752351
Arthrobacter sp. TS-15
methyl (2S)-hydroxy(phenyl)acetate + NAD+
-
-
-
?
phenyl-2-pyridinylmethanone + NAD+
specifc activity: 2.81 U/mg. Product: 24% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(R)-phenyl(pyridin-2-yl)methanol + NADH + H+
-
-
-
?
phenyl-2-pyridinylmethanone + NADH + H+
4.4% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 24% enantiomeric excess
752351
Arthrobacter sp. TS-15
(R)-phenyl(pyridin-2-yl)methanol + NAD+
-
-
-
?
phenylglyoxal + NAD+
specifc activity: 12.1 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-hydroxy(phenyl)acetaldehyde + NADH + H+
-
-
-
?
phenylglyoxal + NADH + H+
18.9% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-hydroxy(phenyl)acetaldehyde + NAD+
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
4 * 27000, SDS-PAGE, the smallest oligomeric state seems to be a dimer, the predominant oligomeric state is the tetramer
Arthrobacter sp. TS-15
tetramer
4 * 27000, SDS-PAGE, the smallest oligomeric state seems to be a dimer, the predominant oligomeric state is the tetramer
Arthrobacter sp. TS-15
tetramer
4 * 27000, the enzyme tends to form higher oligomeric states, up to a hexadecamer, SDS-PAGE
Arthrobacter sp. TS-15
Synonyms
Synonyms
Commentary
Organism
EDH
-
Arthrobacter sp. TS-15
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
70
-
-
Arthrobacter sp. TS-15
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
55
75
55C: about 40% of maximal activity, 75C: about 75% of maximal activity
Arthrobacter sp. TS-15
65
75
65C: about 55% of maximal activity, 75C: about 75% of maximal activity
Arthrobacter sp. TS-15
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
5
-
half-life: 2.6 h
Arthrobacter sp. TS-15
25
-
half-life: 38.3 h
Arthrobacter sp. TS-15
40
-
half-life: 82.8 h
Arthrobacter sp. TS-15
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.77
-
1-phenyl-1-propanone
pH 7.5, 25C
Arthrobacter sp. TS-15
2.54
-
2-bromo-1-(3-chlorophenyl)-1-propanone
pH 7.5, 25C
Arthrobacter sp. TS-15
3.48
-
4-chlorobenzil
pH 7.5, 25C
Arthrobacter sp. TS-15
4.36
-
isatin
pH 7.5, 25C
Arthrobacter sp. TS-15
7.31
-
2-chloro-1-phenyl-1-propanone
pH 7.5, 25C
Arthrobacter sp. TS-15
7.31
-
2-chloro-1-phenyl-1-propane
pH 7.5, 25C
Arthrobacter sp. TS-15
7.94
-
1,2-naphthoquinone
pH 7.5, 25C
Arthrobacter sp. TS-15
9.49
-
Phenylglyoxal
pH 7.5, 25C
Arthrobacter sp. TS-15
15.37
-
1-Phenyl-1,3-butanedione
pH 7.5, 25C
Arthrobacter sp. TS-15
15.74
-
NADH
pH 7.5, 25C
Arthrobacter sp. TS-15
27.12
-
2-aminoacetophenone
pH 7.5, 25C
Arthrobacter sp. TS-15
27.13
-
2-aminoacetophenone
pH 7.5, 25C
Arthrobacter sp. TS-15
30.43
-
1-phenyl-1,2-propanedione
pH 7.5, 25C
Arthrobacter sp. TS-15
31.11
-
1,2-indanedione
pH 7.5, 25C
Arthrobacter sp. TS-15
31.11
-
1,2-indandione
pH 7.5, 25C
Arthrobacter sp. TS-15
37.38
-
ethyl 2-chlorobenzoylacetate
pH 7.5, 25C
Arthrobacter sp. TS-15
40.05
-
methyl benzoylformate
pH 7.5, 25C
Arthrobacter sp. TS-15
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Arthrobacter sp. TS-15
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7.5
8.5
highest stability
Arthrobacter sp. TS-15
7.5
8.5
t1/2: 34-21 h
Arthrobacter sp. TS-15
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
no apparent activity with NADP(H)+ and full catalytic activity with NAD(H). The preference for NADH makes the utilization of the enzyme an attractive for industrial applications, since NADH is more stable and less expensive than NADPH under operational reaction conditions
Arthrobacter sp. TS-15
NADH
no apparent activity with NADP(H)+ and full catalytic activity with NAD(H). The preference for NADH makes the utilization of the enzyme an attractive for industrial applications, since NADH is more stable and less expensive than NADPH under operational reaction conditions
Arthrobacter sp. TS-15
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.1
-
2-chloro-1-phenyl-1-propanone
pH 7.5, 25C
Arthrobacter sp. TS-15
0.1
-
2-chloro-1-phenyl-1-propane
pH 7.5, 25C
Arthrobacter sp. TS-15
0.16
-
ethyl 2-chlorobenzoylacetate
pH 7.5, 25C
Arthrobacter sp. TS-15
0.26
-
1-Phenyl-1,3-butanedione
pH 7.5, 25C
Arthrobacter sp. TS-15
1.46
-
methyl benzoylformate
pH 7.5, 25C
Arthrobacter sp. TS-15
1.53
-
2-aminoacetophenone
pH 7.5, 25C
Arthrobacter sp. TS-15
Application (protein specific)
Application
Commentary
Organism
industry
the wide substrate spectrum of these dehydrogenases, combined with their regio- and enantioselectivity, suggests a high potential for the industrial production of valuable chiral compounds
Arthrobacter sp. TS-15
synthesis
the wide substrate spectrum of these dehydrogenases, combined with their regio- and enantioselectivity, suggests a high potential for the industrial production of valuable chiral compounds
Arthrobacter sp. TS-15
synthesis
the enzyme catalyzes the oxidation of an isomer of ephedrine and the regio- and enantioselective reduction of sterically demanding substrate 1-phenyl-1,2-propanedione to give (R)-phenylacetylcarbinol
Arthrobacter sp. TS-15
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli T7 shuffle (DE3)
Arthrobacter sp. TS-15
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
no apparent activity with NADP(H)+ and full catalytic activity with NAD(H). The preference for NADH makes the utilization of the enzyme an attractive for industrial applications, since NADH is more stable and less expensive than NADPH under operational reaction conditions
Arthrobacter sp. TS-15
NADH
no apparent activity with NADP(H)+ and full catalytic activity with NAD(H). The preference for NADH makes the utilization of the enzyme an attractive for industrial applications, since NADH is more stable and less expensive than NADPH under operational reaction conditions
Arthrobacter sp. TS-15
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1-Phenyl-1,3-butanedione
-
Arthrobacter sp. TS-15
2-aminoacetophenone
-
Arthrobacter sp. TS-15
2-chloro-1-phenyl-1-propane
-
Arthrobacter sp. TS-15
2-chloro-1-phenyl-1-propanone
-
Arthrobacter sp. TS-15
ethyl 2-chlorobenzoylacetate
-
Arthrobacter sp. TS-15
methyl benzoylformate
-
Arthrobacter sp. TS-15
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.1
-
2-chloro-1-phenyl-1-propanone
pH 7.5, 25C
Arthrobacter sp. TS-15
0.1
-
2-chloro-1-phenyl-1-propane
pH 7.5, 25C
Arthrobacter sp. TS-15
0.16
-
ethyl 2-chlorobenzoylacetate
pH 7.5, 25C
Arthrobacter sp. TS-15
0.26
-
1-Phenyl-1,3-butanedione
pH 7.5, 25C
Arthrobacter sp. TS-15
1.46
-
methyl benzoylformate
pH 7.5, 25C
Arthrobacter sp. TS-15
1.53
-
2-aminoacetophenone
pH 7.5, 25C
Arthrobacter sp. TS-15
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
1-phenyl-1-propanone
pH 7.5, 25C
Arthrobacter sp. TS-15
0.02
-
2-chloro-1-phenyl-1-propanone
