Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
C363S | the mutation does not perturb the enzymatic characteristics of the enzyme. However, the mutant is less affected by nitrosoglutathione treatment than the wild type enzyme | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GSSG | increasing GSSG concentrations progressively inhibit the enzyme activity, almost complete inhibition at 1 mM | Arabidopsis thaliana | |
additional information | increasing concentrations of H2O2 hardly affect the enzyme activity | Arabidopsis thaliana | |
nitrosoglutathione | increasing nitrosoglutathione concentrations progressively inhibit the enzyme activity, complete inhibition at 2 mM | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
NADP+ | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.095 | - |
isocitrate | mutant enzyme C3636S, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.099 | - |
isocitrate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arabidopsis thaliana | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM used in assay conditions | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Arabidopsis thaliana | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SRZ6 | ecotype Columbia-0 | - |
Oxidation Stability | Organism |
---|---|
enzyme activity is affected by oxidative agents like oxidized glutathione and nitrosoglutathione through modifications of conserved Cys residues in the protein | Arabidopsis thaliana |
Posttranslational Modification | Comment | Organism |
---|---|---|
glutathionylation | S-glutathionylation of Cys363 is involved in the inhibition of the cytosolic enzyme activity upon nitrosoglutathione treatments | Arabidopsis thaliana |
S-nitrosylation | - |
Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
Ni2+-Sepharose column chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Arabidopsis thaliana | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 48300, His-tagged enzyme, calculated from amino acid sequence | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
cICDH | - |
Arabidopsis thaliana |
NADP-dependent isocitrate dehydrogenase | - |
Arabidopsis thaliana |
NADP-ICDH | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.31 | - |
isocitrate | mutant enzyme C3636S, at pH 7.5 and 25°C | Arabidopsis thaliana | |
4.93 | - |
isocitrate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
5.81 | - |
NADP+ | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | high activity between pH 7.5 and 8.5 | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
45.4 | - |
isocitrate | mutant enzyme C3636S, at pH 7.5 and 25°C | Arabidopsis thaliana | |
49.7 | - |
isocitrate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana |