Any feedback?
Literature summary for 1.1.1.420 extracted from
- D-apiose reductase from Aerobacter aerogenes (1970), J. Bacteriol., 101, 910-915 .
General Stability
| General Stability | Organism |
|---|---|
| the presence of 0.06 M mercaptoethanol increases the stability of the enzyme when stored at 0-4 °C | Klebsiella aerogenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzymatic reduction of D-apiose is not appreciably affected by the presence of 0.02 M EDTA | Klebsiella aerogenes |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
D-apiitol | at pH 9.5 and 25°C | Klebsiella aerogenes |  |
| 0.02 | - |
D-apiose | at pH 7.5 and 25°C | Klebsiella aerogenes | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-apiose + NADH + H+ | Klebsiella aerogenes | - |
D-apiitol + NAD+ | - |
r | |
| additional information | Klebsiella aerogenes | the enzyme is almost completely specific for the reduced and oxidized forms of nicotinamide adenine dinucleotide. The activity obtained with NADPH and NADP+ is 1.5 and 0.9%, respectively, of that obtained with NADH and NAD+ | ? | - |
- |
|
| additional information | Klebsiella aerogenes PRL-R3 | the enzyme is almost completely specific for the reduced and oxidized forms of nicotinamide adenine dinucleotide. The activity obtained with NADPH and NADP+ is 1.5 and 0.9%, respectively, of that obtained with NADH and NAD+ | ? | - |
- |
|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
- |
- |
| Klebsiella aerogenes PRL-R3 | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, storage medium, 24 h, 10-20% loss of activity | Klebsiella aerogenes |
| 0-4°C, storage medium, 48 h, 50% loss of activity | Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-apiitol + NAD+ | - |
Klebsiella aerogenes | D-apiose + NADH + H+ | - |
r | |
| D-apiose + NADH + H+ | - |
Klebsiella aerogenes | D-apiitol + NAD+ | - |
r | |
| D-erythrose + NADH + H+ | 1.5% activity compared to D-apiose | Klebsiella aerogenes | ? | - |
? | |
| D-mannitol + NAD+ | 1.0% activity compared to D-apiitol | Klebsiella aerogenes | ? | - |
? | |
| D-mannitol + NAD+ | 1.0% activity compared to D-apiitol | Klebsiella aerogenes PRL-R3 | ? | - |
? | |
| D-ribulose + NADH + H+ | 8.5% activity compared to D-apiose | Klebsiella aerogenes | ? | - |
? | |
| D-ribulose + NADH + H+ | 8.5% activity compared to D-apiose | Klebsiella aerogenes PRL-R3 | ? | - |
? | |
| erythritol + NAD+ | 1.0% activity compared to D-apiitol | Klebsiella aerogenes | ? | - |
? | |
| erythritol + NAD+ | 1.0% activity compared to D-apiitol | Klebsiella aerogenes PRL-R3 | ? | - |
? | |
| additional information | the enzyme is almost completely specific for the reduced and oxidized forms of nicotinamide adenine dinucleotide. The activity obtained with NADPH and NADP+ is 1.5 and 0.9%, respectively, of that obtained with NADH and NAD+ | Klebsiella aerogenes | ? | - |
- |
|
| additional information | no activity is obtained with D-glucose, D-mannose, D-galactose, D-fructose, L-rhamnose, L-fucose, D-ribose, D-xylose, L-arabinose, D-arabinose, D-xylulose, D-glyceraldehyde, galactitol, sorbitol, xylitol, L-arabitol, D-arabitol, and glycerol | Klebsiella aerogenes | ? | - |
- |
|
| additional information | the enzyme is almost completely specific for the reduced and oxidized forms of nicotinamide adenine dinucleotide. The activity obtained with NADPH and NADP+ is 1.5 and 0.9%, respectively, of that obtained with NADH and NAD+ | Klebsiella aerogenes PRL-R3 | ? | - |
- |
|
| additional information | no activity is obtained with D-glucose, D-mannose, D-galactose, D-fructose, L-rhamnose, L-fucose, D-ribose, D-xylose, L-arabinose, D-arabinose, D-xylulose, D-glyceraldehyde, galactitol, sorbitol, xylitol, L-arabitol, D-arabitol, and glycerol | Klebsiella aerogenes PRL-R3 | ? | - |
- |
|
| ribitol + NAD+ | 5.5% activity compared to D-apiitol | Klebsiella aerogenes | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-apiose reductase | - |
Klebsiella aerogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
optimal activity for the reduction of D-apiose is obtained at pH 7.5 in sodium phosphate buffer | Klebsiella aerogenes |
| 7.5 | - |
optimal activity for the reduction of D-apiose is obtained at pH 7.5 in glycylglycine buffer | Klebsiella aerogenes |
| 9.5 | - |
optimal activity for the oxidation of D-apiitol is obtained at pH 10.5 in glycylglycine buffer | Klebsiella aerogenes |
| 10.5 | - |
optimal activity for the oxidation of D-apiitol is obtained at pH 10.5 in glycine buffer | Klebsiella aerogenes |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Klebsiella aerogenes | |
| NADH | - |
Klebsiella aerogenes | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