pH 7.5, 25C
Arthrobacter sp. TS-15
0.02
-
2-chloro-1-phenyl-1-propane
pH 7.5, 25C
Arthrobacter sp. TS-15
0.06
-
1-phenyl-1,2-propanedione
pH 7.5, 25C
Arthrobacter sp. TS-15
0.06
-
ethyl 2-chlorobenzoylacetate
pH 7.5, 25C
Arthrobacter sp. TS-15
0.07
-
NADH
pH 7.5, 25C
Arthrobacter sp. TS-15
0.1
-
1,2-naphthoquinone
pH 7.5, 25C
Arthrobacter sp. TS-15
0.17
-
4-chlorobenzil
pH 7.5, 25C
Arthrobacter sp. TS-15
0.19
-
methyl benzoylformate
pH 7.5, 25C
Arthrobacter sp. TS-15
0.2
-
1-Phenyl-1,3-butanedione
pH 7.5, 25C
Arthrobacter sp. TS-15
0.51
-
isatin
pH 7.5, 25C
Arthrobacter sp. TS-15
0.6
-
2-aminoacetophenone
pH 7.5, 25C
Arthrobacter sp. TS-15
2.29
-
1,2-indanedione
pH 7.5, 25C
Arthrobacter sp. TS-15
2.29
-
1,2-indandione
pH 7.5, 25C
Arthrobacter sp. TS-15
5.67
-
Phenylglyoxal
pH 7.5, 25C
Arthrobacter sp. TS-15
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25930
-
calculated from sequence
Arthrobacter sp. TS-15
27000
-
SDS-PAGE
Arthrobacter sp. TS-15
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(-)-(1R,2S)-ephedrine + NAD+
Arthrobacter sp. TS-15
-
(S)-2-methylamino-1-phenylpropan-1-one + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Arthrobacter sp. TS-15
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(-)-(1R,2S)-ephedrine + NAD+
-
752351
Arthrobacter sp. TS-15
(S)-2-methylamino-1-phenylpropan-1-one + NADH + H+
-
-
-
?
(-)-(1R,2S)-ephedrine + NAD+
the enzyme acts on a broad range of different aryl-alkyl ketones, such as haloketones, ketoamines, diketones, and ketoesters. It accepts various types of aryl groups including phenyl-, pyridyl-, thienyl-, and furyl-rings, but the presence of an aromatic ring is essential for the activity. In addition, the presence of a functional group on the alkyl chain, such as an amine, a halogen, or a ketone, is also crucial. The enzyme exhibits a strict Prelog enantioselectivity. When acting on diketones, it catalyses the reduction of only the keto group closest to the ring, with no further reduction to the diol. (-)-(1R,2S)-ephedrine i.e. (1R,2S)-1-phenyl-1-hydroxy-2-methylaminopropane. (S)-2-methylamino-1-phenylpropan-1-one i.e. (S)-methcathinone
752351
Arthrobacter sp. TS-15
(S)-2-methylamino-1-phenylpropan-1-one + NADH + H+
-
-
-
?
(4-chlorophenyl)-2-pyridinylmethanone + NAD+
specifc activity: 1.74 U/mg. Product: 70% (S) enantiomeric excess
752351
Arthrobacter sp. TS-15
(S)-(4-chlorophenyl)(pyridin-2-yl)methanol + NADH + H+
-
-
-
?
(4-chlorophenyl)-2-pyridinylmethanone + NADH + H+
2.7% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 70% enantiomeric excess
752351
Arthrobacter sp. TS-15
(R)-(4-chlorophenyl)(pyridin-2-yl)methanol + NAD+
-
-
-
?
1,2-indandione + NAD+
specifc 50.05 U/mg
752351
Arthrobacter sp. TS-15
? + NADH + H+
-
-
-
?
1,2-indanedione + NADH + H+
78.1% of the activity as compared to 1-phenyl-1,2-propanedione
752351
Arthrobacter sp. TS-15
1-hydroxy-1,3-dihydro-2H-inden-2-one + NAD+
-
-
-
?
1,2-naphthoquinone + NAD+
specifc activity: 13.89 U/mg
752351
Arthrobacter sp. TS-15
? + NADH + H+
-
-
-
?
1,2-naphthoquinone + NADH + H+
21.7% of the activity as compared to 1-phenyl-1,2-propanedione
752351
Arthrobacter sp. TS-15
1-hydroxynaphthalen-2(1H)-one + NAD+
-
-
-
?
1-phenyl-1,2-propanedione + NAD+
specifc activity: 64.02 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(R)-phenylacetylcarbinol + NADH + H+
-
-
-
?
1-phenyl-1,2-propanedione + NADH + H+
substrate with highest activity. Full conversion and enantiomeric excess of more than 99%
752351
Arthrobacter sp. TS-15
(R)-phenylacetylcarbinol + NAD+
-
-
-
?
1-phenyl-1,3-butanedione + NAD+
specifc activity: 13.64 U/mg. Product: 98% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(4R)-4-hydroxy-4-phenylbutan-2-one + NADH + H+
-
-
-
?
1-phenyl-1,3-butanedione + NADH + H+
21.3% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 98% enantiomeric excess
752351
Arthrobacter sp. TS-15
(4R)-4-hydroxy-4-phenylbutan-2-one + NAD+
-
-
-
?
1-phenyl-1-propanone + NAD+
specifc activity: 1.55 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-1-phenylpropan-1-ol + NADH + H+
-
-
-
?
1-phenyl-1-propanone + NADH + H+
2.4% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-1-phenylpropan-1-ol + NAD+
-
-
-
?
2,2'-dichlorobenzil + NAD+
specifc activity: 27.91 U/mg. Product: 94% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-1,2-bis(2-chlorophenyl)-2-hydroxyethan-1-one + NADH + H+
-
-
-
?
2,2'-dichlorobenzil + NADH + H+
43.6% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 4% enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-1,2-bis(2-chlorophenyl)-2-hydroxyethan-1-one + NAD+
-
-
-
?
2,2'-furil + NAD+
specifc activity: 19.95 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-1,2-di(furan-2-yl)-2-hydroxyethan + NADH + H+
-
-
-
?
2,2'-furil + NADH + H+
20% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-1,2-di(furan-2-yl)-2-hydroxyethan-1-one + NAD+
-
-
-
?
2,2'-thenil + NAD+
specifc activity: 46.77 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-2-hydroxy-1,2-di(thiophen-2-yl)ethan-1-one + NADH + H+
-
-
-
?
2,2'-thenil + NADH + H+
46.8% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-2-hydroxy-1,2-di(thiophen-2-yl)ethan-1-one + NAD+
-
-
-
?
2-aminoacetophenone + NAD+
specifc activity: 25.79 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-2-amino-1-phenylethan-1-ol + NADH + H+
-
-
-
?
2-aminoacetophenone + NADH + H+
40.3% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-2-amino-1-phenylethan-1-ol + NAD+
-
-
-
?
2-bromo-1-(3-chlorophenyl)-1-propanone + NAD+
specifc activity: 5.1 U/mg
752351
Arthrobacter sp. TS-15
(R,S)-2-bromo-1-(3-chlorophenyl)propan-1-ol + NADH + H+
-
-
-
?
2-bromo-1-(3-chlorophenyl)-1-propanone + NADH + H+
7.9% of the activity as compared to 1-phenyl-1,2-propanedione
752351
Arthrobacter sp. TS-15
2-bromo-1-(3-chlorophenyl)propan-1-ol + NAD+
-
-
-
?
2-bromoacetophenone + NAD+
specifc activity: 9.44 U/mg. Product: 85% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-2-bromo-1-phenylethan-1-ol + NADH + H+
-
-
-
?
2-bromoacetophenone + NADH + H+
14.8% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 85% enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-2-bromo-1-phenylethan-1-ol + NAD+
-
-
-
?
2-chloro-1-phenyl-1-propane + NAD+
specifc activity: 8.0 U/mg
752351
Arthrobacter sp. TS-15
(R,S)-2-chloro-1-phenylpropan-1-ol + NADH + H+
-
-
-
?
2-chloro-1-phenyl-1-propanone + NADH + H+
12.5% of the activity as compared to 1-phenyl-1,2-propanedione
752351
Arthrobacter sp. TS-15
2-chloro-1-phenyl-1-propan-1-ol + NAD+
-
-
-
?
2-chloroacetophenone + NAD+
specifc activity: 8.71 U/mg. Product: 98% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-2-chloro-1-phenylethan-1-ol + NADH + H+
-
-
-
?
2-chloroacetophenone + NADH + H+
13.6% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 98% enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-2-chloro-1-phenylethan-1-ol + NAD+
-
-
-
?
