Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion technique with reservoir solution consisting of 0.6 M ZnSO4 and 0.1 M Na cacodylate, pH 6.3 | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-values of wild-type and chimeric enzymes | Escherichia coli | |
additional information | - |
additional information | Km-values of wild-type and chimeric enzymes | Archaeoglobus fulgidus | |
0.0165 | - |
NADP+ | 60°C, pH 8.0 | Archaeoglobus fulgidus | |
0.0392 | - |
NADP+ | 40°C, pH 8.0 | Escherichia coli | |
0.0405 | - |
isocitrate | 40°C, pH 8.0 | Escherichia coli | |
0.332 | - |
isocitrate | 60°C, pH 8.0 | Archaeoglobus fulgidus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | a zinc ion is tightly bound to Asp301, Asp305, Asp277 in the active site of subunit A and subunit B | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45800 | - |
- |
Escherichia coli |
45800 | - |
- |
Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O29610 | - |
- |
Escherichia coli | P08200 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Escherichia coli | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | - |
Archaeoglobus fulgidus | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AfIDH | - |
Archaeoglobus fulgidus |
EcIDH | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Escherichia coli |
50 | - |
- |
Escherichia coli |
60 | - |
assay at | Archaeoglobus fulgidus |
90 | - |
about | Archaeoglobus fulgidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal stability of wild-type and chimeric enzymes | Escherichia coli |
additional information | - |
thermal stability of wild-type and chimeric enzymes | Archaeoglobus fulgidus |
50 | - |
t1/2: 24.2 min | Escherichia coli |
65 | - |
t1/2: 167 min | Archaeoglobus fulgidus |
70 | - |
t1/2: 106 min | Archaeoglobus fulgidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover numbers of wild-type and chimeric enzymes | Escherichia coli | |
additional information | - |
additional information | turnover numbers of wild-type and chimeric enzymes | Archaeoglobus fulgidus | |
88.1 | - |
NADP+ | 40°C, pH 8.0 | Escherichia coli | |
106.4 | - |
isocitrate | 40°C, pH 8.0 | Escherichia coli | |
219 | - |
NADP+ | 60°C, pH 8.0 | Archaeoglobus fulgidus | |
255 | - |
isocitrate | 60°C, pH 8.0 | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
8 | - |
assay at | Archaeoglobus fulgidus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover numbers of wild-type and chimeric enzymes | Escherichia coli | |
additional information | - |
additional information | turnover numbers of wild-type and chimeric enzymes | Archaeoglobus fulgidus | |
700 | - |
isocitrate | 60°C, pH 8.0 | Archaeoglobus fulgidus | |
2200 | - |
NADP+ | 40°C, pH 8.0 | Escherichia coli | |
2600 | - |
isocitrate | 40°C, pH 8.0 | Escherichia coli | |
13300 | - |
NADP+ | 60°C, pH 8.0 | Archaeoglobus fulgidus |