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Literature summary for 1.1.1.42 extracted from

  • Xu, Y.; Liu, L.; Nakamura, A.; Someya, S.; Miyakawa, T.; Tanokura, M.
    Studies on the regulatory mechanism of isocitrate dehydrogenase 2 using acetylation mimics (2017), Sci. Rep., 7, 9785 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.42

Activating Compound

Activating Compound Comment Organism Structure
SIRT3 under calorie restriction, mitochondrial deacetylase Sirt3 deacetylates and activates isoform IDH2 Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli KRX cells Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
wild type and mutant enzyme K256Q, sitting drop vapor diffusion method, using 0.1 M sodium citrate tribasic dehydrate (pH 5.6), 11.5% (v/v) 2-propanol and 10.8% (w/v) PEG 4000 Mus musculus

Protein Variants

Protein Variants Comment Organism
K106Q the activity of the mutant is about 118% of the wild type value Mus musculus
K155Q the activity of the mutant is about 110% of the wild type value Mus musculus
K166Q the activity of the mutant is about 80% of the wild type value Mus musculus
K180Q the activity of the mutant is about 60% of the wild type value Mus musculus
K243Q the activity of the mutant is about 90% of the wild type value Mus musculus
K251Q inactive Mus musculus
K256Q the activity of the mutant is about 40% of the wild type value Mus musculus
K263Q the activity of the mutant is about 80% of the wild type value Mus musculus
K272Q the activity of the mutant is about 65% of the wild type value Mus musculus
K275Q the activity of the mutant is about 85% of the wild type value Mus musculus
K282Q the activity of the mutant is about 105% of the wild type value Mus musculus
K360Q the mutant shows wild type activity Mus musculus
K384Q the activity of the mutant is about 90% of the wild type value Mus musculus
K413Q the activity of the mutant is about 20% of the wild type value Mus musculus
K442Q the activity of the mutant is about 90% of the wild type value Mus musculus
K80Q the activity of the mutant is about 105% of the wild type value Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0148
-
 NADP+ mutant K256Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus
0.0304
-
 isocitrate mutant K256Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus
0.0337
-
 NADP+ wild type isoform IDH2, at pH 7.4 and 25°C Mus musculus
0.0544
-
 isocitrate wild type isoform IDH2, at pH 7.4 and 25°C Mus musculus
0.0555
-
 isocitrate mutant K413Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus
0.1122
-
 NADP+ mutant K413Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Mus musculus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1 mM used in assay conditions Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Mus musculus
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus P54071
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography and Resource S column chromatography Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
 Mus musculus 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
IDH2
-
 Mus musculus
isocitrate dehydrogenase 2
-
 Mus musculus
NADP+-dependent IDH
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.66
-
 isocitrate mutant K256Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus
3.13
-
 NADP+ mutant K256Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus
4.61
-
 isocitrate mutant K413Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus
5.6
-
 isocitrate wild type isoform IDH2, at pH 7.4 and 25°C Mus musculus
6.36
-
 NADP+ wild type isoform IDH2, at pH 7.4 and 25°C Mus musculus
7.69
-
 NADP+ mutant K413Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
59
-
 NADP+ mutant K413Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus
83
-
 isocitrate mutant K413Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus
88
-
 isocitrate mutant K256Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus
111
-
 isocitrate wild type isoform IDH2, at pH 7.4 and 25°C Mus musculus
186
-
 NADP+ wild type isoform IDH2, at pH 7.4 and 25°C Mus musculus
205
-
 NADP+ mutant K256Q of isoform IDH2, at pH 7.4 and 25°C Mus musculus