2-thenoylacetonitrile + NAD+
specifc activity: 6.87 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(3R)-3-hydroxy-3-(thiophen-2-yl)propanenitril + NADH + H+
-
-
-
?
2-thenoylacetonitrile + NADH + H+
6.87% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-hydroxy(thiophen-2-yl)acetonitrile + NAD+
-
-
-
?
3-chloropropiophenone + NAD+
specifc activity: 4.45 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-3-chloro-1-phenylpropan-1-ol + NADH + H+
-
-
-
?
3-chloropropiophenone + NADH + H+
6.9% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(1R)-3-chloro-1-phenylpropan-1-ol + NAD+
-
-
-
?
4,4'-difluorobenzil + NAD+
specifc activity: 48.2 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-1,2-bis(4-fluorophenyl)-2-hydroxyethan-1-one + NADH + H+
-
-
-
?
4,4'-difluorobenzil + NADH + H+
75.3% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-1,2-bis(4-fluorophenyl)-2-hydroxyethan-1-one + NAD+
-
-
-
?
4,4'-dimethylbenzil + NAD+
specifc activity: 3.12 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-2-hydroxy-1,2-bis(4-methylphenyl)ethan-1-one + NADH + H+
-
-
-
?
4,4'-dimethylbenzil + NADH + H+
4.8% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-1,2-bis(4-methylphenyl)-2-hydroxyethan-1-one + NAD+
-
-
-
?
4-chlorobenzil + NAD+
specifc activity: 6.5 U/mg. Product: 95% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
? + NADH + H+
-
-
-
?
4-chlorobenzil + NADH + H+
10.2% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 58% enantiomeric excess
752351
Arthrobacter sp. TS-15
? + NAD+
-
-
-
?
benzil + NAD+
specifc activity: 47.06 U/mg. Product: 99% enantiomeric (R) excess
752351
Arthrobacter sp. TS-15
(2R)-2-hydroxy-1,2-diphenylethan-1-one + NADH + H+
-
-
-
?
benzil + NADH + H+
73.5% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-2-hydroxy-1,2-diphenylethan-1-one + NADH + H+
?
-
-
?
ethyl 2-chlorobenzoylacetate + NAD+
specifc activity: 35.21 U/mg
752351
Arthrobacter sp. TS-15
ethyl (R,S)-2-chloro-3-hydroxy-3-phenylpropanoate + NADH + H+
-
-
-
?
ethyl 2-chlorobenzoylacetate + NADH + H+
54.9% of the activity as compared to 1-phenyl-1,2-propanedione
752351
Arthrobacter sp. TS-15
ethyl 2-chloro-3-hydroxy-3-phenylpropanoate + NAD+
-
-
-
?
isatin + NAD+
specifc activity: 7.84 U/mg. Product: 52% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
? + NADH + H+
-
-
-
?
isatin + NADH + H+
12.25% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 52% enantiomeric excess
752351
Arthrobacter sp. TS-15
(3R)-3-hydroxy-1,3-dihydro-2H-indol-2-one + NAD+
-
-
-
?
methyl benzoylformate + NAD+
specifc activity: 46.56 U/mg. Product: 98% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
methyl (2R)-hydroxy(phenyl)acetate + NADH + H+
-
-
-
?
methyl benzoylformate + NADH + H+
72.7% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 98% enantiomeric excess
752351
Arthrobacter sp. TS-15
methyl (2S)-hydroxy(phenyl)acetate + NAD+
-
-
-
?
phenyl-2-pyridinylmethanone + NAD+
specifc activity: 2.81 U/mg. Product: 24% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(R)-phenyl(pyridin-2-yl)methanol + NADH + H+
-
-
-
?
phenyl-2-pyridinylmethanone + NADH + H+
4.4% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 24% enantiomeric excess
752351
Arthrobacter sp. TS-15
(R)-phenyl(pyridin-2-yl)methanol + NAD+
-
-
-
?
phenylglyoxal + NAD+
specifc activity: 12.1 U/mg. Product: 99% (R) enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-hydroxy(phenyl)acetaldehyde + NADH + H+
-
-
-
?
