Cloned (Comment) | Organism |
---|---|
expressipon in Escherichia coli | Acinetobacter baumannii |
Protein Variants | Comment | Organism |
---|---|---|
S113A | mutant enzyme with remarkably reduced enzymatic activity | Acinetobacter baumannii |
S113A | the mutation remarkably reduces the enzymatic activity | Acinetobacter baumannii |
S113D | the mutant lacks almost all enzymatic activity | Acinetobacter baumannii |
S113D | the phosphorylation-mimicking mutant lacks almost all enzymatic activity | Acinetobacter baumannii |
S113E | the mutant lacks almost all enzymatic activity | Acinetobacter baumannii |
S113E | the phosphorylation-mimicking mutant lacks almost all enzymatic activity | Acinetobacter baumannii |
S113G | mutant enzyme with remarkably reduced enzymatic activity | Acinetobacter baumannii |
S113G | the mutation remarkably reduces the enzymatic activity | Acinetobacter baumannii |
S113T | mutant enzyme with remarkably reduced enzymatic activity | Acinetobacter baumannii |
S113T | the mutation remarkably reduces the enzymatic activity | Acinetobacter baumannii |
S113Y | mutant enzyme with remarkably reduced enzymatic activity | Acinetobacter baumannii |
S113Y | the mutation remarkably reduces the enzymatic activity | Acinetobacter baumannii |
General Stability | Organism |
---|---|
the activity of the enzyme treated with Escherichia coli isocitrate dehydrogenase kinase/phosphatase immediately decreases by 80% within 9 min | Acinetobacter baumannii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0181 | - |
NADP+ | in the presence of Mg2+, pH and temperature not specified in the publication | Acinetobacter baumannii | |
0.0181 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme | Acinetobacter baumannii | |
0.0223 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H | Acinetobacter baumannii | |
0.0261 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A | Acinetobacter baumannii | |
0.0289 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E | Acinetobacter baumannii | |
0.0466 | - |
NADP+ | in the presence of Mn2+, pH and temperature not specified in the publication | Acinetobacter baumannii | |
0.0466 | - |
NADP+ | pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme | Acinetobacter baumannii | |
0.0505 | - |
isocitrate | in the presence of Mn2+, pH and temperature not specified in the publication | Acinetobacter baumannii | |
0.0505 | - |
isocitrate | pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme | Acinetobacter baumannii | |
0.0654 | - |
isocitrate | in the presence of Mg2+, pH and temperature not specified in the publication | Acinetobacter baumannii | |
0.0654 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme | Acinetobacter baumannii | |
0.0879 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T | Acinetobacter baumannii | |
0.1043 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A | Acinetobacter baumannii | |
0.1169 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H | Acinetobacter baumannii | |
0.1221 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A | Acinetobacter baumannii | |
0.1414 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E | Acinetobacter baumannii | |
0.2447 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T | Acinetobacter baumannii | |
0.3276 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A | Acinetobacter baumannii | |
0.8546 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G | Acinetobacter baumannii | |
2.525 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G | Acinetobacter baumannii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Acinetobacter baumannii | |
Mn2+ | activates | Acinetobacter baumannii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83500 | - |
gel filtration | Acinetobacter baumannii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Acinetobacter baumannii | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | Acinetobacter baumannii | overall reaction | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baumannii | - |
- |
- |
Acinetobacter baumannii | V5VCI0 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme is phosphorylated at Ser113 by Escherichia coli isocitrate dehydrogenase kinase/phosphatase | Acinetobacter baumannii |
Purification (Comment) | Organism |
---|---|
- |
Acinetobacter baumannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Acinetobacter baumannii | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | overall reaction | Acinetobacter baumannii | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
additional information | the enzyme is a substrate for Escherichia coli isocitrate dehydrogenase kinase/phosphatase | Acinetobacter baumannii | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 45000, SDS-PAGE | Acinetobacter baumannii |
homodimer | 2 * 83500, SDS-PAGE | Acinetobacter baumannii |
Synonyms | Comment | Organism |
---|---|---|
AbIDH1 | - |
Acinetobacter baumannii |
IDH1 | - |
Acinetobacter baumannii |
isocitrate dehydrogenase 1 | - |
Acinetobacter baumannii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A | Acinetobacter baumannii | |
2.1 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A | Acinetobacter baumannii | |
2.9 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T | Acinetobacter baumannii | |
3 | 6 | isocitrate | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E | Acinetobacter baumannii | |
3.2 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T | Acinetobacter baumannii | |
3.4 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G | Acinetobacter baumannii | |
4 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G | Acinetobacter baumannii | |
28.5 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A | Acinetobacter baumannii | |
29.2 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H | Acinetobacter baumannii | |
29.8 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme | Acinetobacter baumannii | |
30.3 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E | Acinetobacter baumannii | |
31.3 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A | Acinetobacter baumannii | |
33.4 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme | Acinetobacter baumannii | |
37 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H | Acinetobacter baumannii | |
50.4 | - |
isocitrate | pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme | Acinetobacter baumannii | |
60.1 | - |
NADP+ | pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme | Acinetobacter baumannii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | dependent on | Acinetobacter baumannii | |
NADP+ | NADP+-dependent enzyme | Acinetobacter baumannii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E | Acinetobacter baumannii | |
1.1 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A | Acinetobacter baumannii | |
1.3 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H | Acinetobacter baumannii | |
2 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G | Acinetobacter baumannii | |
4 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G | Acinetobacter baumannii | |
6 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A | Acinetobacter baumannii | |
13 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T | Acinetobacter baumannii | |
16 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A | Acinetobacter baumannii | |
33 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T | Acinetobacter baumannii | |
250 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E | Acinetobacter baumannii | |
300 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A | Acinetobacter baumannii | |
320 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H | Acinetobacter baumannii | |
500 | - |
isocitrate | pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme | Acinetobacter baumannii | |
1000 | - |
isocitrate | pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme | Acinetobacter baumannii | |
1300 | - |
NADP+ | pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme | Acinetobacter baumannii | |
1700 | - |
NADP+ | pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme | Acinetobacter baumannii |