phenylglyoxal + NADH + H+
18.9% of the activity as compared to 1-phenyl-1,2-propanedione. The (R)-enantiomer is formed with 99% enantiomeric excess
752351
Arthrobacter sp. TS-15
(2R)-hydroxy(phenyl)acetaldehyde + NAD+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
4 * 27000, SDS-PAGE, the smallest oligomeric state seems to be a dimer, the predominant oligomeric state is the tetramer
Arthrobacter sp. TS-15
tetramer
4 * 27000, SDS-PAGE, the smallest oligomeric state seems to be a dimer, the predominant oligomeric state is the tetramer
Arthrobacter sp. TS-15
tetramer
4 * 27000, the enzyme tends to form higher oligomeric states, up to a hexadecamer, SDS-PAGE
Arthrobacter sp. TS-15
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
70
-
-
Arthrobacter sp. TS-15
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
55
75
55C: about 40% of maximal activity, 75C: about 75% of maximal activity
Arthrobacter sp. TS-15
65
75
65C: about 55% of maximal activity, 75C: about 75% of maximal activity
Arthrobacter sp. TS-15
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
5
-
half-life: 2.6 h
Arthrobacter sp. TS-15
25
-
half-life: 38.3 h
Arthrobacter sp. TS-15
40
-
half-life: 82.8 h
Arthrobacter sp. TS-15
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.77
-
1-phenyl-1-propanone
pH 7.5, 25C
Arthrobacter sp. TS-15
2.54
-
2-bromo-1-(3-chlorophenyl)-1-propanone
pH 7.5, 25C
Arthrobacter sp. TS-15
3.48
-
4-chlorobenzil
pH 7.5, 25C
Arthrobacter sp. TS-15
4.36
-
isatin
pH 7.5, 25C
Arthrobacter sp. TS-15
7.31
-
2-chloro-1-phenyl-1-propanone
pH 7.5, 25C
Arthrobacter sp. TS-15
7.31
-
2-chloro-1-phenyl-1-propane
pH 7.5, 25C
Arthrobacter sp. TS-15
7.94
-
1,2-naphthoquinone
pH 7.5, 25C
Arthrobacter sp. TS-15
9.49
-
Phenylglyoxal
pH 7.5, 25C
Arthrobacter sp. TS-15
15.37
-
1-Phenyl-1,3-butanedione
pH 7.5, 25C
Arthrobacter sp. TS-15
15.74
-
NADH
pH 7.5, 25C
Arthrobacter sp. TS-15
27.12
-
2-aminoacetophenone
pH 7.5, 25C
Arthrobacter sp. TS-15
27.13
-
2-aminoacetophenone
pH 7.5, 25C
Arthrobacter sp. TS-15
30.43
-
1-phenyl-1,2-propanedione
pH 7.5, 25C
Arthrobacter sp. TS-15
31.11
-
1,2-indanedione
pH 7.5, 25C
Arthrobacter sp. TS-15
31.11
-
1,2-indandione
pH 7.5, 25C
Arthrobacter sp. TS-15
37.38
-
ethyl 2-chlorobenzoylacetate
pH 7.5, 25C
Arthrobacter sp. TS-15
40.05
-
methyl benzoylformate
pH 7.5, 25C
Arthrobacter sp. TS-15
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Arthrobacter sp. TS-15
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7.5
8.5
highest stability
Arthrobacter sp. TS-15
7.5
8.5
t1/2: 34-21 h
Arthrobacter sp. TS-15
Other publictions for EC 1.1.1.423
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
755862
Shanati
Biodegradation of ephedrine i ...
Arthrobacter sp. TS-15
Appl. Environ. Microbiol.
86
e02487-19
2020
-
-
-
-
-
-
2
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
-
-
2
2
1
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
752351
Shanati
-
Two enantiocomplementary ephe ...
Arthrobacter sp. TS-15
ACS Catal.
9
6202-6211
2019
-
3
1
-
-
-
6
15
-
-
2
1
-
1
-
-
1
-
-
-
-
-
52
3
1
1
2
3
17
1
-
2
2
6
-
-
-
3
1
2
-
-
-
-
6
6
15
-
-
2
1
-
-
-
1
-
-
-
-
52
3
1
2
3
17
1
-
2
-
-
-
-
-
-
-